A5020680319- SARS-CoV-2 and COVID-19 Research
- Respiratory viral infections research
- Viral gastroenteritis research and epidemiology
- COVID-19 Clinical Research Studies
- Pneumonia and Respiratory Infections
- Vaccine Coverage and Hesitancy
- Mosquito-borne diseases and control
- Bacterial Infections and Vaccines
- Viral Infections and Vectors
- Influenza Virus Research Studies
- Malaria Research and Control
- Immunotherapy and Immune Responses
- Virus-based gene therapy research
- Bacillus and Francisella bacterial research
- Pneumocystis jirovecii pneumonia detection and treatment
- Viral Infectious Diseases and Gene Expression in Insects
- Virology and Viral Diseases
- vaccines and immunoinformatics approaches
- Bacteriophages and microbial interactions
- Analytical Chemistry and Chromatography
- Cell Image Analysis Techniques
- COVID-19 and Mental Health
- Heparin-Induced Thrombocytopenia and Thrombosis
- SARS-CoV-2 detection and testing
- Immune Cell Function and Interaction
Sanofi (United States)
2012-2021
Clover Biopharmaceuticals (China)
2020-2021
Pfizer (United States)
2011
Centers for Disease Control and Prevention
2010
National Center for Immunization and Respiratory Diseases
2010
University of Alabama at Birmingham
2010
University College London
2010
Finnish Institute for Health and Welfare
2010
Pearl River Community College
2005-2007
Human Immunome Project
1998-2004
As part of the accelerated development vaccines against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), we report a dose-finding and adjuvant justification study SCB-2019, protein subunit vaccine candidate containing stabilised trimeric form spike (S)-protein (S-Trimer) combined with two different adjuvants.Our is phase 1, randomised, double-blind placebo-controlled trial at specialised clinical trials centre in Australia. We enrolled healthy adult volunteers age groups:...
A dengue plaque reduction neutralization test (PRNT) to measure serotype-specific neutralizing antibodies for all four virus serotypes was developed, optimized, and validated in accordance with guidelines validation of bioanalytical methods using human serum samples from dengue-infected persons receiving a vaccine candidate. Production characterization challenge viruses used the assay standardized. Once stocks were characterized, PRNT(50) each optimized according factorial design experiments...
Although a number of studies have investigated and quantified immune correlates protection against influenza in adults children, data on the elderly are sparse. A recent vaccine efficacy trial comparing standard-dose with high-dose inactivated persons 65 years age older provided opportunity to examine relationship between values three immunologic assays community-acquired A/H3N2 illness. The induced significantly higher antibody titers than for all assays. For hemagglutination inhibition...
Rotavirus subunit vaccines are being evaluated for use in humans. The virus-like particles (VLPs) these produced insect cells coinfected with combinations of baculovirus recombinants expressing bovine RF VP2 and simian SAll VP4, VP6, or VP7 rotavirus proteins. VLPs were administered parenterally to mice rabbits, the immunogenicity protective efficacy evaluated. Rabbits vaccinated VP2/4/617 VP2/617 VLP developed high levels rotavirus-specific serum antibody fecal IgG but not IgA. induction...
We evaluated immunogenicity of SCB-2019, a subunit vaccine candidate containing pre-fusion trimeric form the SARS-CoV-2 spike (S)-protein adjuvanted with CpG-1018/alum.The phase 2/3, double-blind, randomized SPECTRA trial was conducted in five countries participants aged ≥ 18 years, either SARS-CoV-2-naïve or previously exposed. Participants were randomly assigned to receive two doses SCB-2019 placebo administered intramuscularly 21 days apart. In 2 part study, on 1, 22, and 36, neutralizing...
ABSTRACT Streptococcus pneumoniae (pneumococcus) polysaccharide serotype-specific antibodies that have opsonophagocytic activity are considered a primary mechanism of host defense against pneumococcal disease. In vitro assays (OPAs) with antibody and complement to mediate killing bacteria been designed developed as an adjunct the standardized serum immunoglobulin G antipneumococcal capsular enzyme immunoassay assess effectiveness vaccines. OPA presents challenges for assay standardization...
Antibody-mediated killing of Streptococcus pneumoniae (pneumococcus) by phagocytes is an important mechanism protection the human host against pneumococcal infections. Measurement opsonophagocytic antibodies use a standardized assay (OPA) for evaluation candidate vaccines and required licensure new conjugate vaccine formulations. We assessed agreement among six laboratories that used their own optimized OPAs on panel 16 reference sera 13 serotypes. Consensus titers, estimated using...
ABSTRACT Weight-based immunoglobulin G (IgG), IgM, IgA, and total Ig antibody assignments were made to human antipneumococcal standard reference serum lot 89-S, also known as 89-SF, for Streptococcus pneumoniae capsular polysaccharide (PnPs) serotypes 2, 6A, 8, 9N, 10A, 11A, 12F, 15B, 19A, 17F, 20, 22F, 33F, well C-polysaccharide (C-Ps), extending the standard's usefulness pneumococcal vaccine evaluation beyond original serotype 1, 3, 4, 5, 6B, 7F, 9V, 14, 18C, 19F, 23F (S. A. Quataert, C....
