A5081688603- Genetic Mapping and Diversity in Plants and Animals
- Plant Disease Resistance and Genetics
- Wheat and Barley Genetics and Pathology
- Chromosomal and Genetic Variations
- Plant Virus Research Studies
- Genetics and Plant Breeding
- Plant tissue culture and regeneration
- Plant Pathogens and Resistance
- Plant Pathogens and Fungal Diseases
- Plant Genetic and Mutation Studies
- Genetically Modified Organisms Research
- Powdery Mildew Fungal Diseases
- Genetic and Environmental Crop Studies
- Genomics and Phylogenetic Studies
- Fungal Plant Pathogen Control
- Bioenergy crop production and management
- Genetic and phenotypic traits in livestock
- Horticultural and Viticultural Research
- Agricultural pest management studies
- Weed Control and Herbicide Applications
- Genetic diversity and population structure
- Mycotoxins in Agriculture and Food
- Plant Reproductive Biology
- CRISPR and Genetic Engineering
- Crop Yield and Soil Fertility
Texas A&M University
2021-2024
Mitchell Institute
2023
Oregon State University
2004-2014
International Crops Research Institute for the Semi-Arid Tropics
2011-2014
International Crops Research Institute for the Semi-Arid Tropics
2011-2013
University of Minnesota
1992-2008
United States Department of Agriculture
2008
Agricultural Research Service
1997-2008
Illinois State University
2008
University of Missouri
2008
Accelerating crop improvement in sorghum, a staple food for people semiarid regions across the developing world, is key to ensuring global security context of climate change. To facilitate gene discovery and molecular breeding we have characterized ∼265,000 single nucleotide polymorphisms (SNPs) 971 worldwide accessions that adapted diverse agroclimatic conditions. Using this genome-wide SNP map, population structure with respect geographic origin morphological type identified patterns...
Abstract Background Pearl millet [ Pennisetum glaucum (L.) R. Br.] is a widely cultivated drought- and high-temperature tolerant C4 cereal grown under dryland, rainfed irrigated conditions in drought-prone regions of the tropics sub-tropics Africa, South Asia Americas. It considered an orphan crop with relatively few genomic genetic resources. This study was undertaken to increase EST-based microsatellite marker resources for this facilitate marker-assisted breeding. Results Newly developed...
ABSTRACT To improve knowledge of the prerequisites for meiotic chromosome segregation in higher eukaryotes, we analyzed spatial distribution a pair homologs before and during early prophase. Three-dimensional images fluorescence situ hybridization (FISH) were used to localize single diploid nuclei chromosome-addition line oat, oat-maize9b. The system provided robust assay pairing based on cytological colocalization FISH signals. Using triple labeling scheme simultaneous imaging chromatin,...
Abstract In maize (Zea mays L., 2n = 2x 20), map-based cloning and genome organization studies are often complicated because of the complexity genome. Maize chromosome addition lines hexaploid cultivated oat (Avena sativa 6x 42), where chromosomes can be individually manipulated, represent unique materials for analysis. particularly suitable dissection a single using radiation is an allohexaploid in which multiple copies basic provide buffering to chromosomal aberrations other mutations....
Abstract All 10 chromosomes of maize (Zea mays, 2n = 2x 20) were recovered as single additions to the haploid complement oat (Avena sativa, 6x 42) among F1 plants generated from crosses involving three different lines eight oat. In vitro rescue culture more than 4,300 immature embryos resulted in a germination frequency 11% with recovery 379 F1plantlets (8.7%) moderately vigorous growth. Some F1plants sectored distinct chromosome constitutions tillers same plant and also between root shoot...
The ancestor of barley (Hordeum vulgare subsp. spontaneum) may be a source novel alleles for crop improvement. We developed set recombinant chromosome substitution lines (RCSLs) using an accession H. spontaneum (Caesarea 26-24, from Israel) as the donor and Hordeum 'Harrington' (the North American malting quality standard) recurrent parent via two backcrosses to parent, followed by six generations selfing. Here we report (i) genomic architecture RCSLs, inferred simple sequence repeat (SSR)...
Novel plants with individual maize chromosomes added to a complete oat genome have been recovered via embryo rescue from ( Avena sativa L., 2 n = 6x 42) × Zea mays 20) crosses. An oat–maize disomic addition line possessing 21 pairs of and one chromosome 9 pair was used construct cosmid library. A multiprobe (mixture labeled fragments as probe) highly repetitive maize-specific sequences selectively isolate clones containing genomic DNA. Hybridization or their subcloned DNA revealed that most...
Single nucleotide polymorphisms (SNPs) are ideal molecular markers due to their higher abundance. Although several types of genotyping platforms for assaying large number SNPs available, in cases such as marker‐assisted selection, where few required a set potential lines, high‐throughput SNP (e.g., iScan or Infinium) may not be cost effective. In this scenario, GoldenGate assays based on VeraCode technology using Illumina BeadXpress seems the most cost‐effective platform. The objective study...
Physical map of chickpea was developed for the reference genotype (ICC 4958) using bacterial artificial chromosome (BAC) libraries targeting 71,094 clones (~12× coverage). High information content fingerprinting (HICF) these gave high-quality data 67,483 clones, and 1,174 contigs comprising 46,112 3,256 singletons were defined. In brief, 574 Mb genome size assembled in with an average 0.49 per contig represent 407 genome. The physical linked two genetic maps help 245 BAC-end sequence...
Physical mapping methods that do not rely on meiotic recombination are necessary for complex polyploid genomes such as wheat (Triticum aestivum L.). This need is due to the uneven distribution of and significant variation in genetic physical distance ratios. One method has proven valuable a number nonplant plant systems radiation hybrid (RH) mapping. work presents, first time, high-resolution map chromosome 1D (D genome) tetraploid durum (T. turgidum L., AB genomes) background. An RH panel...
Generating a contiguous, ordered reference sequence of complex genome such as hexaploid wheat (2n = 6x 42; approximately 17 GB) is challenging task due to its large, highly repetitive, and allopolyploid genome. In wheat, ordering whole-genome or hierarchical shotgun sequencing contigs primarily based on recombination comparative genomics-based approaches. However, genomics approaches are limited syntenic inference suppressed within the pericentromeric regions chromosomes, thus, precise...
Wheat, Triticum aestivum L., haploid production relies heavily on its crosses with bulbous barley, Hordeum bulbosum and anther culture, procedures influenced by genotypic specificity. Wheat × maize, Zea mays are devoid of this constraint. In study eastern gamagrass [ Tripsacum dactyloides (L.)L.) is utilized. Cross success anticipated to extend (i) the range species available for Triticeae production, (ii) crossing cycle duration. Intel‐generic T. (2 n ‐ 6 x = 42; AABBDD), turgidum L. 4 28;...
Radiation hybrid (RH) mapping is based on radiation-induced chromosome breakage and analysis of segment retention or loss using molecular markers. In durum wheat (Triticum turgidum L., AABB), an alloplasmic line [(lo) durum] has been identified with 1D T. aestivum L. (AABBDD) carrying the species cytoplasm-specific (scsae) gene. The this segregates as a whole without recombination, precluding use conventional genome mapping. A radiation population was developed from hemizygous (lo)...