A5016362953- Optical Coherence Tomography Applications
- Nanoplatforms for cancer theranostics
- Immune Cell Function and Interaction
- Cancer Immunotherapy and Biomarkers
- Photoacoustic and Ultrasonic Imaging
- Immunotherapy and Immune Responses
- Advanced Fluorescence Microscopy Techniques
- Spectroscopy Techniques in Biomedical and Chemical Research
- Cell Image Analysis Techniques
- interferon and immune responses
- CAR-T cell therapy research
- Photodynamic Therapy Research Studies
- Immune Response and Inflammation
- Graphene and Nanomaterials Applications
- Bacterial Infections and Vaccines
- Molecular Communication and Nanonetworks
- Cancer Research and Treatments
- Pancreatic and Hepatic Oncology Research
- Pneumonia and Respiratory Infections
- Nanoparticle-Based Drug Delivery
- Immune cells in cancer
- vaccines and immunoinformatics approaches
- Respiratory viral infections research
- Bladder and Urothelial Cancer Treatments
- 3D Printing in Biomedical Research
University of Oklahoma
2021-2024
The three-dimensional (3D) tumor spheroid model is a critical tool for high-throughput ovarian cancer research and anticancer drug development
Optical coherence tomography (OCT) is an ideal imaging technique for noninvasive and longitudinal monitoring of multicellular tumor spheroids (MCTS). However, the internal structure features within MCTS from OCT images are still not fully utilized. In this study, we developed cross-statistical, cross-screening, composite-hyperparameter feature processing methods in conjunction with 12 machine learning models to assess changes structure. Our results indicated that effective combined...
The tumor microenvironment (TME) promotes pro-tumor and anti-inflammatory metabolisms suppresses the host immune system. It prevents cells from fighting against cancer effectively, resulting in limited efficacy of many current treatment modalities. Different therapies aim to overcome immunosuppressive TME by combining various approaches synergize their effects for enhanced anti-tumor activity augmented stimulation Immunotherapy has become a major therapeutic strategy because it unleashes...
Abstract Immunotherapy has provided a new avenue to treat metastatic cancers, which result in ∼90% of cancer related deaths. However, current immunotherapies, such as immune checkpoint therapy (ICT), have met with limited success, primarily due tumor intrinsic and extrinsic factors that inhibit antitumor responses. To overcome the suppression microenvironment (TME) enhance tumoricidal activity ICT, phototherapy, particularly photothermal (PTT), combined nanomedicine become viable option. PTT...
Mucosal vaccinations for respiratory pathogens provide effective protection as they stimulate localized cellular and humoral immunities at the site of infection. Currently, major limitation intranasal vaccination is using adjuvants capable withstanding harsh environment imposed by mucosa. Herein, we describe efficacy a unique biopolymer, N-dihydrogalactochitosan (GC), nasal mucosal vaccine adjuvant against infections. Specifically, mixed GC with recombinant SARS-CoV-2 trimeric spike (S)...
Localized ablative immunotherapy (LAIT) combines targeted photothermal therapy (PTT) and to ablate the primary tumor site using laser irradiation while inducing systemic specific anti-tumor immune responses for treatment of metastatic cancers. The therapeutic efficiency LAIT synergized with nanomedicine has been proven be safe effective cancer treatment. Here, graphene oxide (GO) is used as a light-absorbing agent enhance generation heat caused by PTT increase its tumoricidal effect. GO also...
Pancreatic cancer is one of the lethal types due to its ability metastasize throughout body. Despite recent advances in pancreatic research, all current therapies have severe limitations treating metastatic cancer. Therefore, there a need for treatment regimen that can both break immunosuppressive nature tumor and control metastasis. We used novel localized ablative immunotherapy, combination interstitial photothermal therapy (iPTT) intratumoral administration immunostimulant, treat tumors...
N-dihydrogalactochitosan (GC) is an immune stimulant/adjuvant. Synthesized from chitosan and galactose, GC a new chemical entity that significantly enhances the immune-stimulating properties of its parental material, chitosan, making it promising therapeutic agent. When used in combination with antigenic stimulates innate adaptive antitumor antiviral immunities. However, mechanism has not been fully investigated. Herein we demonstrate drives type I IFN activation antigen-presenting cells...
