A5101414643- MicroRNA in disease regulation
- Circular RNAs in diseases
- Bacterial Identification and Susceptibility Testing
- Antibiotic Resistance in Bacteria
- Cancer-related molecular mechanisms research
- Spectroscopy Techniques in Biomedical and Chemical Research
- Angiogenesis and VEGF in Cancer
- Advanced Biosensing Techniques and Applications
- Bacterial Infections and Vaccines
- Infective Endocarditis Diagnosis and Management
- Cancer, Hypoxia, and Metabolism
- Biosensors and Analytical Detection
- Cancer, Lipids, and Metabolism
- Urinary Tract Infections Management
- Molecular Biology Techniques and Applications
- Estrogen and related hormone effects
- Protein Degradation and Inhibitors
- TGF-β signaling in diseases
- Pharmacy and Medical Practices
- Cancer Mechanisms and Therapy
- Cancer Genomics and Diagnostics
- Paraoxonase enzyme and polymorphisms
- Lymphatic System and Diseases
- interferon and immune responses
- Biochemical Acid Research Studies
Capital Medical University
2008-2022
Beijing Tian Tan Hospital
2010-2022
Beihang University
2020-2022
National Clinical Research Center for Digestive Diseases
2020
Harbin Medical University
2006
Purpose To explore the expression pattern, prognostic value and functional role of miR-196b in glioblastoma (GBM) patients using large cohorts. Experimental Design MiR-196b was measured Human v2.0 miRNA Expression BeadChip (Illumina) 198 frozen glioma tissues. The levels were also validated an independent cohort containing 128 formalin-fixed paraffin-embedded (FFPE) samples qRT-PCR. presence other molecular indicators assessed centrally samples. Whole genome gene profiling performed to...
Ubiquitin-conjugating enzyme E2C (UBE2C) is a key regulator of cell cycle progression and involved in the tumorigenesis variety cancers. Previous studies have demonstrated that UBE2C an important factor malignant astrocytic tumors. However, association between expression clinical prognosis glioma patients has not been defined. In present study, gliomas non-cancerous brain tissues were detected by microarray immunohistochemical analysis. The clinicopathological characteristics was evaluated....
Post-neurosurgical meningitis (PNM) is one of the most severe hospital-acquired infections worldwide, and a large number pathogens, especially those possessing multi-resistance genes, are related to these infections. Existing methods for detecting bacteria measuring their response antibiotics lack sensitivity stability, laboratory-based detection inconvenient, requiring at least 24h complete. Rapid identification determination susceptibility urgently needed, in order combat emergence...
Abstract Glioma is the most prevalent malignant cancer in central nervous system and can cause significant mortality morbidity. A rapid, convenient, accurate, relatively noninvasive diagnostic method for glioma important urgently needed. In this study, we investigated feasibility of using Raman spectroscopy to discriminate patients with from healthy individuals. Serum samples were collected individuals ( n = 86) [high‐grade (HGG) 75, low‐grade (LGG) 60]. All spectra a 785‐nm wavelength laser...
Low-grade glioma is predisposed to progress anaplastic astrocytoma and eventually secondary glioblastoma. The malignant transformation may involve the accumulation of multiple genetic alterations. purpose this study was explore role miR-544 in progression discuss whether it be a novel biomarker transformation. expression measured series 198 samples (63 low-grade glioma, 44 91 glioblastoma tumors) using microarrays. Quantitative real-time reverse transcription PCR (qRT-PCR) used validate...
Cell division cycle 6 (cdc6) is a key factor of DNA replication initiation license system and proto-oncogene. It has been detected in some tumor tissues to aid cancer diagnosis many research projects. However, it remains unclear that if cdc6 could be the peripheral blood, just like liquid biopsy, solid patients. The aim this study investigate possibility as biomarker for circulating cells patients with lung cancer.We first expression blood mononuclear (PBMCs) by situ hybridization RNA probe....
Abstract Central nervous system infection (CNSI) is a significant type of that plagues the fields neurology and neurosurgical science. Prompt accurate diagnosis CNSI major challenge in clinical laboratory assessments; however, developing new methods may help improve diagnostic protocols. This study evaluated second-generation micro/nanofluidic chip platform (MNCP-II), which overcomes difficulties diagnosing bacterial fungal infections CNS. The MNCP-II simple to operate, can identify 44 genus...
Rapid antimicrobial susceptibility testing (AST) is urgently needed to slow down the emergence of antibiotic-resistant bacteria and treat infections with correct antibiotics. Stimulated Raman scattering (SRS) microscopy a technique that enables rapid chemical-bond imaging sub-cellular resolution. It can obtain AST results single bacterium Although SRS assay relatively fast, taking less than 2 h, calculation single-cell metabolism inactivation concentration (SC-MIC) performed manually takes...
Abstract Background Bone marrow-derived endothelial stem cells participate in vascular repairs. Numbers of circulating progenitor (cEPCs) are associated with atherosclerosis. Fibrinogen plays a key role Objective was to assess if cEPC counts were atherosclerotic intracranial artery stenosis (IAS). Methods Three hundred subjects (108 patients stroke and IAS (IAS), 120 control without (CP), 72 healthy controls (HC)) retrospectively analyzed. cEPCs identified counted by flow cytometry using...
Emerging evidence suggests that DEAH-box polypeptide 32 (DHX32) serves an important role in the progression and metastasis of cancer. However, DHX32 breast cancer remains to be completely elucidated. The aim present study was evaluate expression clinical significance reverse transcription-quantitative polymerase chain reaction performed analyze messenger (m)RNA expression, western blotting immunohistochemistry were examine protein adjacent non-cancerous tissues. association between...
There is an urgent need to develop a rapid procedure that can rapidly identify and obtain antimicrobial susceptibility testing (AST) results directly from positive blood cultures. Here, we report semi-automatic bacterial diagnosis procedure, which includes (1) concentration process isolate bacteria culture bottle (PBCB), (2) identification using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS), (3) AST based on stimulated Raman scattering imaging of...
The susceptibilities of vertilmicin and seven reference aminoglycosides to modifications by six recombinant aminoglycoside-modifying enzymes, AAC(6')-Ie, APH(2'')-Ia, AAC(6')-Ie-APH(2'')-Ia, ANT(2'')-Ia, AAC(6')-Ib, AAC(6')-Ib-cr, were studied coupled spectrophotometric assays in microtiter plates. In comparison other aminoglycosides, the susceptibility was 45.8- 250.0-fold lower for AAC(6')-Ie acetylation, 39.2- 116.7-fold AAC(6')-Ie-APH(2'')-Ia 1.8- 7.5-fold ANT(2'')-Ia adenylation (except...
The existing identification (ID) and antimicrobial susceptibility testing (AST) method requires at least two to three days detect blood infection, a fast accurate detection is very necessary for sepsis patients or Intensive Care Unit (ICU) patients. Here, we describe direct isolated bacteria from positive culture bottle (PBCB), rapid AST by femtosecond stimulated Raman scattering (SRS) imaging of deuterium oxide (D2O) metabolism, which can determine the PBCB in 5-6 hours. sample passed...