An estimated 1.5 billion microbial infections occur globally each year and result in ∼4.6 million deaths. A technology gap associated with commercially available diagnostic tests remote underdeveloped regions prevents timely pathogen identification for effective antibiotic chemotherapies infected patients. The is a trial-and-error approach that limited effectiveness, increases risk patients while contributing to antimicrobial drug resistance, reduces the lifetime of antibiotics. This paper...

This study investigates the fabrication of laser-scribed graphene (LSG) electrodes on polyimide substrates using a CO2 laser cutter for label-free L-histidine detection in artificial sweat. Two-level full factorial and central composite designs were employed to optimize critical manufacturing parameters, including speed, power, electrode width. Electrochemical characterization cyclic voltammetry with K3Fe[CN]6 demonstrated superior LSG performance compared standard glassy carbon electrodes,...

We describe and characterize a pumping mechanism that leverages the momentum present in small droplets ejected from micro-nozzle to drive flow an open microfluidic device. This approach allows driving device regime offers unique features different those achievable with typical passive or syringe-pump driven flow. Two regimes specific characteristics are described: inertia enhanced pumping, which fluid exchange times channel significantly reduced, actuated flow, it is possible initiate empty...

Surface tension driven passive pumping is a microfluidic technology that uses the surface present in small droplets to generate flow. To enhance potential of this type pumping, new 'micro pumping' technique has been developed allows for high throughput fluidic delivery by combining with droplet-based ejection system. Flow rates up four milliliters per minute (mL/min) were achieved are solely limited channel geometry and droplet size. Fluid exchange can be performed within tens milliseconds...

We demonstrate embedded growth of cortical mouse neurons in dense arrays semiconductor microtubes. The microtubes, fabricated from a strained GaAs/InGaAs heterostructure, guide axon through them and potentially enable electrical optical probing propagating action potentials. coaxial nature the microtubes—similar to myelin—is expected enhance signal transduction along axon. present technique suppressing arsenic toxicity prove success this by overgrowing neuronal cells.

Purpose The efficiency of assembly lines is a critical factor for the competitiveness industries in global market. purpose this paper to present line balancing methodology consisting combination heuristic model and an exact algorithm with intelligent task location or zone constraints. objective find minimum cost solution feasible computational time realistic function considering short‐term operation costs, task‐related workstation capital investment paralleling. Design/methodology/approach...

Gold nanoparticles (AuNPs) are the most thoroughly studied because of their remarkable optical properties. Color changes in assays that use AuNPs can be easily observed with naked eye, resulting sensitive colorimetric methods, useful for detecting a variety biological molecules. However, while represent an excellent nano-platform developing analytical methods biosensing, there still challenges must overcome before colloidal formulation successfully translated into practical applications. One...

Increasingly, invitro culture of adherent cell types utilizes three-dimensional (3D) scaffolds or aggregate strategies to mimic tissue-like, microenvironmental conditions. In parallel, new flow cytometry-based technologies are emerging accurately analyze the composition and function these microtissues (i.e., large particles) in a non-invasive high-throughput way. Lacking, however, is an accessible platform that can be used effectively sort purify particles based on analysis parameters. Here...

Water quality has a significant impact on public health. Inadequate water conditions are associated with diseases such as cholera, dysentery (shigella), hepatitis, and typhoid fever. Established techniques like Membrane Filtration (MF), Multiple Tube Fermentation (MTF), enzyme-based defined substrate technology (DST) assays used tomonitor bacteriological quality, measuring indicators Enterococcus faecalis (E. faecalis), Escherichia coli coli), total coliforms. Despite their high sensitivity...

A challenge in wearable technology lies the realtime monitoring of molecular biomarkers associated with human health. Electrochemical sensors are one most useful tools for this purpose and commonly used health devices. biosensing is particularly convenient when user-friendly, low-cost devices testing noninvasive body fluids such as sweat saliva. However, achieving high selectivity specificity measurements depends on complexity biomarker stability capture molecule. In study, laser-scribed...

Bacteriological water quality monitoring is of utmost importance for safeguarding public health against waterborne diseases. Traditional methods such as Membrane Filtration (MF), Multiple Tube Fermentation (MTF), and enzyme-based assays are effective in detecting fecal contamination indicators, but their time-consuming nature reliance on specialized equipment personnel pose significant limitations. This paper introduces a novel, portable, cost-effective UV-LED/RGB sensor that overcomes these...

Dual-sided laser ablation is used to form glass microcraters commensurate with the size of a cell. These allow for ultra-stable, low noise recordings planar patch-clamped cells.

Purpose Resource capacity and product changeovers must be both considered in the preparation of a realistic production plan. The purpose this paper is to present heuristic for enforcing resource availability accompanying changeover realities into continuous‐variable linear programming formulation that would otherwise require mixed‐integer model. results from approaches are compared terms objective function values computational requirements; effectiveness approach demonstrated....

Raman scattering (RS) was used as a powerful, efficient, and sensitive technique for studying intermolecular interactions between an organic ligand adsorbate metallic substrate. Functionalization of titanium (TiO 2 [Formula: see text]Ti) surfaces performed using lysine (Lys) later developing hydroxyapatite (HA) layer onto this functionalized surface. The functionalization process at different pH values the interacting chemical species. Chemisorption TiO Ti substrates through Lys carboxylic...

A novel microfluidic system has been developed that uses the phenomenon of passive pumping along with a user controlled droplet based fluid delivery system. Passive is by which surface tension induced pressure differences drive movement in closed channels. The automated consists set voltage valves micro-nozzles connected to reservoir and control These offer volumetrically precise way deliver droplets inlet device high frequency manner. Based on dimensions demonstrated current study example,...

Technological advances in biosensing offer extraordinary opportunities to transfer technologies from a laboratory setting clinical point-of-care applications. Recent developments the field have focused on electrochemical and optical platforms. Unfortunately, these platforms relatively poor sensitivity for most of clinically relevant targets that can be measured skin. In addition, non-specific adsorption biomolecules (biofouling) has proven limiting factor compromising longevity performance...

A novel microfluidic system has been developed that uses the phenomenon of passive pumping along with a user controlled droplet based fluid delivery system. Passive is by which surface tension induced pressure differences drive movement in closed channels. The automated consists set voltage valves micro-nozzles connected to reservoir and control These offer volumetrically precise way deliver droplets inlet device high frequency manner. Based on dimensions demonstrated current study example,...