A5103052402- Fungal and yeast genetics research
- HIV Research and Treatment
- DNA Repair Mechanisms
- HIV/AIDS drug development and treatment
- Microtubule and mitosis dynamics
- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- Trace Elements in Health
- Animal Disease Management and Epidemiology
- Microbial Metabolic Engineering and Bioproduction
- Plant-Microbe Interactions and Immunity
- RNA and protein synthesis mechanisms
- Plant Virus Research Studies
- Bioinformatics and Genomic Networks
- Yeasts and Rust Fungi Studies
- Biochemical and Molecular Research
- Heat shock proteins research
- Fungal Biology and Applications
- Biofuel production and bioconversion
- CRISPR and Genetic Engineering
- Plant Gene Expression Analysis
- Polyamine Metabolism and Applications
- Protein Tyrosine Phosphatases
- Endoplasmic Reticulum Stress and Disease
- Genomics, phytochemicals, and oxidative stress
University of Debrecen
1997-2025
Institute of Animal Biochemistry and Genetics of the Slovak Academy of Sciences
2018-2022
Slovak Academy of Sciences
2018-2022
University of Maryland, Baltimore
2011-2021
Northwestern University
2004-2020
Max Perutz Labs
2010-2016
University of Vienna
2010-2016
University of Sussex
1998
The polyphyletic group of black fungi within the Ascomycota (Arthoniomycetes, Dothideomycetes, and Eurotiomycetes) is ubiquitous in natural anthropogenic habitats. Partly because their dark, melanin-based pigmentation, are resistant to stresses including UV- ionizing-radiation, heat desiccation, toxic metals, organic pollutants. Consequently, they amongst most stunning extremophiles poly-extreme-tolerant organisms on Earth. Even though ca. 60 fungal genomes have been sequenced date, [mostly...
Segregation of chromosomes during meiosis depends on separase cleavage Rec8, the meiosis-specific alpha-kleisin subunit cohesin. We mapped Rec8 phosphorylation sites by mass spectrometry and show that is required for proper chromosome disjunction meiosis. further fission yeast casein kinase 1 (CK1) delta/epsilon isoforms Hhp1 Hhp2 are full levels efficient removal at onset anaphase I. Our data consistent with model Hhp1/Hhp2-dependent separase-mediated
The fission yeast Schizosaccharomyces pombe is a model organism used widely to study various aspects of eukaryotic biology. A collection heterozygous diploid strains containing individual deletions in nearly all S. genes has been created using PCR based strategy. However, deletion some not possible this methodology. Here we use an efficient knockout strategy on plasmids that contain large regions homologous the target gene delete additional 29 genes. mutants now covers 99% open reading frames.
Several P1B-type ATPases are important Cd2+/Cu2+ pumps in Aspergillus species, and they tightly associated with the heavy metal stress tolerance of these ascomycetous fungi. To better understand roles two ATPases, nidulans CrpA pump (orthologue Candida albicans Crp1 pump) fumigatus PcaA Cd2+ Saccharomyces cerevisiae Pca1 pump), we have generated individual mutants characterized their susceptibilities. The deletion A. has led to increased sensitivity fungus stresses induced by Zn2+, Fe2+, or...
HIV-1 Vpr plays a pivotal role in viral pathogenesis and is preferentially targeted by the host immune system. In this report, we demonstrate that small heat shock protein, HSP27, exhibits Vpr-specific antiviral activity, as its expression specifically responsive to vpr gene increased levels of HSP27 inhibit Vpr-induced cell cycle G2 arrest killing. We further show overexpression reduces replication T-lymphocytes Vpr-dependent manner. Mechanistically, triggers through factor (HSF) 1, but...
Background HIV-1 protease (PR) is an essential viral enzyme. Its primary function to proteolyze the Gag-Pol polyprotein for production of enzymes and structural proteins maturation infectious particles. Increasing evidence suggests that PR cleaves host cellular proteins. However, nature PR-host protein interactions elusive. This study aimed develop a fission yeast (Schizosaccharomyces pombe) model system examine possible interaction with its potential impact on cell proliferation viability....
The human immunodeficiency virus type 1 (HIV-1) genome (~9 kb RNA) is flanked by two long terminal repeats (LTR) promoter regions with nine open reading frames, which encode Gag, Pol and Env polyproteins, four accessory proteins (Vpu, Vif, Vpr, Nef) regulatory (Rev, Tat). In this study, we carried out a genome-wide functional analysis of the HIV-1 in fission yeast (Schizosaccharomyces pombe).Each one genes was cloned expressed individually yeast. Subcellular localization each viral protein...
