A5046060822- Fungal and yeast genetics research
- Biofuel production and bioconversion
- RNA and protein synthesis mechanisms
- Genomics and Chromatin Dynamics
- Atherosclerosis and Cardiovascular Diseases
- Plant Disease Resistance and Genetics
- Cancer, Lipids, and Metabolism
- Protein Kinase Regulation and GTPase Signaling
- Retinoids in leukemia and cellular processes
- Gene expression and cancer classification
- Fluid Dynamics Simulations and Interactions
- Angiogenesis and VEGF in Cancer
- Genomics, phytochemicals, and oxidative stress
- Cell Adhesion Molecules Research
- Bioactive Compounds and Antitumor Agents
- Peptidase Inhibition and Analysis
- Peroxisome Proliferator-Activated Receptors
- Lung Cancer Research Studies
- Cancer, Hypoxia, and Metabolism
- Nanoparticles: synthesis and applications
- Chromatin Remodeling and Cancer
- Earthquake and Tsunami Effects
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Heavy Metal Exposure and Toxicity
- Technology Assessment and Management
Chungnam National University
2013-2023
Dankook University
2010
National Institute of Advanced Industrial Science and Technology
2010
Korea Research Institute of Bioscience and Biotechnology
2007-2010
Washington Hospital
1989
Eun Kyoung Choi, Hae Dong Kim, Jung Park, Seuk Young Song, Tien Thuy Phan, Miyoung Nam, Minjung Dong-Uk Kim and Kwang-Lae Hoe. Biomolecules & Therapeutics 2023;31:219-26. https://doi.org/10.4062/biomolther.2023.004
In small cell lung carcinoma, one of the short arms chromosome 3 is typically lost. To investigate in extrapulmonary we used DNA probes that detect restriction-fragment-length polymorphisms at loci on 3p. These were to study extracted from tumors and normal tissues and/or tumor lines five patients with cancer. Tumor four cancer retained heterozygosity Cytogenetic studies established these showed retention both 3. We conclude loss genetic material 3p observed not typical [J Natl Cancer Inst...
The fission yeast Schizosaccharomyces pombe is a model organism used widely to study various aspects of eukaryotic biology. A collection heterozygous diploid strains containing individual deletions in nearly all S. genes has been created using PCR based strategy. However, deletion some not possible this methodology. Here we use an efficient knockout strategy on plasmids that contain large regions homologous the target gene delete additional 29 genes. mutants now covers 99% open reading frames.
To identify target genes against silver nanoparticles (AgNPs), we screened a genome-wide gene deletion library of 4843 fission yeast heterozygous mutants covering 96% all protein encoding genes. A total 33 targets were identified by microarray and subsequent individual confirmation. The pattern AgNPs was more similar to those AgNO3 H2O2, followed Cd As. toxic effect on attributed the intracellular uptake AgNPs, release Ag+, leading generation reactive oxygen species (ROS). Next, focused top...
Vorinostat (VOR) has been reported to enhance the cytotoxic effects of doxorubicin (DOX) with fewer side because lower DOX dosage in breast cancer cells. In this study, we investigated novel mechanism underlying synergistic VOR and co-treatment cervical cells HeLa, CaSki SiHa Co-treatment at marginal doses led induction apoptosis through caspase-3 activation, poly (ADP-ribose) polymerase cleavage DNA micronuclei. Notably, growth inhibition induced by was attributed upregulation pro-apoptotic...
Low-density lipoprotein (LDL) induces cell proliferation in human aortic smooth muscle cells (hAoSMCs), which may be involved atherogenesis and intimal hyperplasia. Recent studies have demonstrated that Cl- channels are related to vessel induced by a variety of stimuli. In this study, we investigated potential role the signaling pathway LDL effects on hAoSMC with focus activation Erk1/2-PI3K/Akt subsequent upregulation Egr-1. channel blockers, DIDS, but neither NPPB nor Furosemide,...
Tamoxifen (TAM) is an anticancer drug used to treat estrogen receptor (ER)‒positive breast cancer. However, its ER-independent cytotoxic and antifungal activities have prompted debates on mechanism of action. To achieve a better understanding the action mechanisms TAM, we systematically identified TAM-sensitive genes through microarray screening heterozygous gene deletion library in fission yeast (Schizosaccharomyces pombe). Secondary confirmation was followed by spotting assay, finally...
We used a heterozygous gene deletion library of fission yeasts comprising all essential and non-essential genes for microarray screening target the antifungal terbinafine, which inhibits ergosterol synthesis via Erg1 enzyme. identified 14 strains corresponding to 10 [7 ribosomal-protein (RP) coding genes, spt7, spt20, elp2] 4 (tif302, rpl2501, rpl31, erg1). Expectedly, their erg1 mRNA protein levels had decreased compared control strain SP286. When we studied action mechanism using cognate...
Incorporation of unique barcodes into fission yeast gene deletion collections has enabled the identification functions by growth fitness analysis. For fine tuning, it is important to examine barcode sequences, because mutations arise during strain construction. Out 8,708 (4,354 strains) covering 88.5% all 4,919 open reading frames, 7,734 (88.8%) were validated as high-fidelity be inserted at correct positions Sanger sequencing. Sequence examination revealed that 1,039 (13.4%) deviated from...
Hepatitis C virus (HCV) non-structural 5A (NS5A) protein is associated with a wide variety of host signaling pathways by binding to C-terminal polyproline (PxxP) motifs various proteins. In this study, we used yeast two-hybrid analysis and GST pull-down assay screen novel NS5A interacting elucidate the site cellular focusing on recombinant human epidermal growth factor (rhEGF)-mediated ERK1/2 activation. Screening liver cDNA library revealed that h-prune, member DHH (Asp-His-His)...
Bar-code (tag) microarrays of yeast gene-deletion collections facilitate the systematic identification genes required for growth in any condition interest. Anti-sense strands amplified bar-codes hybridize with ~10,000 (5,000 each up- and down-tags) different kinds sense-strand probes on an array. In this study, we optimized hybridization processes array fission yeast. Compared to first version (11 µm, 100K) consisting three sectors probe pairs (perfect match mismatch), second 48K) could...