A5064955606- Tissue Engineering and Regenerative Medicine
- Electrospun Nanofibers in Biomedical Applications
- Hernia repair and management
- Bone Tissue Engineering Materials
- Pelvic and Acetabular Injuries
- Congenital heart defects research
- Abdominal Surgery and Complications
- Mesenchymal stem cell research
- Hereditary Neurological Disorders
- Additive Manufacturing and 3D Printing Technologies
- Xenotransplantation and immune response
- Botulinum Toxin and Related Neurological Disorders
- Periodontal Regeneration and Treatments
- Occupational exposure and asthma
- Advanced Sensor and Energy Harvesting Materials
- Dental Implant Techniques and Outcomes
- MicroRNA in disease regulation
- Intestinal and Peritoneal Adhesions
- Advanced biosensing and bioanalysis techniques
- Neurological disorders and treatments
Lifenet Health
2016
McGowan Institute for Regenerative Medicine
2010-2013
University of Pittsburgh
2010-2013
University of Nottingham
2007-2008
Surgical mesh devices composed of synthetic materials are commonly used for ventral hernia repair. These provide robust mechanical strength and quickly incorporated into host tissue; factors that contribute to reduced recurrence rates. However, such cause a foreign body response with the associated complications fibrosis patient discomfort. In contrast, surgical naturally occurring extracellular matrix (ECM) constructive tissue remodeling, but lack materials. A method applying porcine dermal...
Smooth muscle cells (SMCs) are typically used as a cell source for the reconstruction of hollow organs by conventional tissue engineering techniques. However, necessity and advantage use tissue-specific SMCs unknown. The present study investigated phenotypic changes that occur following isolation in vitro expansion rat SMC populations isolated from three different tissues: aorta, oesophagus urinary bladder. rSMCs were enzymatic dispersion expanded culture techniques, yielding microscopically...
Biologic scaffold materials composed of extracellular matrix (ECM) have been shown to promote the formation site-specific, functional, host tissue following placement in a number preclinical and clinical studies. Endotoxin contamination biomaterials is thought result deleterious immune responses that may affect remodeling outcome when present significant quantities. However, exact amount endotoxin within or upon an ECM-based biologic required elicit adverse effects recipients currently...
The stable culture of myogenic cells and their differentiation into myotubes in vitro is often hindered by the mechanical destabilisation spontaneously contractile neotissue formed, resulting complete loss differentiating myotubes. Electrospun, parallel aligned nylon 6/6 microfibre arrays were use successfully for C2C12 myoblasts to form mechanically stable, orientated vitro. Myoblasts adhered strongly fibre array, forming a compact cell sheath across entire aligning individual direction...
Current extracellular matrix (ECM) derived scaffolds offer promising regenerative responses in many settings, however some applications there may be a desire for more robust and long lasting mechanical properties. A biohybrid composite material that offers both strength bioactivity optimal healing towards native tissue behavior solution to this problem. regionally distinct biocomposite scaffold composed of biodegradable elastomer (poly(ester urethane)urea) porcine dermal ECM gel was...
Bone repair frequently requires time-consuming implant construction, particularly when using un-formed implants with poor handling properties. We therefore developed osteoinductive, micro-fibrous surface patterned demineralized bone matrix (DBM) fibers for engineering both defect-matched and general three-dimensional implants.Implant molds were filled human cortical there compressed lyophilized, forming mechanically strong shaped DBM scaffolds. Enzyme linked immunosorbent assays mass...
Therapeutic botulinum neurotoxin type A preparations have found an increasing number of clinical uses for a large variety neuromuscular disorders and dermatological conditions. The accurate determination potency in the application toxins is critical to ensuring efficacy safety, currently achieved by using lethal dose (LD50) assay mice. Ethical concerns operational constraints associated with this prompted development alternative systems that could potentially lead its replacement. As one...
Composite polypropylene-based surgical mesh materials including various synthetic polymers and naturally occurring biomaterials have been developed to ameliorate device-associated inflammatory response associated reduced compliance of pure polypropylene meshes. This study evaluated the histomorphologic three composite meshes, Revive™, a polycarbonate polyurethane reinforced monofilamentous scaffold, Assure™, scaffold with resorbable anti-adhesion layer lactide caprolactone copolymer,...
Introduction: Current limitations of in vitro tissue engineering include long culturing, the accompanied risk infection and required cost intensive infrastructures. Objective this study is creation an „off-the-shelf“ heart valve for vivo endothelialization. The concept based on high specific DNA-aptamers immobilized valves as capture molecules endothelial progenitor cells (EPCs) circulating blood stream.