A5009041311- Immunotherapy and Immune Responses
- Cancer Immunotherapy and Biomarkers
- Immune Cell Function and Interaction
- Nanoplatforms for cancer theranostics
- T-cell and B-cell Immunology
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- CAR-T cell therapy research
- Circular RNAs in diseases
- Photodynamic Therapy Research Studies
- Microbial Natural Products and Biosynthesis
- Immune cells in cancer
- Phagocytosis and Immune Regulation
- Immune Response and Inflammation
- Radical Photochemical Reactions
- interferon and immune responses
- SARS-CoV-2 and COVID-19 Research
- Congenital heart defects research
- Electrocatalysts for Energy Conversion
- Fungal Biology and Applications
- Molecular Junctions and Nanostructures
- RNA modifications and cancer
- Graphene and Nanomaterials Applications
- vaccines and immunoinformatics approaches
- Cancer Research and Treatments
Oklahoma Medical Research Foundation
2019-2024
University of Oklahoma
2008-2024
University of Central Oklahoma
2018-2022
University of California, Berkeley
2017-2021
Pennsylvania State University
2020
The University of Texas Southwestern Medical Center
2011-2019
Berkeley College
2017
Southwestern Medical Center
2015
Fungi treated with DNA methyltransferase and histone deacetylase inhibitors exhibited natural product profiles enhanced chemical diversity demonstrating that small-molecule epigenetic modifiers are effective tools for rationally controlling the native expression of fungal biosynthetic pathways generating new biomolecules.
Combined phototherapy and immunotherapy demonstrates strong potential in the treatment of metastatic cancers. An upconversion nanoparticle (UCNP) based antigen-capturing nanoplatform is designed to synergize phototherapies immunotherapy. In particular, this constructed via self-assembly DSPE-PEG-maleimide indocyanine green (ICG) onto UCNPs, followed by loading photosensitizer rose bengal (RB). ICG significantly enhances RB-based photodynamic therapy efficiency UCNP/ICG/RB-mal upon activation...
A new fungal metabolite, nygerone (), featuring a unique 1-phenylpyridin-4(1H)-one core that had previously not been reported from any natural source, has obtained Aspergillus niger using chemical epigenetics methodology.
Chemical epigenetic manipulation of Penicillium citreonigrum led to profound changes in the secondary metabolite profile its guttate. While guttate from control cultures exhibited a relatively simple assemblage metabolites, collected treated with 50 μM 5-azacytidine (a DNA methyltransferase inhibitor) was highly enriched compounds representing at least three distinct biosynthetic families. The metabolites obtained fungus included six azaphilones (sclerotiorin (1), sclerotioramine (6),...
Background Physiological stress evokes rapid changes in both the innate and adaptive immune response. Immature αβ T cells developing thymus are particularly sensitive to stress, with infections and/or exposure lipopolysaccharide or glucocorticoids eliciting a apoptotic program. MicroRNAs class of small, non-coding RNAs that regulate global gene expression by targeting diverse mRNAs for degradation. We hypothesized subset thymically encoded microRNAs would be responsive modulate thymopoiesis....
TREX is a conserved multiprotein complex that necessary for efficient mRNA export to the cytoplasm. In Saccharomyces cerevisiae, additionally implicated in RNA quality control pathways, but it unclear whether this function mammalian cells. The Kaposi's sarcoma-associated herpesvirus (KSHV) ORF57 protein binds and recruits component REF/Aly viral mRNAs. Here, we demonstrate recruited KSHV noncoding polyadenylated nuclear (PAN) by ORF57. This recruitment correlates with ORF57-mediated...
Functional tuning of T cells based on their degree self-reactivity is established during positive selection in the thymus, although how differs for thymocytes with relatively low versus high unclear. In addition, preselection are highly sensitive to low-affinity ligands, but mechanism underlying enhanced cell receptor (TCR) sensitivity not fully understood. Here we show that murine experience briefer TCR signals and complete more slowly than those self-reactivity. Additionally, provide...
Abstract Background Metastatic breast cancer poses great challenge in treatment. N‐dihydrogalactochitosan (GC) is a novel immunoadjuvant that stimulates systemic immune responses when administered intratumourally following local tumour ablation. A combination of photothermal therapy (PTT) and GC, referred to as localized ablative immunotherapy (LAIT), extended animal survival generates an activated B cell phenotype MMTV‐PyMT mouse mammary microenvironment (TME). However, how T populations...
Rationale: B cells have emerged as key regulators in protective cancer immunity.However, the activation pathways induced during effective immunotherapy are not well understood.Methods: We used a novel localized ablative (LAIT), combining photothermal therapy (PTT) with intra-tumor delivery of immunostimulant N-dihydrogalactochitosan (GC), to treat mice bearing mouse mammary tumor virus-polyoma middle tumor-antigen (MMTV-PyMT).We single-cell RNA sequencing compare transcriptional changes by...
The tumor microenvironment (TME) promotes pro-tumor and anti-inflammatory metabolisms suppresses the host immune system. It prevents cells from fighting against cancer effectively, resulting in limited efficacy of many current treatment modalities. Different therapies aim to overcome immunosuppressive TME by combining various approaches synergize their effects for enhanced anti-tumor activity augmented stimulation Immunotherapy has become a major therapeutic strategy because it unleashes...
The thymus, an organ responsible for T cell development, is one of the more stress-sensitive tissues in body. Stress, form infections, radiation exposure, and steroids, impairs thymic epithelial (TEC) functions induces programmed death immature thymocytes. MicroRNAs are small noncoding RNAs involved tissue repair homeostasis, with several supporting development. We report that miR-205, epithelial-specific miR, maintains thymopoiesis following inflammatory perturbations. Thus, activation...
Autoreactive thymocytes are eliminated during negative selection in the thymus, a process important for establishing self-tolerance. Thymic phagocytes serve to remove dead thymocytes, but whether they play additional roles remains unclear. Here, using murine thymic slice model which undergo situ, we demonstrate that phagocytosis promotes selection, and provide evidence escape of autoreactive CD8 T cells periphery when thymus is impaired. We also show more efficient phagocyte presents...
Mycobacterial shuttle vectors contain dual origins of replication for growth in both Escherichia coli and mycobacteria. One such vector, pSUM36, was re-engineered high-level protein expression diverse bacterial species. The modified vector (pSUM-kan-MCS2) enabled green fluorescent E. coli, Mycobacterium smegmatis, M. avium at levels up to 50-fold higher than that detected with the parental which originally developed a lacZα promoter. This allowed easy visualization smegmatis infected...