A5085786245- Platelet Disorders and Treatments
- Fungal and yeast genetics research
- Microtubule and mitosis dynamics
- Antiplatelet Therapy and Cardiovascular Diseases
- DNA Repair Mechanisms
- Heavy Metal Exposure and Toxicity
- Cell Adhesion Molecules Research
- Heavy metals in environment
- Blood Coagulation and Thrombosis Mechanisms
- Fungal Biology and Applications
- Protein Kinase Regulation and GTPase Signaling
- Aluminum toxicity and tolerance in plants and animals
- Venous Thromboembolism Diagnosis and Management
- Heparin-Induced Thrombocytopenia and Thrombosis
- Phytochemicals and Antioxidant Activities
- Nanoparticles: synthesis and applications
- Plant Stress Responses and Tolerance
- Genomics and Chromatin Dynamics
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Potato Plant Research
- Fermentation and Sensory Analysis
- Peptidase Inhibition and Analysis
- Plant Reproductive Biology
- Yeasts and Rust Fungi Studies
- Plant responses to elevated CO2
Slovak University of Agriculture in Nitra
2013-2024
AgroBio
2018-2023
Max Perutz Labs
2010
University of Vienna
2010
University of Würzburg
2005-2009
Deutsche Forschungsgemeinschaft
2005-2008
Goethe University Frankfurt
2007
Bayer (Germany)
2007
Ruhr University Bochum
2006
Établissement Français du Sang
2006
Blood coagulation is thought to be initiated by plasma protease factor VIIa in complex with the membrane protein tissue factor. In contrast, XII (FXII)–mediated fibrin formation not believed play an important role for vivo. We used FXII-deficient mice study contributions of FXII thrombus Intravital fluorescence microscopy and blood flow measurements three distinct arterial beds revealed a severe defect stabilization platelet-rich occlusive thrombi. Although do experience spontaneous or...
Background— Ischemic stroke is a frequent and serious disease with limited treatment options. Platelets can adhere to hypoxic cerebral endothelial cells by binding of their glycoprotein (GP) Ib receptor von Willebrand factor. Exposure subendothelial matrix proteins further facilitates firm attachment platelets the vessel wall collagen GPVI receptor. In present study, we addressed pathogenic role GPIb, GPVI, aggregation GPIIb/IIIa in experimental mice. Methods Results— Complete blockade GPIbα...
Metastatic dissemination of cancer cells is the ultimate hallmark malignancy and accounts for approximately 90% human deaths. We investigated role acid sphingomyelinase (Asm) in hematogenous metastasis melanoma cells. Intravenous injection B16F10 into wild‐type mice resulted multiple lung metastases, while Asm‐deficient (Smpd1−/− mice) were protected from pulmonary tumor spread. Transplanting platelets reinstated metastasis. Likewise, MT/ret to spleen a mouse model spontaneous Human...
Segregation of chromosomes during meiosis depends on separase cleavage Rec8, the meiosis-specific alpha-kleisin subunit cohesin. We mapped Rec8 phosphorylation sites by mass spectrometry and show that is required for proper chromosome disjunction meiosis. further fission yeast casein kinase 1 (CK1) delta/epsilon isoforms Hhp1 Hhp2 are full levels efficient removal at onset anaphase I. Our data consistent with model Hhp1/Hhp2-dependent separase-mediated
Aspirin is effective in the therapy of cardiovascular diseases, because it causes acetylation cyclooxygenase 1 (COX-1) leading to irreversible inhibition platelets. Additional mechanisms can be suspected, patients treated with other platelet COX inhibitors such as indomethacin do not display an increased bleeding tendency observed for aspirin-treated patients. Recently, aspirin and anti-inflammatory drugs were shown induce shedding L-selectin neutrophils a metalloproteinase-dependent manner....
Abstract Blood coagulation factor XII (FXII, Hageman factor) is a plasma serine protease which autoactivated following contact with negatively charged surfaces in reaction involving kallikrein and high-molecular-weight kininogen (contact phase activation). Active FXII has the ability to initiate blood clotting via intrinsic pathway of inflammatory reactions kallikrein-kinin system. Here we have determined FXII-mediated bradykinin formation plasma. Western blotting analysis specific...
