A5000108930- Cystic Fibrosis Research Advances
- Tracheal and airway disorders
- Neonatal Respiratory Health Research
- Pediatric health and respiratory diseases
- Inhalation and Respiratory Drug Delivery
- Respiratory viral infections research
- Pneumonia and Respiratory Infections
- Gut microbiota and health
- Nosocomial Infections in ICU
- Asthma and respiratory diseases
- Respiratory and Cough-Related Research
- Child Nutrition and Feeding Issues
- Nematode management and characterization studies
- Advanced biosensing and bioanalysis techniques
- Advanced Chemical Sensor Technologies
- Enterobacteriaceae and Cronobacter Research
- Antifungal resistance and susceptibility
- Antibiotics Pharmacokinetics and Efficacy
- Analytical Chemistry and Sensors
- Biosensors and Analytical Detection
- Central Venous Catheters and Hemodialysis
- Wireless Body Area Networks
- Bacterial biofilms and quorum sensing
- Microbial Community Ecology and Physiology
- Sinusitis and nasal conditions
University of Colorado Anschutz Medical Campus
2014-2025
University of Colorado Denver
2016-2025
Children's Hospital Colorado
2016-2025
Canadian Respiratory Research Network
2023
Indiana University School of Medicine
2020
The Medical Center of Aurora
2017
Culture of bronchoalveolar lavage fluid (BALF) is the gold standard for detection pathogens in lower airways cystic fibrosis (CF). However, current culture results do not explain all clinical observations CF, including negative during pulmonary exacerbation and inflammation absence pathogens. We hypothesize that organisms routinely identified by occur CF airway may contribute to disease. To test this hypothesis we used a culture-independent molecular approach, based on use rRNA sequence...
Rationale: Progressive lung function decline is a defining feature of cystic fibrosis (CF). Because airway inflammation plays central role in CF disease, inflammatory biomarkers that can be used to monitor disease activity would valuable.Objectives: Examine longitudinal relationships between sputum and function.Methods: In this prospective, cohort study, induction was performed annually over 3 years 35 children with CF. Sputum assayed for mediators related proteolysis panel...
Our objectives were to characterise the microbiota in cystic fibrosis (CF) bronchoalveolar lavage fluid (BALF), and determine its relationship inflammation disease status. BALF from paediatric adult CF patients controls undergoing clinically indicated bronchoscopy was analysed for total bacterial load by 16S rDNA sequencing. We examined 191 samples (146 45 controls) 13 centres. In aged <2 years, nontraditional taxa ( e.g . Streptococcus , Prevotella Veillonella ) constituted ∼50% of...
Rationale: Elexacaftor/tezacaftor/ivacaftor (ELX/TEZ/IVA) was shown to be efficacious and safe in patients ≥12 years of age with cystic fibrosis at least one F508del-CFTR (cystic transmembrane conductance regulator) allele, but it has not been evaluated children <12 age. Objectives: To assess the safety, pharmacokinetics, efficacy ELX/TEZ/IVA 6 through 11 F508del-minimal function or F508del-F508del genotypes. Methods: In this 24-week open-label phase 3 study, (N = 66) weighing <30 kg...
Identification of the majority organisms present in human-associated microbial communities is feasible with advent high throughput sequencing technology. As substantial variability microbiota seen across subjects, use longitudinal study designs important to better understand variation microbiome within individual subjects. Complex sample collection require analytic approaches account for this additional source variability. A common approach assessing community changes evaluate change alpha...
Background Pulmonary exacerbations (PEx), frequently associated with airway infection and inflammation, are the leading cause of morbidity in cystic fibrosis (CF). Molecular microbiologic approaches detect complex microbiota from CF samples taken during PEx. The relationship between microbiota, lung function PEx is not well understood. Objective To determine relationships subjects treated for Methods Expectorated sputum blood were collected testing performed early (0–3d.) late treatment...
Oropharyngeal (OP) swabs and induced sputum (IS) are used for airway bacteria surveillance in nonexpectorating children with cystic fibrosis (CF). Molecular analyses of these samples detect complex microbial communities. However, the optimal noninvasive sampling approach microbiota clinical relevance microbiota, particularly its relationship to inflammation, is not well characterized.The goals this study were compare molecular concurrently collected saliva, OP swabs, IS, expectorated (ES)...
