A5067646598- Bacterial biofilms and quorum sensing
- Neonatal Respiratory Health Research
- Preterm Birth and Chorioamnionitis
- Radiation Effects and Dosimetry
- SARS-CoV-2 detection and testing
- Biosensors and Analytical Detection
- Reproductive System and Pregnancy
- Respiratory viral infections research
- SARS-CoV-2 and COVID-19 Research
- Gut microbiota and health
- Bacterial Genetics and Biotechnology
- bioluminescence and chemiluminescence research
- Antimicrobial Peptides and Activities
- Listeria monocytogenes in Food Safety
- Phytase and its Applications
- Vibrio bacteria research studies
- Pregnancy and Medication Impact
- Mycobacterium research and diagnosis
- Otolaryngology and Infectious Diseases
- Image and Object Detection Techniques
- Burkholderia infections and melioidosis
- Image Retrieval and Classification Techniques
- COVID-19 diagnosis using AI
- Congenital heart defects research
- Bacillus and Francisella bacterial research
Instituto Nacional de Perinatología
2013-2025
Universidad de Los Andes
2020-2022
Universidad de Los Andes
2020
CorpoGen (Colombia)
2014-2019
Popular University of Cesar
2019
Universidad Nacional de Colombia
2019
Fundación Universitaria Sanitas
2019
Texas A&M University
2006-2010
Poultry Research Institute
2008
University of Illinois Urbana-Champaign
1999
The enteric pathogen Salmonella enterica serovar Typhimurium uses autoinducer-2 (AI-2) as a signaling molecule. AI-2 requires the luxS gene for its synthesis. regulation of global expression in by luxS/AI-2 is currently not known; therefore, focus this study was to elucidate patterns regulated luxS/AI-2. genes controlled were identified using microarrays with RNA samples from wild-type (WT) and isogenic DeltaluxS mutant, two growth conditions (presence absence glucose) at mid-log early...
Changes in respiratory tract microbiota have been associated with diseases such as tuberculosis, a global public health problem that affects millions of people each year. This pilot study was carried out using sputum, oropharynx, and nasal samples collected from patients pulmonary tuberculosis healthy control individuals, order to compare sample types their usefulness assessing changes bacterial fungal communities.Most V1-V2 16S rRNA gene sequences belonged the phyla Firmicutes,...
Massive molecular testing for COVID-19 has been pointed out as fundamental to moderate the spread of pandemic. Pooling methods can enhance efficiency, but they are viable only at low incidences disease. We propose Smart Pooling, a machine learning method that uses clinical and sociodemographic data from patients increase efficiency informed Dorfman by arranging samples into all-negative pools. To do this, we ran an automated train numerous models on retrospective dataset more than 8000...
The mother's colostrum carries immunological components, such as cytokines and immunoglobulins (Igs), derived from the maternal circulation with bacteriostatic properties.The objective of this study was to evaluate effect oropharyngeal administration (OPAC) vs. placebo in first 4 days life premature newborns ≤32 weeks gestation on serum Ig concentration, neonatal morbidity, total hospitalization.The OPAC increases Igs decreases morbidity hospitalization.A double-blind randomized controlled...
Salmonella Typhimurium is known to exhibit LuxS/AI-2–mediated cell signaling. We investigated the role of LuxS/AI-2 system on protein expression using a proteomics approach based two-dimensional gel electrophoresis (2DGE)–MALDI-MS. The global profiles wild-type, luxS mutant, and mutant strain supplemented with AI-2 were compared. Seven proteins differentially expressed when comparing wild-type strains, whereas 13 comparisons made between strains without supplementation. seven that also in...
Because of lack worldwide standardization influenza virus surveillance, comparison between countries impact a pandemic is challenging. For that, other approaches to allow internationally comparative serosurveys are welcome.Here we explore the use neonatal screening dried blood spots monitor trends 2009 A (H1N1) pdm by protein microarray.We contacted colleagues from laboratories and asked for their willingness participate in study testing anonymized bloodspots collected during course...
The highest degree of Planetary Protection (PP) applies to landed planetary missions and sample return missions. To date, Dry Heat Microbial Reduction (DHMR) treatment is the only NASA approved PP technique for meeting stringent sterility requirements life detection prevent forward contamination. However, spacecraft (s/c) payloads are made up a diverse set man-made materials components, some which incompatible with DHMR. has therefore begun investigating several complementary sterilization...
