A5091111257- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cell Adhesion Molecules Research
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Nanoplatforms for cancer theranostics
- Autoimmune Bullous Skin Diseases
- Platelet Disorders and Treatments
- Autoimmune and Inflammatory Disorders
- Immune cells in cancer
- Influenza Virus Research Studies
- Skin Diseases and Diabetes
- Blood disorders and treatments
- Psoriasis: Treatment and Pathogenesis
- S100 Proteins and Annexins
- Medicine and Dermatology Studies History
- Dermatology and Skin Diseases
- vaccines and immunoinformatics approaches
- Nanoparticle-Based Drug Delivery
- Advanced biosensing and bioanalysis techniques
- Immunotherapy and Immune Responses
- Long-Term Effects of COVID-19
- Graphene and Nanomaterials Applications
- RNA Interference and Gene Delivery
- Nanofabrication and Lithography Techniques
- Quantum Dots Synthesis And Properties
University Hospital Münster
2022-2025
University of Göttingen
2014-2024
Universitätsmedizin Göttingen
2013-2024
Universitätsmedizin Greifswald
2024
University of Münster
2021-2024
Robert Koch Institute
2017-2021
Biologie et Gestion des Risques en Agriculture
2020
University Medical Center
2020
Boston Children's Hospital
2013-2016
Harvard University
2015-2016
Neutrophilic granulocytes are able to release their own DNA as neutrophil extracellular traps (NETs) capture and eliminate pathogens. expulsion (NETosis) has also been documented for other cells organisms, thus highlighting the evolutionary conservation of this process. Moreover, dysregulated NETosis implicated in many diseases, including cancer inflammatory disorders. During NETosis, neutrophils undergo dynamic dramatic alterations cellular well sub-cellular morphology whose biophysical...
Aging promotes inflammation, a process contributing to fibrosis and decline in organ function. The release of neutrophil extracellular traps (NETs [NETosis]), orchestrated by peptidylarginine deiminase 4 (PAD4), damages organs acute inflammatory models. We determined that NETosis is more prevalent aged mice investigated the role PAD4/NETs age-related fibrosis. Reduction was seen hearts lungs PAD4-/- compared with wild-type (WT) mice. An increase left ventricular interstitial collagen...
Serotonin is an important neurotransmitter involved in various functions of the nervous, blood, and immune system. In general, detection small biomolecules such as serotonin real time with high spatial temporal resolution remains challenging conventional sensors methods. this work, we designed a near-infrared (nIR) fluorescent nanosensor (NIRSer) based on single-walled carbon nanotubes (SWCNTs) to image release from human blood platelets time. The consists nonbleaching SWCNT backbone, which...
The formation of Neutrophil Extracellular Traps (NETs) is an immune defense mechanism neutrophil granulocytes. Moreover, it also involved in the pathogenesis autoimmune, inflammatory and neoplastic diseases. For that reason, process NET subject intense ongoing research. In vitro approaches to quantify (NETosis) are commonly used involve stimulation with various activators such as phorbol 12-myristate 13-acetate (PMA), lipopolysaccharides (LPS) or calcium ionophores (CaI). However,...
Abstract Biochemical processes are fast and occur on small‐length scales, which makes them difficult to measure. Optical nanosensors based single‐wall carbon nanotubes (SWCNTs) able capture such dynamics. They fluoresce in the near‐infrared (NIR, 850–1700 nm) tissue transparency window emission wavelength depends their chirality. However, NIR imaging requires specialized indium gallium arsenide (InGaAs) cameras with a typically low resolution because quantum yield of normal Si‐based rapidly...
Neutrophils are sentinel immune cells with essential roles for antimicrobial defense. Most of our knowledge on neutrophil tissue navigation derived from wounding and infection models, whereas allergic conditions remained largely neglected. Here, we analyzed allergen-challenged mouse tissues discovered that degranulating mast (MCs) trap living neutrophils inside them. MCs release the attractant leukotriene B4 to re-route toward them, thus exploiting a chemotactic system normally use...
Notwithstanding their prominent presence in psoriatic skin, the functional role of neutrophilic granulocytes still remains somewhat enigmatic. Sparked by exciting scientific discoveries regarding neutrophil functions within last years, interest these short-lived cells innate immune system has been boosted recently. While it had known for some time that neutrophils produce and respond to a number inflammatory mediators, recent research linked with pathogenic IL-17, possibly conjunction...
