Post-neurosurgical meningitis (PNM) is one of the most severe hospital-acquired infections worldwide, and a large number pathogens, especially those possessing multi-resistance genes, are related to these infections. Existing methods for detecting bacteria measuring their response antibiotics lack sensitivity stability, laboratory-based detection inconvenient, requiring at least 24h complete. Rapid identification determination susceptibility urgently needed, in order combat emergence...

Our study is a retrospective observational conducted in one of the largest clinical centers neurosurgery China. We aimed to investigate antimicrobial susceptibility patterns Enterobacteriaceae isolates responsible for nosocomial meningitis/encephalitis post-neurosurgical patients. Meanwhile, we tried evaluate risk factors mortality following meningitis/encephalitis.Medical data on characteristics, antibiotic susceptibilities, and were reviewed until patients' discharge or death hospital....

Objectives This is a comparative cohort study aiming to evaluate the mortality risk factors for patients with nosocomial meningitis (NM) induced by multidrug-resistant Enterobacteriaceae (MDRE) in China. The clinical features and therapies of resistance mechanisms MDRE pathogens were also assessed. Methods MDRE-NM from two neurosurgical centers China 2014 2019 included this study. Clinical extracted medical record databases centers. molecular underlying microbiological each pathogen...

Purpose The problem of port and starboard ambiguity will exist when only utilize the vector or scalar parameters. Meanwhile, amplitude-phase error between can also cause this problem. In paper, a compound MEMS hydrophone which contains cilia microstructure piezoelectric ceramic tube has been presented to solve Compared with traditional hydrophone, realize measurement sound pressure vibration velocity simultaneously. Design/methodology/approach A presented. unipolar directivity combined...

Metagenomic next-generation sequencing (mNGS) has become a widely used technology that can accurately detect individual pathogens. This prospective study was performed between February 2019 and September in one of the largest clinical neurosurgery centers China. The aimed to evaluate performance mNGS on cerebrospinal fluid (CSF) from neurosurgical patients for diagnosis external ventricular lumbar drainage (EVD/LD)-associated ventriculitis meningitis (VM). We collected CSF specimens with...

In this work, we demonstrated a silicon nanowire (SiNW) biosensing platform capable of simultaneously identifying different Dengue serotypes on single sensing chip. Four peptide nucleic acids (PNAs), specific to each (DENV-1 DENV-4), were spotted areas the SiNW array surface, and covalently immobilized PNA probes then interacted with target establish specificity detection. Detection scheme is based changes in resistances due accumulation negative charges contributed by hybridized DNA target....

The bionic cilium MEMS vector hydrophone has the characteristics of low power consumption, small volume, and good low-frequency response. Nevertheless, there exists problem left–right ambiguity in azimuth estimation a single hydrophone. In order to solve engineering application problem, sound-pressure sound-pressure-gradient is designed this paper. new composite consists two channels. microstructure optimized used as channel, collect sound pressure gradient information, scalar based on...

Background The differential diagnosis between postneurosurgical bacterial meningitis and aseptic remains challenging both for the clinician laboratory. Combinations of markers, as opposed to single ones, may improve thereby survival. Methods This prospective cohort study included patients with suspected after neurosurgery. were divided into two groups according diagnostic criteria involving a group group. Four biomarkers, including cerebrospinal fluid procalcitonin, lactate, interleukin-8...

EphB4 belongs to the largest family of Eph receptor tyrosine kinases. It contributes a variety pathological progresses cancer malignancy. However, little is known about its role in neural stem cells (NSCs). This study examined whether required for proliferation and differentiation human embryonic (hNSCs) vitro.We up- down-regulated expression hNSCs using lentiviral over-expression shRNA knockdown constructs then investigated influence on properties hNSCs.Our results show that shRNA-mediated...

Abstract Objective To evaluate the diagnostic accuracy of cerebrospinal fluid (CSF)–based routine clinical examinations for post‐neurosurgical bacterial meningitis (PNBM) in multicenter patients. Methods The accuracies to distinguish between PNBM and aseptic (PNAM) were evaluated by determining values area under curve (AUC) receiver operating characteristic a retrospective analysis patients four centers. Results An algorithm was constructed using logistic as classical method maximize...

Three-dimensional vector hydrophone plays an important role in underwater spatial location. In order to solve the problems of monolithic integration and linearity previous three-dimensional hydrophones, a micro-electro-mechanical system (MEMS) based on piezoresistive effect bio-inspired principle is proposed this paper. Different from hydrophone, MEMS monolithically integrated. It has characteristics high consistency batch production. Acoustic pressure gradients xand y-directions are...

Abstract Central nervous system infection (CNSI) is a significant type of that plagues the fields neurology and neurosurgical science. Prompt accurate diagnosis CNSI major challenge in clinical laboratory assessments; however, developing new methods may help improve diagnostic protocols. This study evaluated second-generation micro/nanofluidic chip platform (MNCP-II), which overcomes difficulties diagnosing bacterial fungal infections CNS. The MNCP-II simple to operate, can identify 44 genus...

Abstract Background To determine the phenotype, molecular characterisation and risk factors of postoperative meningitis induced by Extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae (EPE) in China. Methods We performed a multi-centre comparative cohort study patients infected with 4 neurosurgical centres China from January 2014 to December 2019. Phenotype characteristics isolates were reviewed tested, independent EPE evaluated binary logistic regression. Results In total, 220...

To explore the clinical characteristics of post-neurosurgical meningitis (PNM) patients infected with nonfermenting Gram-negative bacilli (NFGNB) and to evaluate related mortality risk factors.A cohort analysis PNM NFGNB in Beijing Tiantan Hospital Capital Medical University from 2012.1 2020.12. The microbial distribution, antimicrobial sensitivity genotypes were tested, potential factors evaluated using Mann-Whitney U or chi-squared tests. Independent for established by constructing a...

A new type of array MEMS (Microelectro Mechanical Systems) vector hydrophone has been proposed to solve the left-right ambiguity problem that is commonly found in current ones. Meanwhile, advantages good sensitivity and low fabrication cost are maintained. The integrated by four units oriented at different direction angles. By aid this kind hydrophone, not only exact sound source can be measured, but also position obtained. working principle microstructure analyzed simulated. result shows...

Ultrasound computed tomography (USCT) systems based on capacitive micromachined ultrasonic transducer (CMUT) arrays have a wide range of application prospects. For this paper, high-precision image reconstruction method the propagation path ultrasound in breast tissue are designed for CMUT ring array; that is, time-reversal algorithms and FBP respectively used to reconstruct sound speed distribution acoustic attenuation distribution. The feasibility is verified by numerical simulation model...

The potential of obtaining incremental diagnostic information using a parallel assay is attractive.