A5045136847- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Atherosclerosis and Cardiovascular Diseases
- Nitric Oxide and Endothelin Effects
- Cell Adhesion Molecules Research
- Adipokines, Inflammation, and Metabolic Diseases
- Cytomegalovirus and herpesvirus research
- Acute Ischemic Stroke Management
- Neuroinflammation and Neurodegeneration Mechanisms
- Blood Coagulation and Thrombosis Mechanisms
- Platelet Disorders and Treatments
- Antiplatelet Therapy and Cardiovascular Diseases
- Cardiac Ischemia and Reperfusion
- Immune Response and Inflammation
- Sepsis Diagnosis and Treatment
- Mesenchymal stem cell research
- Eicosanoids and Hypertension Pharmacology
- Neurological Disease Mechanisms and Treatments
- Venous Thromboembolism Diagnosis and Management
- Advanced Glycation End Products research
- Sulfur Compounds in Biology
- Inflammatory Bowel Disease
- Extracellular vesicles in disease
- Heat shock proteins research
- Immune Cell Function and Interaction
- Thermal Regulation in Medicine
Louisiana State University Health Sciences Center Shreveport
2015-2025
Louisiana State University
2003-2023
Louisiana State University Health Sciences Center New Orleans
2007-2023
Cellular Research (United States)
2019
National Institute of Allergy and Infectious Diseases
2018
National Institutes of Health
2018
Karen Hospital
2013-2015
City University of New York
2011
University Hospital, Newark
2011
National Center for Tumor Diseases
2011
Although lymphocyte recruitment and activation are associated with cerebral ischemia-reperfusion (I/R) injury, the contributions of specific subpopulations lymphocyte-derived interferon-gamma (IFN-gamma) to stroke remain unknown. The objectives this study were define contribution populations lymphocytes inflammatory prothrombogenic responses elicited in microvasculature by I/R investigate role T-cell-associated IFN-gamma pathogenesis ischemic stroke.Middle artery occlusion was induced for 1...
Abstract Inflammatory cell recruitment to local sites of tissue injury and/or infection is controlled by a plethora signalling processes influencing cell-to-cell interactions between the vascular endothelial cells (ECs) in post-capillary venules and circulating leukocytes. Recently, ATP-sensitive P 2 Y purinergic receptors have emerged as downstream regulators EC activation inflammation. However, mechanism(s) regulating cellular ATP release this response remains elusive. Here we report that...
The nitrite anion is an endogenous product of mammalian nitric oxide (NO) metabolism, a key intermediate in the nitrogen cycle plants, and constituent many foods. Research over past 6 years has revealed surprising biological cytoprotective activity this anion. Hypercholesterolemia causes proinflammatory phenotype microcirculation. This appears to result from decline NO bioavailability that results reduction biosynthesis, inactivation by superoxide, or both. Since been shown be potently...
Although chemokines have been implicated in cardiovascular diseases, few studies addressed the role of these inflammatory mediators ischemic stroke. This study tested hypothesis that RANTES (CCL5; regulated on activation, normal T-cell expressed and secreted) mediates cerebral microvascular dysfunction, inflammation, tissue injury induced by brain ischemia reperfusion.After 60-minute middle artery occlusion reperfusion, adhesion leukocytes platelets venules, infarct volume, blood-brain...
Ischemia reperfusion injury (I/RI) is a common complication of cardiovascular diseases. Resolution detrimental I/RI-generated prothrombotic and proinflammatory responses essential to restore homeostasis. Platelets play crucial part in the integration thrombosis inflammation. Their role as participants resolution thromboinflammation underappreciated; therefore we used pharmacological genetic approaches, coupled with murine clinical samples, uncover key concepts underlying this role.Middle...
Abstract While heart disease remains a common cause of mortality, cerebrovascular also increases with age, and has been implicated in Alzheimer's related dementias (ADRD). We have described hydrogen sulfide (H 2 S), signaling molecule important vascular homeostasis, as biomarker cardiovascular disease. hypothesize that plasma H S its metabolites relate to cognitive dysfunction ADRD. used analytical biochemical methods measure MRI evaluate indicators microvascular Levels total specific were...
CD40/CD40 ligand (CD40L) signaling contributes to proinflammatory and prothrombogenic responses in the vasculature. CD40/CD40L expression is elevated patients after a transient ischemic attack or stroke. The purpose of this study was investigate role cerebral microvascular dysfunction tissue injury response middle artery occlusion (MCAO) reperfusion.Intravital fluorescence microscopy used visualize microcirculation wild-type (WT), CD40-deficient, CD40L-deficient mice subjected 1-hour MCAO...
Although epidemiological studies reveal an increased incidence of obesity and association between the prevalence/severity ischemic stroke, little is known about mechanisms that link to stroke. This study tested hypothesis exacerbates cerebrovascular dysfunction tissue injury induced by brain ischemia reperfusion.The adhesion leukocytes platelets in cerebral venules, blood-brain barrier permeability, water content, infarct volume were measured wild-type, obese (ob/ob), leptin-reconstituted...
We previously demonstrated that diabetes exacerbates stroke-induced brain injury, and this correlates with methylglyoxal (MG)-to-glutathione (GSH) status. Cerebral injury was reversed by N-acetylcysteine (NAC). Here we tested if the pro-thrombotic phenotype seen in systemic circulation during associated increased MG-glycation of proteins, NAC could reverse this. The streptozotocin (STZ)-induced mouse model type 1 used. Thrombus formation venules arterioles (pial circulation) determined...
Abstract Methamphetamine-associated cardiomyopathy is the leading cause of death linked with illicit drug use. Here we show that Sigmar1 a therapeutic target for methamphetamine-associated and defined molecular mechanisms using autopsy samples human hearts, mouse model “binge crash” methamphetamine administration. expression significantly decreased in hearts users those methamphetamine-treated mice. The also signs cardiomyopathy, including cellular injury, fibrosis, enlargement heart. In...
Abstract —Experimental animals placed on a high-cholesterol diet for 2 or more weeks exhibit an inflammatory response in postcapillary venules. The aims of this study were to determine (1) whether superoxide mediates the hypercholesterolemia-induced and (2) leukocyte and/or vessel wall NAD(P)H oxidase contributes response. