A5069922239- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- CRISPR and Genetic Engineering
- SARS-CoV-2 detection and testing
- RNA modifications and cancer
- Innovative Microfluidic and Catalytic Techniques Innovation
- interferon and immune responses
- Cardiovascular Effects of Exercise
- Endoplasmic Reticulum Stress and Disease
- Cancer-related cognitive impairment studies
- Simulation and Modeling Applications
- Microfluidic and Bio-sensing Technologies
- Advanced Electron Microscopy Techniques and Applications
- Nanowire Synthesis and Applications
- Cardiomyopathy and Myosin Studies
- Histiocytic Disorders and Treatments
- Aluminum Alloys Composites Properties
- Catalytic Processes in Materials Science
- RNA regulation and disease
- Structural Health Monitoring Techniques
- Virus-based gene therapy research
- Industrial Technology and Control Systems
- Poxvirus research and outbreaks
- Silicon Nanostructures and Photoluminescence
- Electron and X-Ray Spectroscopy Techniques
University of California, Riverside
2022-2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2025
Academy of Medical Sciences
2025
Qingdao University
2022-2024
Affiliated Hospital of Qingdao University
2024
Rochester Institute of Technology
2020-2021
Hefei University of Technology
2019
National University of Defense Technology
2019
Wan Fang Hospital
2001
The global outbreak of the monkeypox virus (MPXV) highlights need for rapid and cost-effective MPXV detection tools to effectively monitor control disease. Herein, we demonstrated a portable CRISPR-Cas-based system naked-eye MPXV. harnesses high selectivity CRISPR-Cas12 isothermal nucleic acid amplification potential recombinase polymerase amplification. It can detect both current circulating clade original clades. We reached limit (LoD) 22.4 aM (13.5 copies/µl) using microtiter plate...
A multiplexing nano-sieve device is developed to couple with CRISPR assay for the sensitive, accurate, and specific detection of drug-resistant bacteria in biofluids.
<title>Abstract</title> <italic>Nothapodytes nimmoniana</italic> is known to produce the highest content of anticancer compound camptothecin (CPT) in plant kingdom. We present chromosome-level allotetraploid genome <italic>N. nimmoniana</italic>, marking first sequence from order Icacinales. This 5-Gb encodes 92,630 genes, with subgenome B exhibiting dominant gene expression. Through mining, we identified and characterized four key enzymes involved CPT biosynthesis, revealing that shares a...
Abstract This study demonstrates a biosensing platform facilitated by localized surface plasmonic resonance (LSPR) on silicon (Si) nanopillar metasurface mediated the presence of cephalexin (Cef) antibiotics in solution. The is designed to exhibit narrow quadrupolar Mie resonances that when coupled with bovine serum albumin‐coated (BSA‐coated) gold nanospheres (BSANS) will produce an appreciable redshift at peak wavelength, occurring only target antibiotic. To optimize performance Si...
Beginning from the end of 2019, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic swept all over world and is still afflicting whole global population. Given that vaccine-manufacturing ability limited virus can evolve quickly, vaccination alone may not be able to pandemic, thus developing fast accurate diagnoses effective therapeutics will always unmet needs. Phage display peptide library has been used in screening antigen-specific peptides for invention novel mimic...
A fast, easy-to-implement, highly sensitive, and point-of-care (POC) detection system for frog virus 3 (FV3) is proposed. Combining recombinase polymerase amplification (RPA) CRISPR/Cas12a, a limit of (LoD) 100 aM (60.2 copies/μL) achieved by optimizing RPA primers CRISPR RNAs (crRNAs). For POC detection, smartphone microscopy implemented, an LoD 10 in 40 min. The proposed detects four positive animal-derived samples with quantitation cycle (Cq) value quantitative PCR (qPCR) the range 13 to...
A simple, portable, and low-cost microfluidic system-funnel adapted sensing tube (FAST) is developed as an integrated, power-free, pipette-free biosensor for viral nucleic acids. This FAST chip consists of four reaction chambers separated by carbon fiber rods, the reagents in each chamber are transferred mixed manually removing rods. Rather than using electrical heaters, only a hand warmer pouch used isothermal recombinase polymerase amplification (RPA) CRISPR-Cas12a reaction. The signal...