Many animal viral proteins, e.g., Vpr of HIV-1, disrupt host mitosis by directly interrupting the mitotic entry switch Wee1-Cdc25-Cdk1. However, it is unknown whether plant viruses may use this mechanism in their pathogenesis. Here, we report that 17K protein, encoded barley yellow dwarf and related poleroviruses, delays G
The reduction of chromosome number during meiosis is achieved by two successive rounds segregation after just single round DNA replication. To identify novel proteins required for the proper chromosomes meiosis, we analyzed consequences deleting Schizosaccharomyces pombe genes predicted to encode protein kinases that are not essential cell viability. We show Mph1, a member Mps1 family spindle assembly checkpoint kinases, prevent I homolog non-disjunction. also provide evidence function Spo4,...
ABSTRACT Human immunodeficiency virus type 1 (HIV-1) viral protein R (Vpr) exerts multiple effects on and host cellular activities during infection, including nuclear transport of the proviral integration complex, induction cell cycle G 2 arrest, death. In this report, we show that a fission yeast chaperone Hsp16 inhibits HIV-1 by suppressing these Vpr activities. This was identified through three independent genome-wide screens for multicopy suppressors each Consistent with properties heat...
Complementation of auxotrophic nutrient deficiencies in minimal media is widely used for selection exogenous gene introduction to fission yeast. However, only a limited number such markers are available. Antibiotic resistance good alternatives, but they typically work well complete rich medium not defined Edinburgh (EMM). It would be ideal if both the and antibiotic can together molecular genetic analysis. Here we describe use Zeocin Pombe glutamate (PMG) maintenance bleMX6 with LEU2 marker
To identify new proteins required for faithful meiotic chromosome segregation, we screened a Schizosaccharomyces pombe deletion mutant library and found that of the dbl2 gene led to missegregation chromosomes during meiosis. Analyses both live fixed cells showed dbl2Δ frequently failed segregate homologous opposite poles meiosis I. Removing Rec12 (Spo11 homolog) eliminate DNA double-strand breaks (DSBs) suppressed segregation defect in cells, indicating Dbl2 acts after initiation...
HIV-1 protease (PR) is an essential enzyme for viral production. Thus, PR inhibitors (PIs) are the most effective class of anti-HIV drugs. However, main challenge to successful use PI drugs in patient treatment emergence multidrug resistant PRs (mdrPRs). This study aimed develop a fission yeast cell-based system rapid testing new PIs that combat mdrPRs. Three mdrPRs were isolated from HIV-infected patients carried seven (M7PR), ten (M10PR) and eleven (M11PR) gene mutations, respectively....
Meiosis is the process by which haploid gametes are produced from diploid precursor cells. We used stable isotope labeling amino acids in cell culture (SILAC) to characterize meiotic proteome fission yeast Schizosaccharomyces pombe. compared relative levels of proteins extracted cells harvested around meiosis I with those II, and premeiotic S phase interval between divisions, when absent. Our datasets revealed peptides corresponding short open reading frames (sORFs) that have been previously...
Expression of the HIV-1 vpr gene in human and fission yeast cells displays multiple highly conserved activities, which include induction cell cycle G2 arrest death. We have previously characterized a heat shock protein 16 (Hsp16) that suppresses Vpr activities when it is overproduced yeast. Similar suppressive effects were observed hsp16 was overexpressed or context viral infection. In this study, we further molecular actions underlying effect Hsp16 on activities.We show Vpr-dependent...
Pre-mRNA splicing plays a fundamental role in securing protein diversity by generating multiple transcript isoforms from single gene. Recently, it has been shown that specific G-patch domain-containing proteins are critical cofactors involved the regulation of processes. In this study, using knock-out strategy, affinity purification and yeast-two-hybrid assay, we demonstrated spliceosome-associated Gpl1 fission yeast S. pombe mediates interactions between putative RNA helicase Gih35...
Two successive rounds of chromosome segregation following a single round DNA replication enable the production haploid gametes during meiosis. In fission yeast Schizosaccharomyces pombe, karyogamy is process where nuclei from 2 cells fuse to create diploid nucleus, which then undergoes meiosis produce 4 spores. By screening collection S. pombe deletion strains, we found that genes, mal3 and mto1, leads asci containing up 8 Here, show Mal3, member EB1 family conserved microtubule plus-end...
Successful combination antiretroviral therapies (cART) eliminate active replicating HIV-1, slow down disease progression, and prolong lives. However, cART effectiveness could be compromised by the emergence of viral multidrug resistance, suggesting need for new drug discoveries. The objective this study was to further demonstrate utility fission yeast cell-based systems that we developed previously discovery testing HIV protease (PR) inhibitors (PIs) against wild-type or multi-PI resistant...
Abstract During homologous recombination, Dbl2 protein is required for localisation of Fbh1, an F-box helicase that efficiently dismantles Rad51–DNA filaments. RNA-seq analysis dbl2Δ transcriptome showed the dbl2 deletion results in upregulation more than 500 loci Schizosaccharomyces pombe. Compared with no change expression, misregulated are closer to long terminal and tandem repeats. Furthermore, overlap antisense transcripts, retrotransposons, meiotic genes located subtelomeric regions. A...