Rationale: Modulation of the cystic fibrosis (CF) transmembrane conductance regulator (CFTR) protein improves clinical outcomes in patients with CF and specific CFTR genetic mutations. It remains unclear how improving function modifies existing airway infection inflammation.Objectives: To compare sputum microbiome markers inflammation before after 6 months ivacaftor treatment.Methods: The study included 31 people CF, ages 10 years older, at least one G551D allele an forced expiratory volume...
Cirrhosis (CIR) occurs in 5-7% of cystic fibrosis (CF) patients. We hypothesized that alterations intestinal function CF contribute to the development CIR. Determine frequency macroscopic lesions, inflammation, permeability and characterize fecal microbiome CIR subjects with no liver disease (CFnoLIV). 11 CFCIR (6 M, 12.8 yrs ± 3.8) 19 matched CFnoLIV (10 12.6 3.4) underwent small bowel capsule endoscopy, testing by urinary lactulose: mannitol excretion ratio, calprotectin determination...
New isolation of Pseudomonas aeruginosa (Pa) is generally treated with inhaled antipseudomonal antibiotics such as tobramycin inhalation solution (TIS). A therapeutic approach that complements traditional antimicrobial therapy by reducing the risk pulmonary exacerbation and inflammation may ultimately prolong time to Pa recurrence.
Median cystic fibrosis (CF) survival has increased dramatically over time due to several factors, including greater availability and use of antimicrobial therapies. During the progression CF lung disease, however, emergence multidrug resistance can limit treatment effectiveness, threatening patient longevity. Current planktonic-based susceptibility testing lacks ability predict clinical response chronic infections. There are numerous reasons for these limitations bacterial phenotypic...
The cystic fibrosis (CF) airway microbiome is complex; polymicrobial infections are common, and the presence of fastidious bacteria including anaerobes make culture-based diagnosis challenging. Quantitative real-time PCR (qPCR) offers a culture-independent method for bacterial quantification that may improve CF infections; however, reliability qPCR applied to specimens unknown. We sought determine nine specific assays (total bacteria, three typical pathogens, five anaerobes) specimens....
Summary Objectives To evaluate clinical outcomes associated with initial isolation of Pseudomonas aeruginosa ( Pa ) in a large U.S. cystic fibrosis (CF) cohort the current era widespread early eradication therapy. Methods Participants were children CF enrolled Early Infection Control (EPIC) Observational Study who had no from respiratory cultures prior to enrollment. Population‐averaged regression models using generalized estimating equation methods used estimate effect acquisition on...
Background Upper airway cultures guide the identification and treatment of lung pathogens in infants with cystic fibrosis (CF); however, this may not fully reflect spectrum bacteria present lower airway. Our objectives were to characterize microbiota using bronchoalveolar lavage fluid (BALF) from asymptomatic CF during first year life investigate relationship between BALF microbiota, standard culture clinical characteristics. Methods BALF, nasopharyngeal (NP) infant pulmonary function...
<h3>Objective</h3> To evaluate microbiological effectiveness, that is, culture negativity of a non-blinded eradication protocol (Rx) compared with observation (Obs) in clinically stable cystic fibrosis participants newly positive methicillin resistant <i>Staphylococcus</i><i>aureus</i> (MRSA) cultures. <h3>Design</h3> This trial randomised ages 4–45 years first or early (≤2 cultures within 3 years) MRSA-positive without MRSA-active antibiotics 4 weeks 1:1 to Rx Obs. The was: oral...
Respiratory infections caused by Pseudomonas aeruginosa and Staphylococcus aureus are the leading cause of morbidity mortality in cystic fibrosis (CF) patients. The authors aimed to identify volatile biomarkers from bronchoalveolar lavage (BAL) samples that can guide breath biomarker development for pathogen identification. BAL (n = 154) CF patients were analyzed using two-dimensional gas chromatography time-of-flight mass spectrometry. Random Forest was used select suites volatiles...