A5102129207- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- RNA modifications and cancer
- Genomics and Chromatin Dynamics
- Plant biochemistry and biosynthesis
- Adipose Tissue and Metabolism
- Immune Cell Function and Interaction
- Uterine Myomas and Treatments
- Endometriosis Research and Treatment
- RNA Research and Splicing
- Renal Transplantation Outcomes and Treatments
- Pregnancy and Medication Impact
- Organ Donation and Transplantation
- Cocoa and Sweet Potato Agronomy
- T-cell and B-cell Immunology
- Renal and related cancers
- Single-cell and spatial transcriptomics
- Bioenergy crop production and management
- Hair Growth and Disorders
- Bacterial Genetics and Biotechnology
- Immune cells in cancer
- Cytokine Signaling Pathways and Interactions
- Natural product bioactivities and synthesis
- NF-κB Signaling Pathways
- Genetic Syndromes and Imprinting
Van Andel Institute
2021-2025
Michigan State University
2017-2023
Cancer Research And Biostatistics
2023
Biostats (United States)
2022
Emerald Group Publishing (United Kingdom)
2021
Université Paris 1 Panthéon-Sorbonne
2021
Université Paris Nanterre
2021
Universidad Nacional de La Plata
2021
Kingston University
2021
Sustainability Institute
2021
Sweetpotato [Ipomoea batatas (L.) Lam.] is a globally important staple food crop, especially for sub-Saharan Africa. Agronomic improvement of sweetpotato has lagged behind other major crops due to lack genomic and genetic resources inherent challenges in breeding heterozygous, clonally propagated polyploid. Here, we report the genome sequences its two diploid relatives, I. trifida triloba, show that these high-quality assemblies are robust references hexaploid sweetpotato. Comparative...
Environmental nutrient availability influences T cell metabolism, impacting function and shaping immune outcomes. Here, we identified ketone bodies (KBs)-including β-hydroxybutyrate (βOHB) acetoacetate (AcAc)-as essential fuels supporting CD8
The spatial organization of genes within plant genomes can drive evolution specialized metabolic pathways. Terpenoids are important metabolites in plants with diverse adaptive functions that enable environmental interactions. Here, we report the genome assemblies Prunella vulgaris, Plectranthus barbatus, and Leonotis leonurus. We investigate origin subsequent a diterpenoid biosynthetic gene cluster (BGC) together other seven species Lamiaceae (mint) family. Based on core found BGCs all...
Deregulated inflammation is a critical feature driving the progression of tumors harboring mutations in liver kinase B1 (LKB1), yet mechanisms linking LKB1 to deregulated remain undefined. Here, we identify signaling by CREB-regulated transcription coactivator 2 (CRTC2) as an epigenetic driver inflammatory potential downstream loss. We demonstrate that sensitize both transformed and non-transformed cells diverse stimuli, promoting heightened cytokine chemokine production. loss triggers...
Coordination of cellular metabolism is essential for optimal T cell responses. Here, we identify cytosolic acetyl-CoA production as an metabolic node CD8 function in vivo. We show that responses to infection depend on derived from citrate via the enzyme ATP lyase (ACLY). However, ablation ACLY triggers alternative, acetate-dependent pathway mediated by acyl-CoA synthetase short-chain family member 2 (ACSS2). Mechanistically, acetate fuels both TCA cycle and production, impacting effector...
Abstract Recurrent gain-of-function mutations in the histone reader protein ENL have been identified Wilms tumor, most prevalent pediatric kidney cancer. However, their pathological significance development and tumorigenesis vivo remains elusive. Here, we generate mouse models mimicking tumor (ENL T ) show that heterozygous mutant expression Six2 + nephrogenic or Foxd1 stromal lineages leads to severe, lineage-specific defects, both resulting neonatal lethality. Six2-ENL kidneys display...
Abstract Mitragyna speciosa (kratom) produces numerous compounds with pharmaceutical properties including the production of bioactive monoterpene indole and oxindole alkaloids. Using a linked-read approach, 1,122,519,462 bp draft assembly M. “Rifat” was generated an N50 scaffold size 1,020,971 contig 70,448 that encodes 55,746 genes. Chromosome counting revealed is tetraploid base chromosome number 11, which further corroborated by orthology syntenic analysis genome. Analysis genes clusters...
Abstract Mutagenic purine–pyrimidine repeats can adopt the left-handed Z-DNA conformation. DNA breaks at potential sites lead to somatic mutations in cancer or germline that are transmitted next generation. It is not known whether any mechanism exists germ line control structure and repeats. Here we provide genetic, epigenomic biochemical evidence for existence of a biological process erases specifically cells mouse male foetus. We show previously uncharacterized zinc finger protein, ZBTB43,...
Uterine fibroids (leiomyomas) affect Black women disproportionately compared with of other races and ethnicities in terms prevalence, incidence, severity symptoms. The causes this racial disparity are essentially unknown. We hypothesized that myometria more susceptible to developing fibroids, we examined the transcriptomic DNA methylation profiles from White for comparison. Myometrial samples cluster by race both their transcriptome profiles, whereas fibroid only latter. More differentially...
The transformative events during early organismal development lay the foundation for body formation and long-term phenotype. rapid progression of limited material available present major barriers to studying these earliest stages development. Herein, we report an operationally simple RNA sequencing approach high-resolution, time-sensitive transcriptome analysis in (≤3 h) Drosophila embryos. This method does not require embryo staging but relies on single-embryo ordering along a developmental...
