In the yeast Saccharomyces cerevisiae, environmental stress conditions that damage cell wall lead to activation of so-called "compensatory mechanism," aimed at preserving integrity through a remodeling this extracellular matrix. Here we used DNA microarrays investigate molecular basis response two agents induce transient damage; namely Congo Red and Zymolyase. Treatment cells with these elicited up-regulation 132 101 genes respectively, main functional groups among them being involved in...

Yeast mitogen-activated protein kinase (MAPK) signaling pathways transduce external stimuli into cellular responses very precisely. The MAPKs Slt2/Mpk1 and Hog1 regulate transcriptional of adaptation to cell wall osmotic stresses, respectively. Unexpectedly, we observe that the activation a integrity (CWI) response damage caused by zymolyase (beta-1,3 glucanase) requires both HOG SLT2 pathways. Zymolyase activates Slt2 depends on Sho1 branch pathway under these conditions. Moreover,...

The adaptation of Saccharomyces cerevisiae to situations in which cell wall integrity is seriously compromised mainly involves the (CWI) pathway. However, a recent work ( Bermejo, C., Rodriguez, E., García, R., Rodríguez-Peña, J. M., Rodríguez de la Concepción, M. L., Rivas, Arias, P., Nombela, Posas, F., and Arroyo, (2008) Mol. Biol. Cell 19, 1113-1124 ) we have demonstrated co-participation high osmotic response (HOG) pathway ensure yeast survival stress mediated by zymolyase, hydrolyzes...

Solar Photovoltaics has become one of the cheapest sources electricity, with potential to expand further through distributed generation. PV self-consumption can empower electricity customers, transforming them into prosumers, but its success relies on appropriate regulations, especially in treatment surplus being net-metering and net-billing most common remuneration mechanisms. As number such systems increases, regulation needs evolve may affect profitability. The purpose this research is...

The fungal cell wall forms a compact network whose integrity is essential for morphology and viability. Thus, cells have evolved mechanisms to elicit adequate adaptive responses when (CWI) compromised. Functional genomic approaches provide unique opportunity globally characterize these mechanisms. To global perspective on CWI regulatory mechanisms, we developed chemical-genomic profiling of haploid mutant budding yeast systematically identify in parallel those genes required cope with...

Abstract Fungal cells trigger adaptive mechanisms to survive in situations that compromise cell wall integrity. We show here the global transcriptional response elicited by inhibition of synthesis β-1,3-glucan caspofungin, encompasses a set genes are dependent on Slt2, MAPK Cell Wall Integrity (CWI) pathway, and broad group regulated independently Slt2. Genes negatively cyclic AMP/Protein Kinase A (PKA) signaling pathway were overrepresented latter group. Moreover, stress mediated synthesis,...

In Saccharomyces cerevisiae, the transcriptional program triggered by cell wall stress is coordinated Slt2/Mpk1, mitogen-activated protein kinase (MAPK) of integrity (CWI) pathway, and mostly mediated transcription factor Rlm1. Here we show that SWI/SNF chromatin-remodeling complex plays a critical role in orchestrating response regulated swi/snf mutants drastically reduced expression stress-responsive genes hypersensitivity to wall-interfering compounds. On stress, binding RNA Pol II...

Abstract Background The yeast cell wall integrity mitogen-activated protein kinase (CWI-MAPK) pathway is the main regulator of adaptation responses to stress in yeast. Here, we adopt a genomic approach shed light on two aspects that are only partially understood, namely, characterization gene functional catalog associated with CWI activation and extent which MAPK correlates transcriptional outcomes. Results A systematic mutant deletion library was screened for constitutive CWI-related...

Activation of the yeast cell wall integrity (CWI) pathway induces an adaptive transcriptional programme that is largely dependent on transcription factor Rlm1 and mitogen-activated protein kinase (MAPK) Slt2. Upon stress, recruited to promoters RLM1 SLT2, exerts positive-feedback mechanisms expression both genes. MAPK Slt2 by stress not impaired in strains with individual blockade any two feedback pathways. Abrogation autoregulatory mechanism severely affects response elicited activation CWI...

