A5083065227- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- Monoclonal and Polyclonal Antibodies Research
- Cytokine Signaling Pathways and Interactions
- Immune Response and Inflammation
- CAR-T cell therapy research
- T-cell and B-cell Immunology
- Rheumatoid Arthritis Research and Therapies
- Systemic Lupus Erythematosus Research
- Immunodeficiency and Autoimmune Disorders
- IL-33, ST2, and ILC Pathways
- RNA Interference and Gene Delivery
- Cell Adhesion Molecules Research
- Cancer Immunotherapy and Biomarkers
- Inflammasome and immune disorders
- Mycobacterium research and diagnosis
- Tuberculosis Research and Epidemiology
- Asthma and respiratory diseases
- Advanced Biosensing Techniques and Applications
- NF-κB Signaling Pathways
- Multiple Sclerosis Research Studies
- Atherosclerosis and Cardiovascular Diseases
- Dermatology and Skin Diseases
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cell death mechanisms and regulation
Research Institute of Fundamental and Clinical Immunology
2016-2025
Sechenov University
2023-2025
Siberian Branch of the Russian Academy of Sciences
2017-2021
Russian Academy of Sciences
2012-2015
Center of Molecular Immunology (Cuba)
2015
Academy of Medical Sciences
2008-2013
Institute of Immunology
2012
Introduction: Immunosenescence alters TNF receptor expression (TNFR1 and TNFR2), contributing to chronic inflammation (inflamm-aging) age-related diseases. Polymorphisms in TNFRSF1A TNFRSF1B may influence expression; however, their role age-dependent modulation remains unclear. This study examines TNFR1/TNFR2 dynamics on T cells, B monocytes across different ages evaluates the impact of genetic polymorphisms. Methods: PBMCs from 150 donors (18–60 years) were isolated via density-gradient...
IL‐18 has proinflammatory effects and participates in both innate adaptive cellular humoral immunity. A number of SNPs that influence production are found the gene promoter region. We investigated association at − 607 137 with level protein by PBMC from healthy donors Southwestern Siberia. The genetic distribution these site was established PCR. determined ELISA. Our results showed carrying allele 137C have lower levels spontaneous LPS‐stimulated production. In contrast, 607A significant...
Abstract Myelin oligodendrocyte glycoprotein ( MOG ) is an antigen of the myelin sheath, which may trigger immune cell responses and production auto‐antibodies in multiple sclerosis MS ). In this study, we used 35‐55 ‐induced experimental autoimmune encephalomyelitis EAE ), a model human , to assess catalytically active immunoglobulin G (IgG) antibodies or abzymes have been shown be present sera patients with several diseases. Here, show that IgGs from control C57 BL /6 mice are inactive....
Abstract Experimental autoimmune encephalomyelitis (EAE)‐prone C57BL/6 mice are used as a model of human multiple sclerosis. We immunize with myelin oligodendrocyte glycoprotein (MOG), DNA–histone and DNA‐methylated bovine serum albumin (met‐BSA) complexes to reveal different characteristics EAE development including bone marrow lymphocyte proliferation differentiation profiles hematopoietic stem cells. Immunization MOG 35‐55 results in the acceleration development. Anti‐DNA antibodies...
Minimal residual disease remaining after resection of primary tumors can lead to tumor recurrence and metastasis, increasing mortality morbidity rates among cancer patients. Thus, there is a need for new technologies recognition elimination single cells in patient's body radiation therapy, chemotherapy, or surgical resection. Effector CD8+ T cells, also commonly known as cytotoxic lymphocytes (CTLs), play key role antitumor cellular immunity and, when properly activated, are able effectively...
Abstract Immunization of experimental autoimmune encephalomyelitis ( EAE )‐prone C57 BL /6 mice with MOG 35‐55 (a model used to study aspects human multiple sclerosis) is known lead the production various abzymes. The catalytic IgGs that can efficiently hydrolyse myelin basic protein MBP ), and DNA associated changes in profile differentiation level proliferation bone marrow haematopoietic stem cells HSC s). As simulates abzymes high DN ase activity, we compared effects immunization on...
The level of TNF receptors on various cells immune system and its association with the gene polymorphism were investigated. Determining levels membrane-bound α peripheral blood mononuclear (PBMCs) was performed by flow cytometry using BD QuantiBRITE calibration particles. Soluble receptor (sTNFRs) determined ELISA genotyping PCR-RFLP. Homozygous TT individuals at SNP<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M1"><mml:mo>-</mml:mo><mml:mn>609</mml:mn></mml:math>G/T TNFRI...
