A5068436889- Electrospun Nanofibers in Biomedical Applications
- Microbial Inactivation Methods
- Tissue Engineering and Regenerative Medicine
- 3D Printing in Biomedical Research
- Viral Infectious Diseases and Gene Expression in Insects
- Microfluidic and Bio-sensing Technologies
- Transgenic Plants and Applications
- Nerve injury and regeneration
- Congenital heart defects research
- CRISPR and Genetic Engineering
- Tendon Structure and Treatment
- Electrohydrodynamics and Fluid Dynamics
- Osteoarthritis Treatment and Mechanisms
- Gout, Hyperuricemia, Uric Acid
- Bone health and osteoporosis research
- Neuroscience and Neural Engineering
- Liver Disease Diagnosis and Treatment
- Pluripotent Stem Cells Research
- Hepatocellular Carcinoma Treatment and Prognosis
- Virus-based gene therapy research
- Cardiac Arrest and Resuscitation
- Respiratory Support and Mechanisms
- RNA Interference and Gene Delivery
- Plasma Applications and Diagnostics
- Brain Metastases and Treatment
University of South Florida
2024
Old Dominion University
2014-2024
Virginia Commonwealth University
2008-2013
Philips (Finland)
2011
The role of dominant transforming p53 in carcinogenesis is poorly understood. Our previous data suggested that aberrant proteins can enhance tumorigenesis and metastasis. Here, we examined potential mechanisms through which gain-of-function (GOF) induce motility. Cells expressing GOF -R175H, -R273H -D281G showed enhanced migration, was reversed by RNA interference (RNAi) or transactivation-deficient mutants. In cells with engineered endogenous mutants, migration reduced downregulation...
Accumulating data indicates that some cancer treatments can restore anticancer immunosurveillance through the induction of tumor immunogenic cell death (ICD). Nanosecond pulsed electric fields (nsPEF) have been shown to efficiently ablate melanoma tumors. In this study we investigated mechanisms and immunogenicity nsPEF-induced in B16F10 Our show vitro nsPEF (20-200, 200-ns pulses, 7 kV/cm, 2 Hz) caused a rapid dose-dependent which was not accompanied by caspase activation or PARP cleavage....
Collagen microfiber-based constructs have garnered considerable attention for ligament, tendon, and other soft tissue repairs, yet with limited clinical translation due to strength, biocompatibility, scalable manufacturing, challenges. Crosslinking collagen fibers improves mechanical properties; however, questions remain regarding optimal crosslinking chemistries, biodegradation, long-term stability, potential biotextile assemble at scale, limiting their usefulness. Here, we assessed over 50...
It is critically important to study head and neck squamous cell carcinoma tumorigenic mechanisms in order gain a better understanding of tumor development, progression, treatment. Unfortunately, representative three-dimensional (3D) model for these evaluations has yet be developed. The purpose this was replicate extracellular matrix (ECM) morphology utilizing electrospinning technology. First, the ECM evaluated by decellularizing samples analyzing fibrous structure scanning electron...
Electrospinning is often used to create scaffolding as a biomimetic of the extracellular matrix tissues. A frequent limitation this technique for three-dimensional tissue modeling poor cell infiltration throughout void volume scaffolds. Here, we generated low-temperature electrospun silk scaffolds and compared these with conventional in terms mechanical properties, volume, infiltration, viability, potential support mucosal models under culture conditions. Low-temperature supported fibroblast...
Gene electrotransfer is an effective approach for delivering plasmid DNA to a variety of tissues. Delivery molecules with electric pulses requires control the electrical parameters achieve delivery. Since discomfort or tissue damage may occur high applied voltage, reduction voltage while achieving desired expression be important improvement. One possible combine exogenously heat. Previous work performed in vitro demonstrated that increasing temperature before pulsing can enhance gene and...
Musculoskeletal tissue injuries, including volumetric muscle loss (VML), are commonplace and often lead to permanent disability deformation. Addressing this healthcare need, an advanced biomanufacturing platform, assembled cell-decorated collagen (AC-DC) bioprinting, is invented rapidly reproducibly create living biomaterial implants, using clinically relevant cells strong, microfluidic wet-extruded microfibers. Quantitative analysis shows that the directionality distribution of throughout...