Objective— Collagen and thrombin are the strongest physiological platelet agonists, acting through different receptors, among which glycoprotein VI (GPVI) protease-activated respectively, essential ones. In mice, targeting of GPVI with monoclonal antibody JAQ1 induces depletion receptor from circulating platelets, resulting in abolished collagen responses long-lasting antithrombotic protection. Methods Results— Mice were treated JAQ1, early effects this treatment analyzed. addition to known...
Background and Objectives P-selectin has been implicated in important platelet functions. However, neither its role thrombus formation cardiovascular disorders nor suitability as a therapeutic target structure is entirely clear.Design Methods Platelet aggregation was assessed complementary vitro settings by measurements of static aggregation, standardized aggregometry dynamic flow chamber assays. Degradation aggregates also analyzed under conditions using video microscopy. In vivo, rolling...
Summary Platelets stably interact with collagen via glycoprotein (GP)VI and α2β1 integrin. With α2-null mice, we investigated the role of in thrombus formation stability vivo vitro. Using a FeCl3-induced thrombosis model, arteries from mice smaller thrombi were formed more embolization compared to vessels wild-type mice. Aspirin treatment causes similar effects, while thromboxane A2 analogue U46619 was borderline effective suppressing embolisation In vitro, perfusion blood over resulted that...
Toxicity of heavy metals to living organisms is a worldwide research topic. Although, much has been discovered about cadmium and nickel impact on biological systems, lot still remains unclear. We used inductively coupled plasma – optical emission spectroscopy address the question effect two different nickel, intracellular ion balance. Increase or decrease content several essential cations including Ca2+, Na+, K+, Mg2+, Cu2+, Fe3+ in yeast Schizosaccharomyces pombe was determined. Our results...
Cadmium has no known physiological function in the body; however, its adverse effects are associated with cancer and many types of organ system damage. Although much been shown about Cd toxicity, underlying mechanisms responses to organism remain unclear. In this study, role Tor1, a catalytic subunit target rapamycin complex 2 (TORC2), Cd-mediated on cell proliferation, antioxidant system, morphology, ionome balance was investigated eukaryotic model Schizosaccharomyces pombe. Surprisingly,...
Chromosome segregation during meiosis is a complex process, which leads to production of four haploid gametes from two precursor cells. Reversible phosphorylation proteins plays crucial role in this process. The Schizosaccharomyces pombe Prp4 an essential serine/threonine protein kinase, belongs the Clk/Sty family. To study meiosis, we analysed chromosome strain carrying conditional analog-sensitive allele kinase (prp4-as2). Our data show, that important as revealed by enhanced...
Acrylamide (AA), is a chemical with multiple industrial applications, however, it can be found in foods that are rich carbohydrates. Due to its genotoxic and cytotoxic effects, AA has been classified as potential carcinogen. With the use of spectrophotometry, ICP-OES, fluorescence spectroscopy, microscopy cell growth, metabolic activity, apoptosis, ROS production, MDA formation, CAT SOD ionome balance, chromosome segregation were determined Schizosaccharomyces pombe. caused growth activity...
Acrylamide (AA) a widely used industrial chemical is also formed during food processing by the Maillard reaction, which makes its exposure to humans almost unavoidable. In this study, we Schizosaccharomyces pombe as model organism investigate AA toxicity (10 or 20 mM concentration) in eukaryotes. S. pombe, delays cell growth causes oxidative stress enhancement of ROS production and triggers excitement antioxidant defence system resulting division arrest. Aronia fruit contains variety...
The Cornus mas L. fruit is well known for having a high presence of bioactive substances that include phenolic compounds, anthocyanins, vitamins, flavonoids, carotenoids, and ursolic acid. health-promoting effects those are mainly associated with their antimicrobial, anti-inflammatory, antioxidant properties. In this study, we investigated the role ethanolic extract on cell vitality model eukaryotic organism, yeast Schizosaccharomyces pombe. effect viability was determined by analyses growth...