CRISPR technology has gained widespread adoption for pathogen detection due to its exceptional sensitivity and specificity. Although recent studies have investigated the potential of high-aspect-ratio microstructures in enhancing biochemical applications, their application CRISPR-based been relatively rare. In this study, we developed a FRET-based biosensor combination with Cas12a-mediated trans-cleavage detecting HPV 16 DNA fragments. Remarkably, our results show that micropillars higher...
Using porous silicon (PSi) interferometer sensors, we show the first experimental implementation of high contrast cleavage detection (HCCD) mechanism. HCCD makes use dramatic optical signal amplification caused by high-contrast nanoparticle labeled reporters instead capture low-index biological molecules. An approximately 2 nm reflectance peak shift was detected after DNA-quantum dot from PSi surface via exposure to a 12.5 nM DNase enzyme solution. This change is 20 times greater than...
ABSTRACT A fully Integrated Micropillar Polydimethylsiloxane Accurate CRISPR Detection (IMPACT) system is developed for viral DNA detection. This powerful patterned with high-aspect ratio micropillars to enhance reporter probe binding. After surface modification and immobilization, Cas12a/crRNA complex injected into the enclosed system. With presence of double-stranded target, enzyme activated non-specifically cleaves ssDNA reporters initially immobilized on micropillars. collateral cleavage...
摘要: 在分析平衡重式叉车的结构特性以及侧翻机理的基础上,将叉车的侧倾状况分为一级侧倾状况和二级侧倾状况,并提出一种平衡重式叉车的横向稳定性侧倾分级控制策略,叉车侧倾控制的执行机构设计为一种新型三连杆液压支撑调整机构;平衡重式叉车的侧倾分级控制器的设计采取变论域模糊控制方法,当叉车处于一级侧倾状况时,基于侧倾变论域的模糊控制的级别为一级;当叉车处于二级侧倾状况时,基于侧倾变论域的模糊控制的级别为二级;基于ADAMS建立叉车整车虚拟样机模型并与Matlab/Simulink进行联合仿真和实车试验验证,结果表明采取基于侧倾分级的叉车横向稳定性变论域的模糊控制策略可有效提高叉车的横向稳定性。
Fowl adenovirus serotype 4 (FAdV-4) is recognized as a pathogen that causes hydropericardium syndrome. Irrespective of the pathway used by virus to invade chicken, pathological characteristics disease include degeneration and necrosis hepatocytes, formation intranuclear inclusions, well inflammatory cell infiltration. Liver dysfunction constitutes one critical factors leading death. Therefore, it vital investigate virus-mediated severe liver damage further understand pathogenesis FAdV-4....
Abstract Quantitative analysis of human papillomavirus (HPV)-infected cervical cancer is essential for early diagnosis and timely treatment cancer. Here, we introduce a novel energy transfer-labeled oligonucleotide probe to enhance the loop-mediated isothermal amplification (LAMP) assay highly sensitive specific detection HPV 16. Conducted as single-step within digital nanofluidic chip featuring numerous reaction reservoirs, our method facilitates target under conditions. Targeting an 16...
We present a novel energy transfer-labeled oligonucleotide probe designed to enhance the sensitivity and specificity of LAMP assay for HPV 16 detection. Our approach achieves detection limit 1 fM spans dynamic range five orders magnitude.
Microfluidic-based sheath flow focusing methods have been widely used for efficiently isolating, concentrating, and detecting pathogenic bacteria various biomedical applications due to their enhanced sensitivity exceptional integration. However, such a microfluidic device usually needs complicated fabrication sample dilution, hampering the efficient sensitive identification of target bacteria. In this study, we develop fabricate sheath-assisted pneumatic-induced nano-sieve achieving improved...
The intelligent water-saving valve without power supply is an equipment characterized by high automation, low costs and controlling supply, which targeted to solve problems of irrigation all around the world. To make this apply in production better, paper does research on transmission rate moisture between humidity sensitive materials external environment, illustrates three rules: (1) For different types soil, fastest soil could be found sand slowest one clay. (2) ratios materials, material...
A portable, inexpensive, and easy-to-manufacture microfluidic device is developed for the detection of SARS-CoV-2 dsDNA fragments. In this device, four reaction chambers separated by carbon fiber rods are pre-loaded with isothermal amplification CRISPR-Cas12a reagents. The carried out simply pulling rods, without need manual pipetting. To facilitate power-free pathogen detection, entire designed to be heated a disposable hand warmer. After CRISPR reaction, fluorescence signal generated...