The transcriptional response of Saccharomyces cerevisiae to cell wall stress is mainly mediated by the integrity (CWI) pathway through MAPK Slt2 and transcription factor Rlm1. Once activated, Rlm1 interacts with chromatin remodeling SWI/SNF complex which locally alters nucleosome positioning at target promoters. Here we show that SAGA plays along an important role for eliciting both early induction sustained gene expression upon stress. Gcn5 co-regulates together Swi3 majority CWI program,...

Cell wall stress in the model yeast Saccharomyces cerevisiae is known to trigger an adaptive transcriptional response. This response mediated by a specific MAPK cell integrity (CWI) signal transduction pathway and affects expression of many genes whose products are involved remodeling cellular envelope. damage detected mainly Wsc1 Mid2, which dominant sensors CWI pathway. Here, we first determined different stresses (Congo red, Caspofungin, Zymolyase) mid2Δ wsc1Δ mutant strains using DNA...

Abstract Opioid withdrawal signs, such as hyperalgesia, are manifestations of opioid use disorder that may contribute to seeking and taking. We have previously identified an association between dorsal raphe (DR) neurons the expression hyperalgesia during spontaneous heroin withdrawal. Here, we found chemogenetic inhibition DR decreased in male female C57/B6 mice. By neuroanatomy, three major subtypes expressing μ-opioid receptors (MOR) were activated withdrawal, those vesicular GABA...

The enormous amount of data available in public gene expression repositories such as Gene Expression Omnibus (GEO) offers an inestimable resource to explore programs across several organisms and conditions. This information can be used discover experiments that induce similar or opposite patterns a given query, which turn may lead the discovery new relationships among diseases, drugs pathways, well generation hypotheses. In this work, we present MARQ, web-based application allows researchers...

Abstract Post-transcriptional control of mRNA is a key event in the regulation gene expression. From yeast to human cells, P-bodies are cytoplasmic RNA-protein aggregates that play an essential role this process, particularly under stress conditions. In work, we show model Saccharomyces cerevisiae cell wall induces formation these structures. This effect dependent on multiple elements Cell Wall Integrity (CWI) MAPK signalling pathway, signal transduction cascade responsible for maintenance...

Within the field of Saccharomyces cerevisiae functional genomics, DNA microarrays have become a very useful tool to study genome-wide gene-expression changes under diverse experimental conditions. Here, design and production gene microarray, called ‘yeast cell wall chip’, specifically tailored investigate functions, is described. This array has been validated shown be address involvement in regulation response damage yeast. The advantages this which contains 390 genes, terms reproducibility,...

Atherosclerosis in symptomatic peripheral arterial disease affects wide portions of numerous arteries lower extremities. The resulting active inflammation a considerable amount tissue facilitates systemic detection via measurement inflammation-related variables. We reasoned that the combined assessment defense against oxidative stress, form paraoxonase-1 (PON1), and monocyte migration measured as circulating (C-C motif) ligand 2 (CCL2), may play role evaluation these patients. Plasma CCL2...

Abstract As a result of the relatively few available antifungals and increasing frequency resistance to them, development novel is increasingly important. The plant natural product poacic acid (PA) inhibits β‐1,3‐glucan synthesis in Saccharomyces cerevisiae has antifungal activity against wide range pathogens. However, mode action PA unclear. Here, we reveal that specifically binds β‐1,3‐glucan, its affinity for which ~30‐fold chitin. Besides effect on synthase activity, inhibited yeast...

Cellular mRNA levels, particularly under stress conditions, can be finely regulated by the coordinated action of transcription and degradation processes. Elements 5'-3' pathway, functionally associated with exonuclease Xrn1, bind to nuclear chromatin modulate gene transcription. Within this group are so-called decapping activators, including Pat1, Dhh1, Lsm1. In work, we have investigated role Pat1 in yeast adaptive transcriptional response cell wall stress. Thus, demonstrated that absence...

We report the sequence of a 20 300 bp DNA fragment from left arm Saccharomyces cerevisiae chromosome IV. This segment contains 13 complete open reading frames (ORFs) and part another ORF, altogether covering 84.2% entire sequence, five which correspond to previously characterized KIN28, MSS2, PHO2, POL3/CDC2 DUN1 genes. One putative protein, D2358p, shares considerable homology with an O-sialoglycoprotein endopeptidase Pasteurella haemolytica serotype A1. The product D2325 characteristic...