A5038027615- Genetic and Environmental Crop Studies
- Agricultural pest management studies
- Legume Nitrogen Fixing Symbiosis
- Genetic and phenotypic traits in livestock
- Genetic diversity and population structure
- Plant Genetic and Mutation Studies
- Genetic Mapping and Diversity in Plants and Animals
- Plant Molecular Biology Research
- Plant Disease Resistance and Genetics
- Botanical Research and Chemistry
- Chromosomal and Genetic Variations
- Soybean genetics and cultivation
- Plant Micronutrient Interactions and Effects
- Plant tissue culture and regeneration
- Agronomic Practices and Intercropping Systems
- Genomics and Phylogenetic Studies
- Nitrogen and Sulfur Effects on Brassica
- Plant nutrient uptake and metabolism
- Cancer-related molecular mechanisms research
- Agricultural Systems and Practices
- Livestock Management and Performance Improvement
- Animal Genetics and Reproduction
- Plant Gene Expression Analysis
- Silkworms and Sericulture Research
- Plant Pathogens and Fungal Diseases
National Institute of Plant Genome Research
2016-2025
Evelina London Children's Healthcare
2025
University of Jammu
2021
Jawaharlal Nehru University
2003-2019
National Bureau of Animal Genetic Resources
1994-2011
Laboratory of Molecular Genetics
2004
Energy and Resources Institute
1993-1999
India Habitat Centre
1999
National Dairy Research Institute
1985-1992
Cicer arietinum L. (chickpea) is the third most important food legume crop. We have generated draft sequence of a desi-type chickpea genome using next-generation sequencing platforms, bacterial artificial chromosome end sequences and genetic map. The 520-Mb assembly covers 70% predicted 740-Mb length, more than 80% gene space. Genome analysis predicts presence 27,571 genes 210 Mb as repeat elements. expression performed 274 million RNA-Seq reads identified several tissue-specific...
Abstract Chickpea (Cicer arietinum) is an important food legume crop but lags in the availability of genomic resources. In this study, we have generated about 2 million high-quality sequences average length 372 bp using pyrosequencing technology. The optimization de novo assembly clearly indicated that hybrid long-read and short-read primary assemblies gave better results. a set 34,760 transcripts with 1,020 representing 4.8% (35.5 Mb) total chickpea genome. We identified more than 4,000...
Abstract Drought and salinity are the major factors that limit chickpea production worldwide. We performed whole transcriptome analyses of genotypes to investigate molecular basis drought stress response/adaptation. Phenotypic confirmed contrasting responses or stress. RNA-seq roots related was carried out under control conditions at vegetative and/or reproductive stages. Comparative analysis transcriptomes revealed divergent gene expression in different developmental identified a total 4954...
This study reports the use of Genotyping-by-Sequencing (GBS) for large-scale SNP discovery and simultaneous genotyping recombinant inbred lines (RILs) an intra-specific mapping population chickpea contrasting seed traits. A total 119,672 raw SNPs were discovered, which after stringent filtering revealed 3,977 high quality 39.5% present in genic regions. Comparative analysis using physically mapped marker loci a higher degree synteny with Medicago comparison to soybean. The data was utilized...
A transcript map has been constructed by the development and integration of genic molecular markers (GMMs) including single nucleotide polymorphism (SNP), microsatellite or simple sequence repeat (SSR) intron spanning region (ISR)-based markers, on an inter-specific mapping population chickpea, third food legume crop world first India. For SNP discovery through allele re-sequencing, primer pairs were designed for 688 genes/expressed tags (ESTs) chickpea 657 genes/ESTs closely related species...
The present study reports the large-scale discovery of genome-wide single-nucleotide polymorphisms (SNPs) in chickpea, identified mainly through next generation sequencing two genotypes, i.e.Cicer arietinum ICC4958 and its wild progenitor C. reticulatum PI489777, parents an inter-specific reference mapping population chickpea.Development validation a high-throughput SNP genotyping assay based on Illumina's GoldenGate Genotyping Technology application building high-resolution genetic linkage...