Abstract N-dihydrogalactochitosan (GC) is developed for inducing immune responses. Synthesized from chitosan and galactose, GC a new chemical entity that significantly enhances the immune-stimulating properties of its parental material, chitosan, making it promising therapeutic agent. When used in combination with antigenic stimulates innate adaptive antitumor antiviral immunities. However, mechanism has not been fully investigated. Herein we demonstrate drives type I IFN production...
Abstract Mucosal vaccinations for respiratory pathogens provide effective protection as they stimulate localized cellular and humoral immunities at the site of infection. Currently, major limitation intranasal vaccination is using adjuvants capable withstanding harsh environment imposed by mucosa. Herein, we describe efficacy a novel biopolymer, N-dihydrogalactochitosan (GC), nasal mucosal vaccine adjuvant against infections. Specifically, COVID an example, mixed GC with recombinant...
Pancreatic tumors and melanoma are two types of cancers with high mortality. Monitoring the tumor microenvironment during growth plays an important role in studying property tumors. To study progression pancreatic vivo, we propose to use optical coherence tomography (OCT) monitor structure angiogenesis mouse a longitudinal manner. Our results demonstrate that OCT is promising tool longitudinally change structure, tissue distribution, microvasculature models. can serve as modality provide...
Three-dimensional (3D) cell culture models are developed as a promising platform to screen anticancer therapeutics and treatments. However, current imaging techniques cannot provide 3D structures of tumor spheroids in situ. In this study, we employed label-free noninvasive optical coherence tomography (OCT) for quantifying the spheroids. We imaged ovarian cancer with OVCAR-8 line over period 10 days 5,000 50,000 initial numbers. successfully reconstructed necrotic regions via intrinsic...
A novel biopolymer, N-dihydrogalactochitosan (GC), is developed for inducing immune responses. GC stimulates innate and adaptive antitumor antiviral immunities. In this study, we investigated the mechanism of GC-induced responses through in vitro vivo studies. We find that drives type I IFN production antigen presenting cells (APCs). Furthermore, alternative activation STING leading to inflammatory cell death enhances dendritic (DC) activation. vivo, induced a potent response upregulated...
Common two-dimensional (2D) models are ineffective in mimicking solid tumors. Additionally, fibrotic stroma is one of the major characteristics pancreatic tumors and it often missing vitro models. Therefore, there a need for better model to accurately mimic tumor detect progression fibrosis within model. Here, we utilized polarization-sensitive optical coherence tomography (PS-OCT) longitudinally collagen multicellular spheroids vitro. Spheroids were scanned by PS-OCT every two days, detected.
We utilized optical coherence tomography (OCT) to monitor the longitudinal progression of microenvironment and microvasculature pancreatic tumors before after photothermal therapy-induced immunotherapy in vivo. The primary implanted on mouse legs treated via were observed every three days for 36 days. intensity-based OCT structure, uniformity, texture, intrinsic attenuation contrast, vascular structures detected analyzed. Our result demonstrated that photothermal-induced could cause...
Pancreatic cancer is one of the deadliest types due to its ability metastasize throughout body. According Surveillance, Epidemiology, and End Results Program (SEER), overall 5-year relative survival rate for pancreatic 10.8% but once metastasizes, this reduces 3%. Despite recent advances in research, all current therapies have severe limitations treating cancer. One major challenge research lack an accurate model tumor microenvironment. The common two-dimensional (2D) models are ineffective...
Early assessment of sub-surface bladder tumor extension is challenging as there are no acceptable imaging modalities to determine three-dimensional (3D) extension. Current existing cystoscopy guided transurethral resection technique limited on direct visualization surface. In this paper, a multi-modal optical modality combing high-resolution coherence tomography (OCT) and depth-resolved high-sensitivity fluorescence laminar (FLOT) for structural molecular was developed. Bladder tissues from...