Mucormycosis caused by Apophysomyces variabilis is rarely reported in humans. A case of A. infection an immunocompetent men after friction burns a car accident described. The presented as rapidly progressive necrotizing the skin and soft tissue, which required extensive surgical debridement total colonic defunctioning colostomy associated with prolonged antifungal therapy. should be considered differential diagnosis tissue infections individuals.
We report 7 cases of melioidosis in Colombia and comparision 4 commercial systems for identifying Burkholderia pseudomallei. Phoenix were not a definitive method B. For accurate identification, we recommend including this bacterium the library databases matrix-assisted laser desorption/ionization mass spectrometry Latin America.
Summary Background COVID-19 is an acute respiratory illness caused by the novel coronavirus SARS-CoV-2. The disease has rapidly spread to most countries and territories 14·2 million confirmed infections 602,037 deaths as of July 19 th 2020. Massive molecular testing for been pointed fundamental moderate disease. Pooling methods can enhance efficiency, but they are viable only at very low incidences We propose Smart Pooling, a machine learning method that uses clinical sociodemographic data...
Microorganisms employ autoinducer molecules to modulate various bacterial processes including virulence expression, biofilm development, and bioluminescence. The universal molecule AI-2 is hypothesized mediate cell signaling in Escherichia coli O157:H7. We investigated the role of on E. O157:H7 cellular proteins using a two-dimensional (2D) gel electrophoresis-based proteomic approach. protein expression patterns between two experimental comparisons were studied namely, 1) wild type its...
Progesterone is an essential hormone that induces deep immune adaptations favoring pregnancy maintenance. We aimed at evaluating the effects of progesterone on synthesis pro- and anti-inflammatory cytokines by mononuclear cells isolated from human placental blood stimulated with lipopolysaccharide, emulating infection-inflammation environment. Mononuclear form were obtained nine women undergoing elective cesarean delivery term (not in labor), density gradient sedimentation, cultured...
The vast microbial diversity on the planet represents an invaluable source for identifying novel activities with potential industrial and therapeutic application. In this regard, metagenomics has emerged as a group of strategies that have significantly facilitated analysis DNA from multiple environments expanded limits known diversity. However, functional characterization enzymes, metabolites, products encoded by diverse genomes is limited inefficient heterologous expression foreign genes....
Abstract Problem Immune responses of fetal membranes involve the production chemoattractant mediators causing infiltration maternal and leukocytes, intrauterine inflammation potentially disruption maternal‐fetal tolerance. Prolactin (PRL) has deep immunoregulatory effects in fetal‐maternal interface. We aimed to test vitro PRL effect upon chemotactic capacities human membranes. Method Study Fetal umbilical cord blood were collected from healthy non‐laboring caesarean deliveries at term....
Lander missions and sample return (Class IVa Class IVb) require the highest degree of planetary protection (PP). To date, dry heat sterilization is only NASA approved PP technique for meeting stringent sterility requirements to prevent forward contamination. However, spacecraft payloads are made a diverse set materials components which sensitive or incompatible with such as inflatables, low-temperature adhesive, batteries, paints polymers. Electron beam (E-Beam) based ionizing irradiation...
Prolactin (PRL) is a pleiotropic hormone with key role in pregnancy. In fetal membranes, PRL can regulate the secretion of pro-inflammatory factors, which induces activation matrix metalloproteinases (MMPs). The increase and MMPs deregulate turnover extracellular altering its structure function, causing premature rupture membranes preterm labor. this work, we evaluate effect upon MMP-1, MMP-2, MMP-9, MMP-13, tissue inhibitors (TIMPs) human after lipopolysaccharide (LPS) challenge. Nine from...
Neonatal sepsis is an important public health concern worldwide due to its immediate lethality and long-term morbidity rates, Clinical evaluation laboratory analyses are indispensable for diagnosis of neonatal sepsis. However, assessing multiple biomarkers in neonates difficult limited blood availability. The aim investigate if the preterm could be identified by multiparameter analysis with flow cytometry.The expression activation-related molecules was evaluated cytometry newborn or without...