Approximately 800, 000 surgical repairs are performed annually in the U.S. for debilitating injuries to ligaments and tendons of foot, ankle, knee, wrist, elbow shoulder, presenting a significant healthcare burden. To overcome current treatment shortcomings advance tendon ligament injuries, we have developed novel electrospun Tissue ENgineered Device (TEND), comprised type I collagen poly(D,L-lactide) (PDLLA) solubilized benign solvent, dimethyl sulfoxide (DMSO). TEND fiber alignment,...
Myocardial ischemia can damage heart muscle and reduce the heart's pumping efficiency. This study used an ischemic swine model to investigate potential for gene electro transfer of a plasmid encoding vascular endothelial growth factor improving perfusion and, thus, reducing cardiomyopathy following acute coronary syndrome. Plasmid expression was significantly greater in treated tissue compared injection alone. Higher also seen versus non-ischemic groups with parameters 20 Volts (p<0.03), 40...
Gene electrotransfer (GET) is a proven and valuable tool for in vivo gene delivery to variety of tissues such as skin, cardiac muscle, skeletal tumors, with controllable expression levels. Optimizing challenging hurdle preclinical studies, particularly skin indications, due differences electrical conductivity animal compared human dermis. Therefore, the goal this study was develop an ex model GET using recellularized dermis more closely mimic skin. Decellularized (DermACELL(®)) cultured...
Introduction. Current collagen fiber manufacturing methods for biomedical applications, such as electrospinning and extrusion, have had limited success in clinical translation, partially due to scalability, cost, complexity challenges. Here we explore an alternative, simplified scalable formation method, termed 'pneumatospinning,' generate submicron fibers from benign solvents. Methods results. Clinical grade type I atelocollagen calf corium was electrospun or pneumatospun sheets of aligned...
Gene therapy has a wide range of applications for various types pathologies. Viral methods gene delivery provide high levels expression but have safety concerns. Non-viral are largely known to lower expression. We aim address this issue by using plasmid DNA with smaller backbones increase when delivered non-viral methods. In study we compare between two vectors firefly luciferase encoding insert liposome complexes and electrotransfer as A 2-fold reduction in vector backbone size,...
Plasma-activated air (PAA) provides a noncontact DNA transfer platform. In the current study, PAA was used for delivery of plasmid in 3D human skin model, as well vivo. Delivery encoding luciferase to recellularized dermal constructs enhanced, resulting fourfold increase expression over 120 hours compared injection only (P < 0.05). green fluorescent protein (GFP) confirmed epidermal layers construct. vivo experiments were performed BALB/c mice, with target. exposure significantly enhanced...
A novel monopolar electroporation system and methodologies were developed for in vivo intended potential clinical applications such as gene therapy. We hypothesized that an asymmetric anode/cathode electrode applicator geometry could produce favorable electric fields electroporation, without the typical drawback associated with traditional needle parallel plate geometries. Three prototypes built tested delivery of reporter genes to skin a guinea pig model. Gene expression was evaluated terms...
Tendon injuries are a common athletic injury that have been increasing in prevalence. While there current clinical treatments for tendon injuries, they relatively long recovery times and often do not restore native function of the tendon. In study, gene electrotransfer (GET) parameters delivery to skin were optimized with monophasic biphasic pulses reporter effector genes towards optimizing underlying healing. Tissue twitching damage, as well expression distribution evaluated. Bioprinted...
Introduction Pathological calcium-containing crystals accumulating in the joints, synovial fluid, and soft tissues are noted most elderly patients, yet arthritic crystal formation remains idiopathic. Interestingly, elevated lactic acid bone erosion frequently among comorbidities clinical features of patients with highest incidence arthropathies. This work shows that particulates (modeling erosion) dissolve directly generate crystals, possibly presenting a mechanism for accumulation...
Damage from myocardial infarction (MI) and subsequent heart failure are serious public health concerns. Current clinical treatments therapies to treat MI damage largely do not address the regeneration of cardiomyocytes. In a previous study, we established that it is possible promote cardiac muscle with vascular endothelial growth factor B gene delivery directly ischemic myocardium. current study aim optimize cardioporation parameters increase expression efficiency by varying electrode...
Multiaxial filament winding is an additive manufacturing technique used extensively in large industrial and military yet unexplored for biomedical uses. This study adapts to biomanufacture scalable, strong, three-dimensional microfiber (3DMF) medical device implants potential orthopedic applications. Polylactide filaments were wound through a collagen 'resin' bath create organized, stable orthobiologic implants, which are sized common ligament (e.g. anterior cruciate ligament) tendon rotator...