A5066596868- Supramolecular Self-Assembly in Materials
- Immunotherapy and Immune Responses
- RNA Interference and Gene Delivery
- Optical Coherence Tomography Applications
- Blood disorders and treatments
- Cell Adhesion Molecules Research
- Non-Invasive Vital Sign Monitoring
- Optical Imaging and Spectroscopy Techniques
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Silk-based biomaterials and applications
- Hemodynamic Monitoring and Therapy
- Cell Image Analysis Techniques
- Photoacoustic and Ultrasonic Imaging
- Advanced biosensing and bioanalysis techniques
- Fault Detection and Control Systems
- Antimicrobial Peptides and Activities
- Probabilistic and Robust Engineering Design
- Immune Response and Inflammation
- Advanced Fluorescence Microscopy Techniques
- Ovarian cancer diagnosis and treatment
- Lipid Membrane Structure and Behavior
- Medical Image Segmentation Techniques
- Monoclonal and Polyclonal Antibodies Research
- Protein Structure and Dynamics
- Cellular Mechanics and Interactions
University of Oklahoma
2020-2024
Erasmus MC
2016-2024
University of Göttingen
2018-2019
Universitätsmedizin Göttingen
2019
Bilkent University
2016-2018
Erasmus University Rotterdam
2017-2018
Ankara (Czechia)
2018
Rotterdam University of Applied Sciences
2017
National Nanotechnology Center
2016
National Nanotechnology Research Center
2016
Neutrophilic granulocytes are able to release their own DNA as neutrophil extracellular traps (NETs) capture and eliminate pathogens. expulsion (NETosis) has also been documented for other cells organisms, thus highlighting the evolutionary conservation of this process. Moreover, dysregulated NETosis implicated in many diseases, including cancer inflammatory disorders. During NETosis, neutrophils undergo dynamic dramatic alterations cellular well sub-cellular morphology whose biophysical...
Peptide amphiphiles (PAs) self-assemble into supramolecular nanofiber gels that provide a suitable environment for encapsulation of both hydrophobic and hydrophilic molecules. The PA have significant advantages controlled delivery applications due to their high capacity retain water, biocompatibility, biodegradability. In this study, we demonstrate injectable drug applications. Doxorubicin (Dox), as widely used chemotherapeutic breast cancer treatment, was encapsulated within the prepared at...
Neutrophils are the most abundant type of white blood cells. Upon stimulation, they able to decondense and release their chromatin as neutrophil extracellular traps (NETs). This process (NETosis) is part immune defense mechanisms but also plays an important role in many chronic inflammatory diseases such atherosclerosis, rheumatoid arthritis, diabetes, cancer. For this reason, much effort has been invested into understanding biochemical signaling pathways NETosis. However, impact mechanical...
Oligonucleotides are promising drug candidates due to the exceptionally high specificity they exhibit toward their target DNA and RNA sequences. However, poor pharmacokinetic pharmacodynamic properties, in conjunction with problems associated internalization by cells, necessitates delivery through specialized carrier systems for efficient therapy. Here, we investigate effects of morphology on cellular mechanisms oligonucleotides using self-assembled fibrous or spherical peptide...
The ability of dendritic cells to coordinate innate and adaptive immune responses makes them essential targets for vaccination strategies. Presentation specific antigens by is required the activation system against many pathogens tumors, nanoscale materials can be functionalized active targeting cells. In this work, we integrated an immunogenic, carbohydrate melanoma-associated antigen-mimetic GM3-lactone molecule into mannosylated peptide amphiphile nanofibers target through DC-SIGN...
The development of new biomaterials mimicking the neuronal extracellular matrix (ECM) requires signals for induction differentiation and regeneration.
The development of precision drugs for the selective treatment ovarian cancer will require targeting proliferative factors selectively expressed in tumors or unique physiological microenvironments specific tumors. Here, we report that oxysterol-binding protein (OSBP)-related 4 (ORP4) is a potential druggable target cells. ORP4 has limited expression normal tissues and was recently recognized to be cancer-specific driver cellular proliferation, including patient-isolated leukemias. We...
