A5027113855- Cancer Genomics and Diagnostics
- RNA Interference and Gene Delivery
- AI in cancer detection
- Epigenetics and DNA Methylation
- Molecular Biology Techniques and Applications
- Gestational Trophoblastic Disease Studies
- Autism Spectrum Disorder Research
- Plant Virus Research Studies
- Child Nutrition and Feeding Issues
- Organ Transplantation Techniques and Outcomes
- Histone Deacetylase Inhibitors Research
- Mosquito-borne diseases and control
- Lysosomal Storage Disorders Research
- MicroRNA in disease regulation
- Nanoparticle-Based Drug Delivery
- Liver physiology and pathology
- Tissue Engineering and Regenerative Medicine
- Virus-based gene therapy research
- Cancer-related gene regulation
- CRISPR and Genetic Engineering
- Prostate Cancer Treatment and Research
- Viral Infections and Vectors
Cornell University
2024
Weill Cornell Medicine
2024
Johns Hopkins University
2017-2019
National Center for Advancing Translational Sciences
2018
National Institutes of Health
2018
Johns Hopkins Medicine
2017
The re-emergence of Zika virus (ZIKV) and Ebola (EBOV) poses serious continued threats to the global public health. Effective therapeutics for these maladies is an unmet need. Here, we show that emetine, anti-protozoal agent, potently inhibits ZIKV EBOV infection with a low nanomolar half maximal inhibitory concentration (IC50) in vitro potent activity vivo. Two mechanisms action emetine are identified: inhibition NS5 polymerase disruption lysosomal function. Emetine also entry. Cephaeline,...
Tay-Sachs disease (TSD) is a rare neurodegenerative disorder caused by autosomal recessive mutations in the HEXA gene on chromosome 15 that encodes β-hexosaminidase. Deficiency results accumulation of GM2 ganglioside, glycosphingolipid, lysosomes. Currently, there no effective treatment for TSD. We generated induced pluripotent stem cells (iPSCs) from two TSD patient dermal fibroblast lines and further differentiated them into neural (NSCs). The exhibited phenotype lysosomal lipid...
The patterns by which primary tumors spread to metastatic sites remain poorly understood. Here, we define of seeding in prostate cancer using a novel injection-based mouse model-EvoCaP (Evolution Cancer the Prostate), featuring aggressive bone, liver, lungs, and lymph nodes. To migration histories between sites, used our EvoTraceR pipeline track distinct tumor clones containing recordable barcodes. We detected widespread intratumoral heterogeneity from seeding, with few clonal populations...
Heterogeneous response to chemotherapy is a major issue for the treatment of cancer. For most gynecologic cancers including ovarian, cervical, and placental, list available small molecule therapies relatively compared options other cancers. While overall cancer mortality rates have decreased in United States as early diagnoses become more effective, ovarian still has low survival due lack effective options, drug resistance, late diagnosis. To understand chemotherapeutic diversity cancers, we...
To establish a rat model for evaluating the maturity of liver regeneration derived from associating partition and portal vein ligation staged hepatectomy (ALPPS).In present study, ALPPS, partial hepatecotmy (PHx), sham models were established initially, which validated by significant increase proliferative markers including Ki-67, proliferating cell nuclear antigen, cyclin D1. In setting accelerated proliferation in volume at second fifth day after characteristics newborn hepatocytes, as...
<p>Supplementary Figure S6. Barcode design and analysis. Supplementary S7. Analysis simulation barcode data to evaluate EvoTraceR performance. S8. Pipeline for detecting calling edits in barcode. S9. Temporal accumulation of vivo. S10. Eco-statistical analysis MMUS1495. S11. Metastatic trajectories EvoCaP mouse cohorts. S12. Organ diversity between mice. S13. Expansion platform vitro.</p>
<div>Abstract<p>The patterns by which primary tumors spread to metastatic sites remain poorly understood. Here, we define of seeding in prostate cancer using a novel injection-based mouse model—<i>EvoCaP</i> (Evolution Cancer the Prostate), featuring aggressive bone, liver, lungs, and lymph nodes. To migration histories between sites, used our <i>EvoTraceR</i> pipeline track distinct tumor clones containing recordable barcodes. We detected widespread...
<p>Supplementary Figure S1. Validation of EvoCaP platform in vitro. Supplementary S2. BC10 exhibited robust editing efficiency S3. Technical markers to identify tumor cells are present organs. S4. Consistent bioluminescence and fluorescence imaging tissues from mice.</p>
<p>Supplementary Figure S5. Immunostaining of tissues excised from prostate tumor regions.</p>
Glioblastoma multiforme (GBM) is the most common and aggressive type of primary brain tumor. Although gene therapy has shown a powerful strategy for GBM treatment in preclinical studies, its effectiveness yet to be achieved humans due large part an inability achieve widespread distribution tumor selectivity. Direct infusion into tissue straightforward approach bypass blood barrier (BBB), which hurdle experienced by systemically administered therapeutics, including vectors. Specifically,...