A5100418902- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- Advanced Biosensing Techniques and Applications
- RNA Interference and Gene Delivery
- Quantum Dots Synthesis And Properties
- SARS-CoV-2 detection and testing
- Electrochemical sensors and biosensors
- Electrochemical Analysis and Applications
- Innovative Microfluidic and Catalytic Techniques Innovation
- Nanopore and Nanochannel Transport Studies
- CRISPR and Genetic Engineering
- Viral gastroenteritis research and epidemiology
- Acoustic Wave Phenomena Research
- Nonlinear Optical Materials Studies
- Advanced Nanomaterials in Catalysis
- Microfluidic and Capillary Electrophoresis Applications
- Viral Infections and Outbreaks Research
- Microfluidic and Bio-sensing Technologies
- Supramolecular Self-Assembly in Materials
- Plasmonic and Surface Plasmon Research
- Gold and Silver Nanoparticles Synthesis and Applications
- Luminescence and Fluorescent Materials
- SARS-CoV-2 and COVID-19 Research
- Cancer-related gene regulation
- Insect symbiosis and bacterial influences
Southwest University
2018-2025
State Grid Corporation of China (China)
2024
University of California, Riverside
2017-2024
Southwest Forestry University
2024
Affiliated Hospital of Southwest Medical University
2024
China Academy of Launch Vehicle Technology
2024
Guangzhou Institute of Energy Conversion
2023
South China Agricultural University
2022-2023
Rochester Institute of Technology
2020-2022
Beijing University of Technology
2022
Exosomal microRNAs are essential in intercellular communications and disease progression, yet it remains challenging to quantify the expression level due their small size low abundance blood. Here, we report a "sandwich" electrochemical exosomal microRNA sensor (SEEmiR) detect target with high sensitivity specificity. In SEEmiR, neutrally charged peptide nucleic acid (PNA) enables kinetically favorable hybridization relative negatively DNA, particularly short sequence (10 nt). More...
DNA self-assembly has created various nanostructured probes deployed in biosensors, whereas their direct charge contribution to sensitive bioassays remains elusive. Here, we report a supercharged tetrahedral nanolabel-based electrochemical (eTDN) sensor for ultrasensitive detection of exosomal microRNAs (Exo-miRs). By using an "assembly before testing" strategy, there is high-efficiency recognition between the target Exo-miR and self-assembled TDN probe homogenous solution relative...
Salmonella species are common foodborne pathogenic bacteria. At present, most detection methods for unsuitable on-site applications because they require large instruments or complicated procedures. This study developed a novel method of the in food by combining CRISPR/Cas12a system with recombinant polymerase amplification (RPA). The optimal concentration ratio Cas12a enzyme, CRISPR RNA (crRNA) and FQ-probe is 1 : 1: 2.5. limit RPA–CRISPR/Cas12a was × 10−4 ng/μL genomic DNA (gDNA), 102...
Abstract A novel and ultrasensitive sandwich-type electrochemical immunosensor was designed for the quantitative detection of carcino-embryonic antigen (CEA). This developed by using trimetallic NiAuPt nanoparticles on graphene nanosheets (NGs) (NiAuPt-NGs) as excellent labels β-cyclodextrin functionalized reduced oxide (CD-NGs) platform. The CD-NGs with high specific surface area good biocompatibility ideal dispersibility used to capture primary antibodies (Ab 1 ) efficiently. NiAuPt-NGs...
A novel localized surface plasmon resonance (LSPR) system based on the coupling of gold nanomushrooms (AuNMs) and nanoparticles (AuNPs) is developed to enable a significant plasmonic resonant shift. The AuNP size, chemistry, concentration are characterized maximize LSPR effect. 31 nm redshift achieved when AuNMs saturated by AuNPs. This giant also increases full width spectrum explained 3D finite-difference time-domain (FDTD) calculation. In addition, this substrate packaged in microfluidic...
Computer vision has become a powerful tool in the field of biosensing, aiding development innovative and precise systems for analysis interpretation biological data. This interdisciplinary approach harnesses capabilities computer algorithms techniques to extract valuable information from various biosensing applications, including medical diagnostics, environmental monitoring, food health. Despite years development, there is still significant room improvement this area. In perspective, we...
Non-restorative sleep is prevalent among individuals with depression and strongly associated the severity of condition. Therefore, identifying non-restorative can aid in early screening depression. Investigating necessitates long-term monitoring under naturalistic conditions. In this study, we recruited 149 participants collected electrocardiogram triaxial acceleration from them, resulting a total 761 nights data. The period midnight to 6:30 AM was segmented into 78 five-minute intervals,...
A fully Integrated Micropillar Polydimethylsiloxane Accurate CRISPR deTection (IMPACT) system is developed for viral DNA detection. This powerful patterned with high-aspect-ratio micropillars to enhance reporter probe binding. After surface modification and immobilization, the CRISPR-Cas12a/crRNA complex injected into enclosed microchannel. With presence of a double-stranded target, enzyme activated denatures single-stranded reporters from micropillars. collateral cleavage releases...
Beginning from the end of 2019, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic swept all over world and is still afflicting whole global population. Given that vaccine-manufacturing ability limited virus can evolve quickly, vaccination alone may not be able to pandemic, thus developing fast accurate diagnoses effective therapeutics will always unmet needs. Phage display peptide library has been used in screening antigen-specific peptides for invention novel mimic...
A gold nanoparticle (AuNP) labeled CRISPR-Cas13a nucleic acid assay has been developed for sensitive solid-state nanopore sensing. Instead of directly detecting the translocation RNA through a nanopore, our system utilizes non-covalent conjugates AuNPs and targets. Upon CRISPR activation, are liberated from RNA, isolated, passed sensor. Detection can be observed as increasing ionic current in chip. Each AuNP that is detected enumerated an event, leading to quantitative molecular Leveraging...
Pathogenic variants of Burkholderia gladioli pose a serious threat to human health and food safety, but there is lack rapid sensitive field detection methods for gladioli. In this study, the CRISPR/Cas12a system combined with recombinant enzyme polymerase amplification (RPA) was used detect in food. The optimized RPA-CRISPR/Cas12a assay able specifically stably at constant 37 °C without assistance large equipment. limit method evaluated two aspects, genomic DNA (gDNA) level bacterial...
With the widespread contamination of ochratoxin A (OTA), it is significant importance for detecting OTA in foods and traditional Chinese medicine (TCM). In this study, a novel label-free fluorescent aptasensor utilizing interaction between OTA-triggered antiparallel G-quadruplex (N-methyl-4-pyridy) porphyrin (TMPyP) rapid sensitive determination was established. The fluorescence CdTe quantum dots (QDs) could be quenched by TMPyP. presence analyte aptamer recognize transform from random coil...
Myocardial ischemia is featured by a significant increase in the cytoplasm proton concentration, and such change may be applied as an index for earlier ischemic heart disease diagnostics. But pH live cell difficult to monitor normal fluorescent probe cannot specifically transport into of cell. This because contains condensed myofibrils which are tight barriers penetrate. We design probes, single-strand DNA wrapped single-wall carbon nanotubes (ssDNA-SWCNTs), where ssDNA labeled dye molecule...
An individual nanoparticle-based plasmonic nanotechnology was used for real-time monitoring of lectin–sugar interactions, which could be designed as novel nanobiosensors the detection trace concanavalin A (ConA) with high sensitivity and selectivity. The localized surface plasmon resonance (LSPR) spectra Au@Ag nanocubes (NCs) are linearly shifted to a long wavelength an increasing concentration ConA. In fact, each NC can act nanobiosensor quantified ConA, enables miniaturization biosensor...