A5012341372- Cervical Cancer and HPV Research
- Genital Health and Disease
- Hepatitis B Virus Studies
- Reproductive tract infections research
- Colorectal and Anal Carcinomas
- Sex work and related issues
- Endometrial and Cervical Cancer Treatments
- Ovarian cancer diagnosis and treatment
- Cancer Immunotherapy and Biomarkers
- Cancer and Skin Lesions
- Biosensors and Analytical Detection
- Lymphoma Diagnosis and Treatment
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Herpesvirus Infections and Treatments
- Advanced biosensing and bioanalysis techniques
- SARS-CoV-2 detection and testing
- Radiomics and Machine Learning in Medical Imaging
- Milk Quality and Mastitis in Dairy Cows
- MRI in cancer diagnosis
- Molecular Biology Techniques and Applications
- T-cell and Retrovirus Studies
- HIV, Drug Use, Sexual Risk
- HIV/AIDS Research and Interventions
- Sarcoma Diagnosis and Treatment
- Cancer Diagnosis and Treatment
Administración Nacional de Laboratorios e Institutos de Salud
2011-2024
National Institutes of Health
2011-2013
Instituto de Efectividad Clínica y Sanitaria
2013
Due to the scarce data on prevalence of sexually transmitted infections (STIs) among male-to-female trans-sex workers (TSW) and male sex (MSW) in Argentina, present study aimed estimate incidence human immunodeficiency virus (HIV), HIV, hepatitis B (HBV), C (HCV), Treponema pallidum. Human papillomavirus (HPV) Chlamydia trachomatis were tested TSW.Two hundred seventy-three TSW 114 MSW recruited by nongovernmental organizations. HIV was estimated STARHS (serologic testing algorithm for recent...
The proportion of HPV16 and 18-associated cervical cancer (CC) appears rather constant worldwide (≥70%), but the relative importance other HR-HPV differs slightly by geographical region. Here, we studied HPV genotype distribution positive Latin American (LA) women histological grade, in a sub-cohort from ESTAMPA study; also explored association age-specific genotypes severe lesions. Cervical samples 1,252 participants (854 ≤CIN1, 121 CIN2, 194 CIN3 83 CC) were genotyped two PCRs-Reverse...
High-risk types of human papillomavirus (HPV) are strongly associated with cervical cancer (CC), and Chlamydia trachomatis (CT), the most frequent sexually transmitted bacterial infection (STBI) worldwide, seems to be a risk factor for HPV CC. It is also known that both agents more prevalent in vulnerable communities where lack adequate primary health care cause concern. The aim this work was determine impact CT infections women belonging an isolated aboriginal population (Pilaga community)...
Anal squamous cell carcinoma (ASCC) is a rare gastrointestinal malignancy associated with high-risk Human papillomavirus (HPV) infection. Despite improved outcomes in non-metastatic ASCC, definitive chemoradiotherapy constitutes the standard treatment for localized disease. Evidences predictive and prognostic biomarkers are limited. Here, we performed viral, immune, mutational characterization of 79 ASCC patients complete chemoradiotherapy. HPV-16 was detected 91% positive cases single...
Objectives: High-risk types of human papillomavirus (HPV) are strongly associated with cervical cancer (CC), and Chlamydia trachomatis (CT), the most frequent sexually transmitted bacterial infection (STBI) worldwide, seems to be a risk factor for HPV CC. It is also known that both agents more prevalent in vulnerable communities where lack adequate primary health care cause concern. The aim this work was determine impact CT infections women belonging an isolated aboriginal population (Pilaga...
To determine the frequency of human papillomavirus (HPV) types and to assess bacterial vaginosis (BV) possible associations with cervical infections in indigenous Paraguayan women Department Presidente Hayes.This study included 181 sexually active without lesions. HPV typing was performed by polymerase chain reaction primers PGMY 09/11 followed reverse line hybridization. BV diagnosed Nugent criteria using results from a Gram stain smear.Sixteen percent were positive for at least one high...