Abstract Imaging of complex (biological) samples in the near-infrared (NIR) is beneficial due to reduced light scattering, absorption, phototoxicity, and autofluorescence. However, there are few NIR fluorescent materials known suitable for biomedical applications. Here we exfoliate layered pigment CaCuSi 4 O 10 (Egyptian Blue, EB) via ball milling facile tip sonication into nanosheets (EB-NS). The size EB-NS can be tailored diameters <20 nm heights down 1 nm. fluoresce at 910 fluorescence...
Integrins are transmembrane receptors that mediate cell-adhesion, signaling cascades and platelet-mediated blood clotting. Most integrins bind to the common short peptide Arg-Gly-Asp (RGD). The conformational freedom of RGD motif determines how strong which it binds. Here, we present a novel approach tune binding constants by confining motifs via noncovalent adsorption single-stranded DNA (ssDNA) anchors onto single-walled carbon nanotubes (SWCNTs). Semiconducting SWCNTs display fluorescence...
Neutrophils are the most abundant type of white blood cells. Upon stimulation, they able to decondense and release their chromatin as neutrophil extracellular traps (NETs). This process (NETosis) is part immune defense mechanisms but also plays an important role in many chronic inflammatory diseases such atherosclerosis, rheumatoid arthritis, diabetes, cancer. For this reason, much effort has been invested into understanding biochemical signaling pathways NETosis. However, impact mechanical...
Neutrophils are key effector cells of the innate immune system that respond to small signaling molecules, which coordinate and regulate responses. For a long time, similarities between neuronal have been discussed. Here, we show human neutrophils rapidly take up, package, traffic use catecholamine neurotransmitters such as dopamine or epinephrine via machinery known from neurons. Uptake release catecholamines well trafficking packaging into MPO/VMAT2-positive primary vesicles is visualized...
Hyperspectral imaging captures both spectral and spatial information from a sample but is intrinsically slow. The near infrared (NIR, > 800 nm) advantageous for applications because it falls into the tissue transparency window also contains vibrational overtone combination modes useful molecular fingerprinting. Here, fast hyperspectral NIR demonstrated using phasor transformation (HyperNIR). A liquid crystal variable retarder (LCVR) used tunable, wavelength-dependent sine- cosine-filtering...
Neutrophilic granulocytes play a fundamental role in cardiovascular disease. They interact with platelet aggregates via the integrin Mac-1 and receptor glycoprotein Ibα (GPIbα). In vivo, GPIbα presentation is highly variable under different physiological pathophysiological conditions. Here, we quantitatively determined conditions for neutrophil adhesion biomimetic vitro system, which allowed precise adjustment of spacings between human presented on nanoscale from 60 to 200 nm. Unlike most...
Cells use integrin receptors to adhere onto surfaces by binding ligands such as the arginine-glycine-aspartic acid (RGD) motif. Cancer cells make of this adhesion process, which has motivated development integrin-directed drugs. However, those drugs may exert paradoxical effects on tumor progression, raises question how function is governed in nanoscale. We have utilized precisely defined and tunable RGD ligand site densities spanning 1 order magnitude, i.e., 103 1145 sites/μm2, using...
Neutrophil Extracellular Traps (NETs) are produced by neutrophilic granulocytes and consist of decondensed chromatin decorated with antimicrobial peptides. They defend the organism against intruders released upon various stimuli including pathogens, mediators inflammation or chemical triggers. NET formation is also involved in inflammatory, cardiovascular, malignant diseases autoimmune disorders like rheumatoid arthritis, psoriasis systemic lupus erythematosus (SLE). In many SLE...
Inflammation is thought to play a critical role in the pathogenesis of placentation disorders such as recurrent miscarriages, growth restriction, and preeclampsia. Recently, neutrophil extracellular traps (NETs) have emerged potential mechanism for promoting inflammation both infectious noninfectious disorders. To investigate pathogenic NETs disorders, we studied model antiangiogenic factor-mediated pregnancy loss wild-type (WT) mice deficient peptidylarginine deiminase 4 (Padi4-/-) that are...