Abstract There is evidence that interleukin −10(IL‐10) interferes with the costimulatory properties of antigen‐presenting cells and, thereby, inhibits their ability to induce T cell activation. To determine whether this effect might involve modulation expression accessory molecules, we analyzed by flow cytometry influence human IL‐10 on basal intercellular adhesion molecule 1 (ICAM‐1) as well interferon γ(IFN‐γ)‐induced up‐regulation ICAM‐1 and B7 at surface monocytes. inhibited both...

The use of microfluidic drops as microreactors hinges on the active control certain fundamental operations such droplet formation, transport, division and fusion. Recent work has demonstrated that local heating from a focused laser can apply thermocapillary force liquid interface sufficient to block advance in microchannel (C. N. Baroud, J.-P. Delville, F. Gallaire R. Wunenburger, Phys. Rev. E: Stat., Nonlinear, Soft Matter Phys., 2007, 75(4), 046302). Here, we demonstrate generality this...

Control over the synthesis of monodisperse silica particles up to mesoscopic scale is generally made difficult due intrinsic limitation submicrometric dimensions and secondary nucleation in seeded experiments. To investigate this issue overcome these difficulties, we have implemented single step processing by quantifying effects progressive addition a diluted tetraethyl orthosilicate solution ethanol on size monodispersity particles. Contrary grown polymerization, with 2 microm were...

Abstract Most of the immunosuppressive effects interleukin‐10 (IL‐10) are related to functional inhibition antigen‐presenting cells (APC). Herein, we investigate influence recombinant (r)IL‐10 on human dendritic (DC) purified from peripheral blood healthy volunteers. First, found that rIL‐10 inhibited in a dose‐dependent manner proliferative responses as well production IL‐2 and interferon‐γ (IFN‐γ) mixed lymphocyte reaction (MLR) between T DC. This effect could be attributed direct DC, DC...

Droplets are natural candidates for use as microfluidic reactors, if active control of their formation and transport can be achieved. We show here that localized heating from a laser block the motion water-oil interface, acting valve two-phase flows. A theoretical model is developed to explain forces on drop due thermocapillary flow, predicting scaling law favors miniaturization. Finally, we how forcing applied sorting drops, thus demonstrating it may integrated in complex droplet systems.

Sonodynamic therapy (SDT) is a promising therapeutic modality known for its non-invasiveness, temporal-spatial controllability, and deeper tissue penetration. However, the SDT treatment efficacy still hampered by scarcity of ideal sonosensitizers complex tumor microenvironment (TME). To address these challenges, sono-metabolic nano-composite (TiO2-Au@DON) using metabolic reprogramming prodrugs 6-Diazo-5-oxo-l-norleucine (DON) grafted on TiO2-Au Janus nanoparticles (NPs) fabricated. The...

Abstract We examined by flow cytometry the expression of lipopolysaccharide (LPS) receptor CD14 molecule on monocytes after addition LPS to human whole blood. Within 30 min LPSinduced an increase in monocyte expression, peaking between 1 and 3 h andfollowedby a slow decrease. Maximal anti‐CD14 monoclonal antibody binding sites was estimated as twofold basal value. This effect, already observed with very low concentrations (10 pg/ml), dose dependent. Protein synthesis not involved...

We used thermocapillary stresses locally induced by laser on flowing drops to build high throughput drop switchers and sorters for digital microfluidics. Since the is disconnected chip, method does not require dedicated micropatterning. show switching efficiencies of 100% velocities up 1.3cm∕s, demonstrate involved mechanism, apply sorting droplets different natures lab-on-a-chip applications.

Colloidal chemistry provides an assortment of synthetic tools for tuning the shape semiconductor nanocrystals. To fully exploit shape- and structure-dependent properties nanorods, high-precision control on growth design is essential. However, achieving this precision highly challenging due to high temperatures (>350 °C) short reaction times (<8 minutes) often required these reactions. In study, we performed first investigation impact temperature time CdS-shell CdSe/CdS quantum rods. Our...

This study reports the synthesis of monoclinic clinobisvanite BiVO4 crystals with well-defined {010} and {110} facets nanometer sizes through controlled reactant addition hydrothermal treatment. By adjustment Bi3+ precursor rate, nanoplates significantly reduced edge length thickness were obtained compared to conventional microplates. The formation process involves nucleation surfactant-coated tetragonal zircon nanocrystals, which aggregate into spheroids before being transformed plates. A...