Abstract The incidence of cervical cancer in Paraguay is among the highest world. This study aimed to determine distribution human papillomavirus (HPV) genotypes Paraguayan women, according severity lesion. cross‐sectional included 207 women without a squamous intraepithelial lesion, 164 with low‐grade lesions, 74 high‐grade and 41 cancer. Type‐specific HPV was determined by polymerase chain reaction MY9/11 L1 GP5+/GP6+ primers, followed restriction fragment length polymorphism reverse line...
Abstract The metastasis status of pelvic lymph nodes (PLNs) seems to be a predictive factor survival. It was suggested that the presence HPV DNA and other biological markers in PLN may indicate sub clinical early metastasis. aim describe prevalence distribution patterns H‐ras mutations intra operatively obtained cervical tumors PLN. Thirty‐seven 61 node biopsies from 37 patients with cancer were selected. typing location performed by PCR/dot blot situ hybridization (ISH) respectively....
Abstract Persistent infection with high‐risk human papillomavirus (HPV) causes cervical preneoplasic lesions and invasive cancer. This study evaluated the prevalence distribution of HPV genotypes in exfoliated cells from Uruguayan women. Five hundred sixty‐eight specimens were examined by PCR using MY09/11 primer set, genotyped restriction enzyme digestion (RFLP). Some samples which remained undetermined reanalyzed PGMY combined reverse line blot hybridization. Overall, about 42% positive...
• HPV16/18 decreased by >93% in vaccinated sexually active Argentine girls. Detected reduction of HPV31 and 45 would add to the success immunization. No genotype replacement was observed. First HPV vaccination monitoring data reported from a Latin American country.
Abstract Background Sexually transmitted infections (STIs) are prevalent throughout the world and impose a significant burden on individual health public systems. Missed diagnosis late treatment of STIs can lead to serious complications such as infertility cervical cancer. Although sexually co-infections common, most commercial assays target one or few STIs. The HPV-STI ChapterDx Next Generation Sequencing (NGS) assay detects quantifies 29 HPVs 14 other in single-tube single-step PCR...
In 2011, Argentina launched a government-funded national Human papillomavirus (HPV) immunization program incorporating bivalent HPV vaccine, with 0–1–6-month schedule, for girls 11 years of age, born after January 2000. Monitoring the changes infection prevalence among young women has been proposed as an endpoint early assessment vaccination programs. However, data on at ages are very limited. The aim this work was to determine and type-specific distribution in sexually active 15–17-year-old...
Our aim was to evaluate the analytical and clinical performance of SARS-CoV-2 molecular detection kits used in Argentina. Nine real-time reverse-transcription polymerase chain reaction (RT-qPCR) three loop-mediated isothermal amplification (RT-LAMP) assays were evaluated using World Health Organization (WHO) recommended test as reference method. A secondary standard calibrated for E, N RdRp genes against Pan American Organization—World Organization—International Standard calculate limit...
Sensitive and specific genotyping of human papillomaviruses (HPVs) is critical for the surveillance monitoring vaccine effectiveness. Here, HPV genotypes were identified in 137 cervical samples with different histology (79 ≤CIN1 58 CIN3+) using Nested-PCR followed by Next-Generation sequencing (NGS) relative proportions each genotype multiple infections computed. All had been previously genotyped PCR-Reverse Blotting Hybridization (PCR-RBH) thus allowing a concordance analysis between both...
Anal squamous cell carcinoma (ASCC) is a rare gastrointestinal malignancy linked to high-risk Human papillomavirus (HPV) infection, which develops from precursor lesions like Low-Grade Squamous Intraepithelial Lesions (LGSIL) and High-Grade (HGSIL). ASCC incidence varies across populations, posing increased risk for People Living with HIV (PLWH). Our investigation focused on transcriptomic metatranscriptomic changes (SILs) ASCC. Metatranscriptomic analysis highlighted specific bacterial...
En Paraguay la incidencia de cáncer cuello uterino (CCU) es superior a las observadas en otros paÃses región. El agente etiológico asociado al CCU el virus papiloma humano (VPH), esencialmente tipos alto riesgo oncogénicos. objetivo describir aspectos epidemiológicos infección genital por (VPH-AR) mujeres 25 64 años que consultaron servicios PatologÃa Cervical del MSPyBS, mayo diciembre 2013. Se utilizó Cobas 4800 HPV Test (Roche) permite detección individual VPH-16 y VPH-18 un...