Abstract Chickpea ( Cicer arietinum L.) is an important pulse legume crop. We previously reported a draft genome assembly of the desi chickpea cultivar ICC 4958. Here we report advanced version 4958 (version 2.0) generated using additional sequence data and improved genetic map. This resulted in 2.7-fold increase length pseudomolecules substantial reduction gaps. The covered more than 94% estimated gene space predicted presence 30,257 protein-coding genes including 2230 133 encoding...
Abstract Background Chickpea ( Cicer arietinum L.) is an economically important cool season grain legume crop that valued for its nutritive seeds having high protein content. However, several biotic and abiotic stresses the low genetic variability in chickpea genome have continuously hindered molecular breeding programs. STMS (Sequence Tagged Microsatellite Sites) markers which are preferred construction of saturated linkage maps species, also emerged as most efficient reliable source...
The F-box genes constitute one of the largest gene families in plants involved degradation cellular proteins. proteins can recognize a wide array substrates and regulate many important biological processes such as embryogenesis, floral development, plant growth biotic abiotic stress, hormonal responses senescence, among others. However, little is known about legume crop, chickpea. available draft genome sequence chickpea allowed us to conduct genome-wide survey family
Summary Genomic resources such as EST s, molecular markers and linkage maps are essential for crop improvement. However, these still limited in important legumes lentil ( Lens culinaris Medik.), which is valued world wide a rich source of dietary protein. In this study, the de novo transcriptome assembly 119 855 798 short reads, generated by Illumina paired‐end sequencing, was performed using various programs. This resulted 42 196 nonredundant high‐quality transcripts average length 810...
Understanding developmental processes, especially in non-model crop plants, is extremely important order to unravel unique mechanisms regulating development. Chickpea (C. arietinum L.) seeds are valued for their high carbohydrate and protein content. Therefore, elucidate the underlying seed development chickpea, deep sequencing of transcriptomes from four stages was undertaken. In this study, next generation platform utilized sequence transcriptome distinct chickpea. About 1.3 million reads...
Seed weight and seed size both are quantitative traits have been considered as important components of grain yield, thus identification trait loci (QTL) for in lentil (Lens culinaris) would be beneficial the improvement yield. Hence main objective this study was to identify QTLs using an intraspecific mapping population derived from a cross between L. culinaris cv. Precoz (seed weight-5.1g, size-5.7mm) L830 weight-2.2g, size-4mm) comprising 126 F8-RILs. For this, two microsatellite genomic...
Physical map of chickpea was developed for the reference genotype (ICC 4958) using bacterial artificial chromosome (BAC) libraries targeting 71,094 clones (~12× coverage). High information content fingerprinting (HICF) these gave high-quality data 67,483 clones, and 1,174 contigs comprising 46,112 3,256 singletons were defined. In brief, 574 Mb genome size assembled in with an average 0.49 per contig represent 407 genome. The physical linked two genetic maps help 245 BAC-end sequence...
Summary The aim of the present study was to assess genetic variation and establish relationship amongst three Indian zebu cattle breeds using 20 bovine‐specific microsatellite markers. A total 136 unrelated DNA samples from Sahiwal (SC), Hariana (HC) Deoni (DC) were genotyped estimate within between breed diversity indices. estimated mean allelic 5.2, 6.5 5.9 in SC, HC DC, respectively, with a 167 alleles. average observed expected heterozygosity for population varied 0.42 (SC) 0.59 (DC),...
Abstract This study presents genome-wide discovery of SNPs through next generation sequencing the genome Cicer reticulatum. Mapping C. reticulatum sequenced reads onto draft assembly arietinum (desi chickpea) resulted in identification 842,104 genomic which were utilized along with an additional 36,446 genic identified from transcriptome sequences aforementioned varieties. Two new chickpea Oligo Pool All (OPAs) each having 3,072 designed and for SNP genotyping 129 Recombinant Inbred Lines...
Climate change considerably impacts plant growth and productivity by inducing stress responses. This, along with the problems of feeding ever-increasing global population, could be mitigated generating climate-resilient crop varieties enhanced productivity. However, an exhaustive account key regulatory processes that underlie developmental stress-responsive pathways is a prerequisite for improved varieties. Towards this, our study, first time, provides compilation potential impacted...