A5038716360- Supramolecular Self-Assembly in Materials
- Alzheimer's disease research and treatments
- Lipid Membrane Structure and Behavior
- Bacterial Genetics and Biotechnology
- Microfluidic and Capillary Electrophoresis Applications
- Nanocluster Synthesis and Applications
- Polydiacetylene-based materials and applications
- Magnetic Properties and Synthesis of Ferrites
- Chemical Synthesis and Analysis
- Molecular spectroscopy and chirality
- Porphyrin and Phthalocyanine Chemistry
- Bacteriophages and microbial interactions
- Crystallization and Solubility Studies
- Biochemical and biochemical processes
- Multiferroics and related materials
- Surface Chemistry and Catalysis
- Redox biology and oxidative stress
- Antimicrobial Peptides and Activities
- Glycosylation and Glycoproteins Research
- Advancements in Battery Materials
- Neuroendocrine regulation and behavior
- Surfactants and Colloidal Systems
- Enzyme Catalysis and Immobilization
- Innovative Microfluidic and Catalytic Techniques Innovation
- Force Microscopy Techniques and Applications
University College London
2021-2024
National Physical Laboratory
2023-2024
London Centre for Nanotechnology
2021-2024
Aarhus University
2013-2022
Aarhus School of Architecture
2014-2019
Sino-Danish Centre for Education and Research
2019
University of Cambridge
2017-2018
Center for NanoScience
2018
National Center for Nanoscience and Technology
2014-2015
Gram-negative bacteria are surrounded by a protective outer membrane (OM) with phospholipids in its inner leaflet and lipopolysaccharides (LPS) leaflet. The OM is also populated many β-barrel outer-membrane proteins (OMPs), some of which have been shown to cluster into supramolecular assemblies. However, it remains unknown how abundant OMPs organized across the entire bacterial surface this relates lipids membrane. Here, we reveal from molecular cellular length scales, using atomic force...
A selective antimicrobial strategy based on triggering of pore-forming toxin, which is secreted by infective bacteria, was designed to fight <italic>Staphylococcus aureus</italic>.
Copper ferrite (CuFe2O4) magnetic hollow fibers were prepared by applying an organic sol-thermal decomposition method, and silver nanoparticles subsequently loaded on the calcination. The Ag–CuFe2O4 exhibited excellent antibacterial efficacy against four different bacteria (E. coli, S. typhi, aureus V. parahaemolyticus) with consistent results. Typical ferromagnetism behavior from enables their feasible recyclability.
Mechanical properties of amyloid-like fibrils are influenced by the secondary structure: for instance, a higher amount ordered β-sheets contributes to enhance rigidity while presence α-helices appears soften nanostructure reducing young's modulus.
Amyloid nanostructures are originated from protein misfolding and aberrant aggregation, which is associated with the pathogenesis of many types degenerative diseases, such as Alzheimer's disease (AD), Parkinson's (PD) Huntington's disease. The secondary conformation peptides a fundamental importance for aggregation toxicity amyloid peptides. In this work, Aβ25-35, fragment β(1-42) (Aβ42), was selected to investigate correlation between structures fibrils. Furthermore, each assemblies show...
A wide variety of inorganic nanomaterials have been exploited so far for their great potential biological applications. Some these materials could be valid candidates to modulate the assembly amyloid peptides, which is relevant amyloid-related diseases. In this work, we reveal that a carbon nanomaterial can indeed peptides and, additionally, show modulating effect closely related dimensionality nanomaterials.
Aberrant assembly of the amyloid-β (Aβ) is responsible for development Alzheimer's disease, but can also be exploited to obtain highly functional biomaterials. The short Aβ fragment, KLVFF (Aβ16-20), crucial and considered an aggregation inhibitor. Here, we show that acetylation turns it into extremely fast self-assembling molecule, reaching macroscopic ( i.e., mm) size in seconds. We metastable self-assembly directed toward a crystalline or fibrillar phase simply through chemical...
Peptides enable the construction of a diversity one-dimensional (1D) and zero-dimensional (0D) nanostructures by molecular self-assembly. To date, it is great challenge to construct two-dimensional (2D) from peptides. Here we introduce an organic molecule tune amphiphilic-like peptide assembly form peptide–organic 2D nanopatch structure. The nanomechanical properties were explored quantitative imaging force control manipulation. patches are multilayers composed several domains, which can be...
The variation in the positions of hydrophobic residues within a peptide sequence can tune self-assembly.
Spatiotemporally controlled release of transforming growth factor β1 from electrospun biomimetic nanofibers realized optimal cell viability and myofibroblast differentiation capacity, which holds great potential in wound healing application.
Circular dichroism spectroscopy has become a powerful tool to characterise proteins and other biomolecules. For heterogeneous samples such as those present for interacting proteins, typically only average spectroscopic features can be resolved. Here we overcome this limitation by using free-flow microfluidic size separation in-line with synchrotron radiation circular resolve the secondary structure of each component model protein mixture containing monomers fibrils. To enable objective, have...
Hexagonal ferrites, also known as hexaferrites, are a very interesting class of materials with peculiar magnetic and electrical properties. Recently, several applications, both commercial technological, have been developed by employing such materials, in recording separation, storage devices, catalysts. In particular, one dimensional nanostructured promising since they exhibit physical properties that cannot be found the bulk. this work, nanocrystalline Sr1 − xBa x Er y Fe12 yO19 (x = ≤ 0.4)...
The charge of a peptide influences final assembled structures. It is important to consider not only global charge, but also local, such as that found on the terminal residues. This work investigates change self-assembly through selection different amino acid sequences and by varying local residues C- N-termini.
Abstract Circular dichroism (CD) is a versatile tool to investigate the secondary structure of proteins. Conventionally, CD signals in far‐UV region are primarily attributed peptide bond absorption; likewise aromatic residue analysis has typically only focussed on near‐UV absorption characteristics. However, crucial information that contained electronic transitions occurring not been fully exploited date, despite significant potential offered by such measurements yield window into protein...
Amyloid-like cysteine-rich peptide fibers are investigated by AFM, SR-CD, TEM, Cryo-EM, SR-XPS and through gold-nanoparticle binding fibers. The positions of the Au nanoparticles along possibly correlated to position exposed cysteines on fiber surface.
Au–(dibenzo[<italic>fg</italic>,<italic>op</italic>]tetracene) porphyrazine has been synthesized on an Au(111) surface by using the precursor of dibenzo[<italic>fg</italic>,<italic>op</italic>]tetracene-5,6,12,13-tetracarbonitrile <italic>via</italic> cyclization cyanogroups with Au adatoms.
Generally, it is very difficult to detect the precise location of specific amino acids in a 3D self-assembled structure. F. Besenbacher, M. Dong, and co-workers reveal article 1400133 possibility find position cysteines exposed on cysteine-rich peptide fibers' surfaces. Gold nanoparticles (Au NPs) are employed as probe sense presence cysteines. In fact, positions Au NPs along fibers correlated fiber surface.
Complement proteins eliminate Gram-negative bacteria in serum via the formation of membrane attack complex (MAC) pores outer membrane. However, it remains unclear how poration leads to inner permeation and cell lysis. Using atomic force microscopy (AFM) on living Escherichia coli ( E. ), we probed MAC-induced changes envelope correlated these with subsequent death. Initially, survived despite hundreds MACs randomly distributed over surface. This was followed by larger-scale disruption...