THz radiation is increasingly used for diagnostics in medicine. As technology utilizing continues to develop rapidly, it becoming important consider its biological effects and establish safe exposure standards parameters. The paper presents data on the clinical status functional properties of anterior posterior structures eyes rabbits after irradiation at frequency 2.3 THz. Terahertz was generated Novosibirsk Free Electron Laser (NovoFEL) "Siberian Synchrotron Radiation Centre" (Budker...

Introduction: Immunosenescence alters TNF receptor expression (TNFR1 and TNFR2), contributing to chronic inflammation (inflamm-aging) age-related diseases. Polymorphisms in TNFRSF1A TNFRSF1B may influence expression; however, their role age-dependent modulation remains unclear. This study examines TNFR1/TNFR2 dynamics on T cells, B monocytes across different ages evaluates the impact of genetic polymorphisms. Methods: PBMCs from 150 donors (18–60 years) were isolated via density-gradient...

Immunotherapy using dendritic cell-based vaccination is a natural approach the capabilities and functions inherent in patient’s immune system to eliminate tumor cells. The development of cell technologies evolved as disorders differentiation function cancer were studied; some these are antigen presentation, priming cytotoxic T-lymphocytes induction antigen-specific responses. At initial stage technology development, it was necessary develop protocols for vitro generation functionally mature...

Abstract Experimental autoimmune encephalomyelitis (EAE)‐prone C57BL/6 mice are used as a model of human multiple sclerosis. We immunize with myelin oligodendrocyte glycoprotein (MOG), DNA–histone and DNA‐methylated bovine serum albumin (met‐BSA) complexes to reveal different characteristics EAE development including bone marrow lymphocyte proliferation differentiation profiles hematopoietic stem cells. Immunization MOG 35‐55 results in the acceleration development. Anti‐DNA antibodies...

Abstract Immunization of experimental autoimmune encephalomyelitis ( EAE )‐prone C57 BL /6 mice with MOG 35‐55 (a model used to study aspects human multiple sclerosis) is known lead the production various abzymes. The catalytic IgGs that can efficiently hydrolyse myelin basic protein MBP ), and DNA associated changes in profile differentiation level proliferation bone marrow haematopoietic stem cells HSC s). As simulates abzymes high DN ase activity, we compared effects immunization on...

<b><i>Background:</i></b> Expression levels of cytokine and growth factor receptors have been found to be important in the regulation their action. Tumor necrosis factor-α (TNFα) is actively involved inflammation processes atopic dermatitis (AD), but role TNFα membrane (TNFR) regulatory function AD remains unclear. <b><i>Aim:</i></b> We aimed determine associations parameters TNFRα expression on immunocompetent cells with disease severity...

Remission is the primary objective of treating juvenile idiopathic arthritis (JIA). It still debatable whether early intensive treatment superior in terms earlier achievement remission. The aim this study was to evaluate effectiveness etanercept+methotrexate (ETA+MTX) combination therapy versus step-up MTX monotherapy with ETA added refractory disease. A multi-centre, double-blind, randomized active polyarticular JIA patients treated either ETA+MTX (n = 35) or placebo+MTX 33) for up 24...

Background . Atrial fibrillation (AF) and heart failure (HF) are tightly interrelated. The concurrence of these pathologies can aggravate the pathological process. geographic ethnic characteristics patients may significantly affect efficacy different types therapy patients’ compliance. objective this study was to analyze how features course diseases management HF + AF influence clinical outcomes. Methods data 1,003 from first Russian register with chronic atrial (RIF-CHF) were analyzed....

Background: One of the mechanisms regulating biological activity tumor necrosis factor (TNF) in cells is co-expression TNFR1/TNFR2 receptors. A model with a differential level receptor expression required to evaluate contribution these mechanisms. Aim: The development cellular compare effects TNF on depending presence both receptors and TNFR2 alone. Methods: TNFR1 absence modifications ZR-75/1 K-562 cell lines were obtained by knockout. deletions was confirmed Sanger sequencing, membrane...

IL-1 β is involved in the induction and maintenance of chronic inflammation rheumatoid arthritis (RA). Its activity regulated induced by soluble membrane-bound receptors, respectively. The effectiveness cytokine depends not only on percentage receptor-positive cells an immunocompetent subset but also density receptor expression. objective this study was to investigate expression receptors (IL-1R1 IL-1R2) terms number per cell different subsets immune RA patients before after a course basic...