We assessed the non-inferiority of homologous boosting compared with heterologous recombinant protein vaccine, SCB-2019, in adults previously immunized different COVID-19 vaccines. Three equal cohorts (N ~ 420) Philippino (18-80 years) Comirnaty, CoronaVac or Vaxzevria vaccines were randomized 1:1 to receive (SCB-2019) boosters. Neutralizing antibodies against prototype SARS-CoV-2 (Wuhan-Hu-1) measured all participants and Delta variant Omicron sub-lineages subsets (30‒50 per arm) 15 days...
Two large pivotal phase III studies demonstrated the efficacy of tetravalent dengue vaccine (CYD-TDV; Dengvaxia®, Sanofi Pasteur) against all serotypes. Here we present an unprecedented integrated summary immunogenicity CYD-TDV to identify parameters driving neutralizing humoral immune response and evolution over time. We summarized profiles a 3-dose schedule administered 6 months apart across 10 II trials undertaken in endemic non-endemic countries. Dengue antibody titers sera were...
The rabies rapid fluorescent focus inhibition test (RFFIT) is the most widely used cell-based assay for detecting and quantitating virus neutralizing antibodies (RVNA) in human serum. However, it a complex, labor intensive, somewhat subjective manual assay, performance of which may be affected by number factors including quality cells virus, variability reagents skill expertise analysts. This study sought to identify evaluate conditions that impact RFFIT RVNA detection evaluating parameters...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTHeterogeneity of soybean seed proteins: one-dimensional electrophoretic profiles six different solubility fractionsBranda Hu and Asim EsenCite this: J. Agric. Food Chem. 1981, 29, 3, 497–501Publication Date (Print):May 1, 1981Publication History Published online1 May 2002Published inissue 1 1981https://pubs.acs.org/doi/10.1021/jf00105a015https://doi.org/10.1021/jf00105a015research-articleACS PublicationsRequest reuse permissionsArticle...
During clinical development of the licensed Japanese encephalitis chimeric virus vaccine (JE-CV), neutralization capacity vaccine-induced antibodies was assessed against and well characterized wild-type (wt) viruses isolated between 1949–1991. We whether JE-CV-induced can also neutralize more recent wt (JEV) isolates including a genotype 1 isolate. Sera from 12–18 month-old children who received single dose JE-CV in phase III study Thailand Philippines (ClinicalTrials.gov NCT00735644) were...
When the three major structural proteins, VP2, VP6, and VP7, of rotavirus are co-expressed in insect cells infected with recombinant baculoviruses, they self-assemble into triple-layered virus-like particles (VLPs) that similar morphology to native rotavirus. In order establish most favorable conditions for synthesis VLPs, we have compared kinetics 2/6/7-VLP two different cell lines: High Five propagated Excell 405 medium Spodoptera frugiperda 9 400 medium. The majority VLPs produced both...
We have previously reported the safety and immunogenicity 4 weeks after 2 doses of Clover coronavirus disease 2019 (COVID-19) vaccine candidate, SCB-2019, a stabilized prefusion form severe acute respiratory syndrome (SARS-CoV-2) spike protein (S-trimer). now report persistence antibodies up to 6 months vaccination, cross-neutralization titers against 3 variants concern (VoCs).In phase 1 study, adult (18-54 years age) elderly (55-75 volunteers received vaccinations 21 days apart with placebo...
We previously demonstrated the efficacy of COVID-19 vaccine candidate, SCB-2019, in adults SPECTRA phase 2/3 study. extended study to include 1278 healthy 12–17-year-old adolescents Belgium, Colombia, and Philippines who received either two doses SCB-2019 or placebo 21 days apart, assess immunogenicity as neutralizing antibodies against prototype SARS-CoV-2 variants concern, safety reactogenicity solicited unsolicited adverse events with a comparator group young (18–25 years). In...
Abstract Background We compared homologous and heterologous boosting in adults the Philippines primed with 2 or 3 doses of CoronaVac, recombinant protein vaccine, SCB-2019. Methods CoronaVac-immunized (18–72 years) received a full half dose SCB-2019 booster. assessed all neutralizing antibody (NAb) responses against prototype SARS-CoV-2 after 15 days NAb Delta Omicron variants subsets (30‒50 per arm). Participants recorded adverse events. Results In 2-dose CoronaVac-primed geometric mean...
Telomerase activity is up-regulated 1000-fold higher in human tonsil germinal center B cells compared to resting naive or memory cells, and telomerase expression can be re-activated vitro cells. To understand the mechanism(s) of regulation, quiescent cell from peripheral blood were activated with different combinations various stimuli. Cross-linking surface (s)IgD sIgM induced marked up-regulation enzymatic absence cellular proliferation. Low level cross-linkage molecules by soluble anti-IgM...
Zika virus (ZIKV) serological diagnostics are compromised in areas where dengue viruses (DENV) co-circulate because of their high levels protein sequence homology. Here, we describe the characterization a blockade-of-binding ELISA (Zika BOB) and microneutralization assay MN) for detection ZIKV nonstructural 1 (NS1)-specific antibodies neutralizing antibodies, respectively. BOB MN cutoffs were established as 10 100 endpoint titers, respectively, using samples collected pre- post-virologically...
The hemagglutination inhibition (HAI) assay is the most commonly used serology to detect antibodies from influenza vaccination or virus infection. This has been for decades but requires improved standardization of procedures provide meaningful data.