The three-dimensional (3D) tumor spheroid model is a critical tool for high-throughput ovarian cancer research and anticancer drug development
Immunogenic cell death (ICD) arises when cells are under stress, and their membranes damaged. They release damage-associated molecular patterns (DAMPs) that stimulate drive the type magnitude of immune response. In presence an antigen, DAMPs ride longevity efficacy antigen-specific immunity. Yet, no tool can induce controlled ICD with predictable results. A peptide-based tool, [II], is designed aggregates in causes membrane damage, generates on various types, hence act as adjuvant. An...
Optical coherence tomography (OCT) is an ideal imaging technique for noninvasive and longitudinal monitoring of multicellular tumor spheroids (MCTS). However, the internal structure features within MCTS from OCT images are still not fully utilized. In this study, we developed cross-statistical, cross-screening, composite-hyperparameter feature processing methods in conjunction with 12 machine learning models to assess changes structure. Our results indicated that effective combined...
The extracellular matrix (ECM) provides biochemical signals and structural support for cells, its functional imitation is a fundamental aspect of biomaterial design regenerative medicine applications. stimulation neural differentiation by laminin protein‐derived epitope in two‐dimensional (2D) three‐dimensional (3D) environments investigated. 3D gel system found to be superior 2D counterpart the induction differentiation, even absence crucial biological inducer nerve growth factor (NGF). In...
In CT-guided liver tumor ablation interventions, registration of a preoperative contrast-enhanced CT image to the intraoperative is hypothesized improve guidance. This highly challenging task due differences in patient poses and large deformations, therefore high errors are expected. this study, our objective develop method that enables users locally where fails, with minimal user interaction.The based on conventional nonrigid intensity-based framework, extended novel point-to-surface...
Percutaneous radio frequency ablation is a method for liver tumor treatment when conventional surgery not an option. It minimally invasive and may be performed under CT image guidance if the does give sufficient contrast on ultrasound images. For optimal guidance, registration of pre-operative contrast-enhanced to intra-operative hypothesized improve guidance. This highly challenging task due large differences in pose quality. In this study, we introduce semi-automated algorithm address...
The design and development of vaccines, which can induce cellular immunity, particularly CD8+ T cells hold great importance since these play crucial roles against cancers viral infections. Covalent conjugation antigen adjuvant molecules has been used for successful promotion immunogenicity in subunit vaccines; however, the stimulation T-cell responses by this approach so far limited. This study demonstrates a modular system based on noncovalent attachment biotinylated to hybrid nanofiber...
The regularization of retinal oxygen tension estimation was previously proposed with an assumption that phosphorescence intensity images are corrupted by additive Gaussian noise. Based on this assumption, a regularized least-squares estimate has been shown to be better than conventional estimation. However, is inconsistent the acquisition process acquired using intensified charge-coupled device camera. Almost entire governed natural aspects photons. Therefore, method based photon counting...
Abstract Neutrophils are the most abundant type of white blood cells. Upon stimulation, they able to decondense and release their chromatin as neutrophil extracellular traps (NETs). This process (NETosis) is part immune defense mechanisms but also plays an important role in many chronic inflammatory diseases such atherosclerosis, rheumatoid arthritis, diabetes cancer. For this reason, much effort has been invested into understanding biochemical signaling pathways NETosis. However, impact...
Abstract Utilizing multicellular aggregates (spheroids) for in vitro cancer research offers a physiologically relevant model that closely mirrors the intricate tumor microenvironment, capturing properties of solid tumors such as cell interactions and drug resistance. In this research, we investigated Peptide-Aggregation Induced Immunogenic Response (PAIIR), an innovative method employing engineered peptides designed specifically to induce immunogenic death (ICD). We contrasted PAIIR-induced...
Monitoring retinal oxygenation is of primary importance in detecting the presence some common eye diseases. To improve estimation oxygen tension vessels, regularized least-squares (RLS) method was shown to be very effective compared with conventional (LS) estimation. In this study, we propose an accelerated RLS for problem assessing vessels from phosphorescence lifetime images.In previous work, gradient descent algorithms were used find minimum cost function. This approach computationally...