Background: Tumor necrosis factor (TNF)-α is an inflammatory cytokine, the biological effects of which are mediated by interaction with specific membrane-bound receptors. To assess TNF-α receptor (TNFR) expression, it important to estimate both number cells that carry these receptors and per cell, because cell fate depends on balance between TNFRI TNFRII signaling. Objective: The aim present study was develop optimized protocol level expression TNFRII, using QuantiBRITE PE calibration beads....

Background: The co-expression patterns of type 1 and 2 tumor necrosis factor (TNF)-α membrane receptors (TNFR1/TNFR2) are associated with the presence, stage, activity allergic diseases. aim this study was to assess expression levels dynamics TNFRs on immune cells associations between their severity bronchial asthma (BA). Methods: Patients severe (n = 8), moderate 10), mild 4) BA were enrolled. As a comparison group, data from 46 healthy volunteers (HV) accessed. Co-expression TNFR1/2...

Tumour necrosis factor-alpha (TNF-α) and its inhibitors are involved in both defence against tuberculosis (TB) damage to the host by TB. Notably, change receptor expression on cell density is a key mechanism regulation of biological properties cytokines.To study differences TNF-α (TNFR) patients with active pulmonary (aPTB) correlation parameters disease severity.TNFR1/2 levels peripheral blood mononuclear cells (PBMCs) from 45 aPTB 150 healthy controls were analysed flow cytometry using...

Introduction: Tumor necrosis factor (TNFα) is an important proinflammatory cytokine in rheumatoid arthritis (RA) immune processes. However, TNFα activity and functions may be regulated by soluble receptors, which act as decoys, number, density, co-expression of its membrane-bound receptors type 1 2 (TNFR1 TNFR2). The aim this study was to reveal associations between TNFR1/2 profile parameters RA disease indicators. Methods: PBMC were analyzed from 46 healthy donors 64 patients with using...

Both the steroid- and NSAID-sparing effects of biologics in juvenile idiopathic arthritis (JIA) treatment are key aspects dynamics patient's condition. The proper selection enables maximum effectiveness reduction dosage concomitant therapy. Our aim was to study therapy during etanercept (ETA) methotrexate (MTX) patients with JIA.This analysis included 215 JIA (63.3% females) showing sufficient response main One hundred received MTX as therapy, 24 ETA monotherapy, 91 þ combination were...

One of the mechanisms cellular dysfunction during chronization immune-system-mediated inflammatory diseases is a change in profile expression and co-expression receptors on cells. The aim this study was to compare patterns redistribution TNF (TNFRs) among patients with different durations rheumatoid arthritis (RA) or asthma. Subgroup analysis performed RA (n = 41) asthma 22) disease duration<10 years >10 30 comparable healthy individuals. TNFR1 TNFR2 assessed T cells, B monocytes,...

This research delves into the intricate landscape of tumor necrosis factor-alpha (TNF-α) signaling, a multi-functional cytokine known for its diverse cellular effects. Specifically, we investigate roles two TNF receptors, TNFR1 and TNFR2, in mediating TNF-α-induced transcriptional responses. Using human K562 cell lines with TNFR2 knockouts, explore changes gene expression patterns following TNF-α stimulation. Our findings reveal distinct profiles knockout cells, shedding light on unique...

To date, a large amount of retrospectively collected data about treatment neurosurgical pathology have been accumulated. Modern methods medical statistics are necessary for correct interpretation the data. The article purpose is to demonstrate application one modern methods, Propensity Score Matching (PSM), in neurosurgery. use PSM avoids misinterpretation and obviates errors planning further prospective studies. For past 10 years, number published international PSM-based studies has...

Aim. (1) To evaluate the efficiency of endovascular embolization brain arteriovenous malformations (AVMs) with seizure or hemorrhage in relation to radicalness and postoperative complication development (2) determine predictors complications after AVMs. Methods. In this retrospective study, we examined treatment 192 patients AVMs (seizure group; n = 85) (hemorrhage 107). All underwent total malformations, follow-up period was 12 months. The two different patient groups were identified,...

Modulating specific biological effects through the changes in cytokine receptors' expression level remains poorly understood. This study aimed to investigate influence of dose-dependent effect TNF on balance between proapoptotic and proliferation response depending parameters TNFR1/2 density.Tumor cell lines (HEp-2, K-562, MCF-7, ZR-75/1, MOLT-4, IM-9, Raji) were characterized for co-expression using flow cytometry studied reveal rhTNF cycle apoptosis parameters. The associations among...