We report on a new class of electromagnetically driven fluid interface instability. Using the optical radiation pressure cw laser to bend very soft near-critical liquid-liquid interface, we show that it becomes unstable for sufficiently large beam power $P$, leading formation stationary beam-centered liquid microjet. explore behavior instability onset by tuning softness with temperature and varying size exciting beam. The mechanism is experimentally demonstrated. It simply relies total...

We experimentally study the deformations of liquid-liquid interfaces induced by a high-intensity focused ultrasonic beam. quantitatively verify that small-amplitude transparent chloroform-water interface are well described theory Langevin acoustic radiation pressure, in both static and dynamic regimes. The large-amplitude depend on direction propagation beam qualitatively similar to those electromagnetic pressure.

The thinning dynamics of a liquid neck before break-up, as may happen when drop detaches from faucet or capillary, follows different rules and dynamic scaling laws depending on the importance inertia, viscous stresses, capillary forces. If now reaches dimensions comparable to thermally excited interfacial fluctuations, for nanojet break-up fragmentation annealed nanowires, these fluctuations should play dominant role according recent theory observations. Using near-critical interfaces, we...

Abstract By examining the rupture of fluid necks during droplet formation surfactant-laden liquids, we observe deviations from expected behaviour for pinch-off such necks. We suggest that these are due to presence a dynamic (time-varying) interfacial tension at minimum neck location and extract this quantity our measurements on variety systems. The effects should change process drastically. However, show simple ansatz, which incorporates temporal tension, allows us understand dynamics...

The deformation of a fluid-fluid interface due to the thermocapillary stress induced by continuous Gaussian laser wave is investigated analytically. We show that direction liquid strongly depends on viscosities and thicknesses involved layers. first investigate case an separating two different layers while second part dedicated thin film squeezed external same liquid. These results are predictive for applications fields where localized stresses used produce flows or deform interfaces in...

We report on the direct experimental observation of laser-induced flows in isotropic liquids that scatter light. use a droplet microemulsion two-phase regime, which behaves like binary mixture. Close to its critical consolute line, undergoes large refractive index fluctuations The radiation pressure laser beam is focused onto soft interface between two phases and induces cylindrical liquid jet continuously emits droplets. demonstrate this dripping phenomenon takes place as consequence steady...

We experimentally investigate the thermocapillary migration induced by local laser heating of advancing front a growing droplet confined in microfluidic channel. When implies an effective increase interfacial tension, behaves as ``soft door'' whose stiffness can be tuned via optical parameters (beam power and waist). The light-driven velocity droplet, which opposes basic flow, is characterized for different types fluid injection, either pressure or flow rate driven, various channel aspect...

We unveil the generation of universal morphologies fluid interfaces by radiation pressure whatever is nature wave, acoustic or optical. Experimental observations reveal interface deformations endowed with step-like features that are shown to result from interplay between wave propagation and shape interface. The results supported numerical simulations a quantitative interpretation based on waveguiding properties field provided.

When a fluid is heated by the absorption of continuous laser wave, density decreases in area. This induces pressure gradient that generates internal motion fluid. Due to mass conservation, convection eddies emerge sample. To investigate these laser-driven bulk flows at microscopic scale, we built setup perform temperature measurements with fluorescent-sensitive dye on one hand, and measured flow pattern different beam powers, using particle image velocimetry technique other hand. Temperature...

The formation of closed-compact multilamellar vesicles (referred to in the literature as "onion texture") obtained upon shearing lamellar phases is studied using small-angle light scattering and cross-polarized microscopy. By varying shear rate gamma;, gap cell D, smectic distance d, we show that: (i) this structure occurs homogeneously at a well-defined wave vector q(i), via strain-controlled process, (ii) value q(i) varies (dgamma;/D)(1/3). These results strongly suggest that may be...

Dissymmetric nano/microsized spheres are very appealing because controlled dissymmetry brings an additional degree of freedom for the synthesis a new generation materials with spatially separated chemical properties. We explore this aspect by extending to spherical surfaces application field lithographic techniques that was up now essentially limited planar and cylindrical substrates. The method proposed uses strongly focused laser beam generate dissymmetric coatings on microparticles...