The aim of the study was to evaluate potential contribution made by tumor necrosis factor (TNF) autoantibodies pathogenesis bronchial asthma (BA).We used affinity chromatography methods and a magnetic separation procedure purify human specific TNF. were as calibration material determine absolute content TNF using enzyme-linked immunosorbent assay (ELISA). levels soluble receptors determined ELISA commercial test kits.We demonstrated significant increases in sera patients with uncontrolled...
<b><i>Background:</i></b> Expression levels of cytokine and growth factor receptors have been found to be important in the regulation their action. Tumor necrosis factor-α (TNFα) is actively involved inflammation processes atopic dermatitis (AD), but role TNFα membrane (TNFR) regulatory function AD remains unclear. <b><i>Aim:</i></b> We aimed determine associations parameters TNFRα expression on immunocompetent cells with disease severity...
Interleukin–18 is one of the main cytokines, inducing production IFNg. It important factor anti–infectious and anti–tumor immunity. The review represent molecular–genetic biochemical characteristics IL–18. data about producers, structure receptor IL–18 binding protein, as well signal transduction in cell are considered. Besides that, immune effects discussed. (Med. Immu nol., 2005, vol.7, № 4, pp 355–364)
Background: One of the mechanisms regulating biological activity tumor necrosis factor (TNF) in cells is co-expression TNFR1/TNFR2 receptors. A model with a differential level receptor expression required to evaluate contribution these mechanisms. Aim: The development cellular compare effects TNF on depending presence both receptors and TNFR2 alone. Methods: TNFR1 absence modifications ZR-75/1 K-562 cell lines were obtained by knockout. deletions was confirmed Sanger sequencing, membrane...
Recent fundamental and clinical studies have confirmed the effectiveness of utilizing potential immune system to remove tumor cells disseminated in a patient's body. Cytotoxic T lymphocytes (CTLs) are considered main effectors cell-mediated antitumor immunity. Approaches based on antigen presentation CTLs by dendritic (DCs) currently being intensively studied, because DCs more efficient through their initiation strong specific responses than other types antigen-presenting cells. Today, it...
IL-1 β is involved in the induction and maintenance of chronic inflammation rheumatoid arthritis (RA). Its activity regulated induced by soluble membrane-bound receptors, respectively. The effectiveness cytokine depends not only on percentage receptor-positive cells an immunocompetent subset but also density receptor expression. objective this study was to investigate expression receptors (IL-1R1 IL-1R2) terms number per cell different subsets immune RA patients before after a course basic...
Background: Tumor necrosis factor (TNF)-α is an inflammatory cytokine, the biological effects of which are mediated by interaction with specific membrane-bound receptors. To assess TNF-α receptor (TNFR) expression, it important to estimate both number cells that carry these receptors and per cell, because cell fate depends on balance between TNFRI TNFRII signaling. Objective: The aim present study was develop optimized protocol level expression TNFRII, using QuantiBRITE PE calibration beads....
Background: The co-expression patterns of type 1 and 2 tumor necrosis factor (TNF)-α membrane receptors (TNFR1/TNFR2) are associated with the presence, stage, activity allergic diseases. aim this study was to assess expression levels dynamics TNFRs on immune cells associations between their severity bronchial asthma (BA). Methods: Patients severe (n = 8), moderate 10), mild 4) BA were enrolled. As a comparison group, data from 46 healthy volunteers (HV) accessed. Co-expression TNFR1/2...
Tumour necrosis factor-alpha (TNF-α) and its inhibitors are involved in both defence against tuberculosis (TB) damage to the host by TB. Notably, change receptor expression on cell density is a key mechanism regulation of biological properties cytokines.To study differences TNF-α (TNFR) patients with active pulmonary (aPTB) correlation parameters disease severity.TNFR1/2 levels peripheral blood mononuclear cells (PBMCs) from 45 aPTB 150 healthy controls were analysed flow cytometry using...
Introduction: Tumor necrosis factor (TNFα) is an important proinflammatory cytokine in rheumatoid arthritis (RA) immune processes. However, TNFα activity and functions may be regulated by soluble receptors, which act as decoys, number, density, co-expression of its membrane-bound receptors type 1 2 (TNFR1 TNFR2). The aim this study was to reveal associations between TNFR1/2 profile parameters RA disease indicators. Methods: PBMC were analyzed from 46 healthy donors 64 patients with using...
TCR-like chimeric antigen receptor (CAR-T) cell therapy has emerged as a game-changing strategy in cancer immunotherapy, offering broad spectrum of potential targets, particularly solid tumors containing intracellular antigens. In this study, we investigated the cytotoxicity and functional attributes vitro-generated T-lymphocytes, engineered with CAR precisely targeting testis MAGE-A4. Through viral transduction, T-cells were genetically modified to express co-cultured MAGE-A4-expressing...