Switchgrass ( is a perennial native North American grass present in two ecotypes: upland, found primarily the northern range of switchgrass habitats, and lowland, largely southern reaches habitats. Previous studies focused on diversity panel switchgrass, so to expand our knowledge genetic broader set exome capture sequence data were generated for 632 additional, lowland individuals. In total, over 37 million single nucleotide polymorphisms (SNPs) identified 1.9 high-confidence SNPs obtained...
Sweet potato (Ipomoea batatas [L.] Lam.) is an important subsistence crop in Sub-Saharan Africa, yet as for many crops, yield can be severely impacted by drought stress. Understanding the genetic mechanisms that control tolerance facilitate development of drought-tolerant sweet cultivars. Here, we report expression profiling study using US-bred cultivar, Beauregard, and a Ugandan landrace, Tanzania, treated with polyethylene glycol (PEG) to simulate sampled at 24 48 hr after At each...
Antibody-secreting cells (ASCs) are key contributors to humoral immunity through immunoglobulin production and the potential be long-lived. ASC persistence has been recognized in autoimmune thymus (THY); however, only recently this population appreciated healthy THY tissue. We showed that young female was skewed toward higher of ASCs relative males. However, these differences disappeared with age. In both sexes, included Ki-67
Hypermethylation of CpG islands (CGI) is a common feature cancer cells and predominantly affects Polycomb-associated genomic regions. Elucidating the underlying mechanisms leading to DNA hypermethylation in human could help identify chemoprevention strategies. Here, we evaluated role Polycomb complexes 5-methylcytosine (5mC) oxidases protecting CGIs from methylation observed that four genes coding for components repressive complex 1 (PRC1) are downregulated tumors. Inactivation RYBP, key...
The Escherichia coli lac Operon is controlled by a regulatory system that has been the subject of intensive study for past fifty years. creates metabolic efficiency responding to levels environmental lactose. In absence lactose, LacI protein acts as repressor transcription from promoter. Transcription begins when lactose binds LacI, which results in expression three genes involved uptake and catabolism. promoter most commonly used field synthetic biology. Although it widely used, known have...
Chiococca alba (L.) Hitchc. (snowberry), a member of the Rubiaceae, has been used as folk remedy for range health issues including inflammation and rheumatism produces wealth specialized metabolites terpenes, alkaloids, flavonoids. We generated 558 Mb draft genome assembly snowberry which encodes 28,707 high-confidence genes. Comparative analyses with other angiosperm genomes revealed enrichment in lineage-specific genes involved metabolism. Synteny between Coffea canephora Pierre ex A....
Brown adipose tissue (BAT) has the ability to burn calories as heat. Utilizing BAT thermogenesis is thus an attractive way combat obesity. However, transcriptional network resulting in lipid synthesis oxidation shift during not completely understood. Here, we report regulation of two master regulators adipogenesis, peroxisome proliferator-activated receptor gamma (PPARγ) and CCAAT/enhancer-binding protein alpha (C/EBPα), acute cold stress BAT. We found PPARγ dissociates from DNA a fifth its...
DNA methylation is an epigenetic mark that plays important role in defining cancer phenotypes, with global hypomethylation and focal hypermethylation at CpG islands observed tumors. These marks can also be used to define tumor types provide avenue for biomarker identification. The homeobox gene class one has potential this use, as well other genes are Polycomb Repressive Complex 2 targets. To begin unravel relationship, we performed a pan-cancer analysis using sixteen Illumina HM450k array...
Chromatin architecture governs DNA accessibility and gene expression. Thus, any perturbations to chromatin can significantly alter expression programs promote disease. Prior studies demonstrate that every amino acid in a histone is functionally significant, even single substitution drive specific cancers. We previously observed naturally occurring H2B variants are dysregulated during the epithelial mesenchymal transition (EMT) bronchial cells. Naturally differ from canonical by only few...
Abstract Myometrial stem/progenitor cells (MyoSPCs) have been proposed as the of origin for uterine fibroids, but identity MyoSPC has not well established. We previously identified SUSD2 a possible marker, relatively poor enrichment in stem cell characteristics SUSD2+ over SUSD2- compelled us to find better markers. combined bulk RNA-seq SUSD2+/- with single identify markers MyoSPCs. observed seven distinct clusters within myometrium, vascular myocyte cluster most highly enriched and CRIP1...
The insulator model explains the workings of H19 and Igf2 imprinted domain in soma, where insulation promoter from its enhancers occurs by CTCF maternally inherited unmethylated chromosome but not paternally methylated allele. molecular mechanism that targets paternal methylation imprint establishment to imprinting control region (ICR) male germline is unknown. We tested function prospermatogonia-specific broad low-level transcription this process using mouse genetics. Paternal was abnormal...
Albright hereditary osteodystrophy (AHO) is a syndrome caused by inactivating mutations in the GNAS (guanine nucleotide-binding protein, alpha-stimulating) gene. Patients with AHO have short stature, obesity, brachydactyly and subcutaneous calcifications. can be associated pseudohypoparathyroidism type IA (PHP-IA) upregulation of parathyroid hormone, whereas pseudo-pseudohypoparathyroidism (PPHP), an endocrinopathy not present. We report case 5-month-old male infant who presented slowly...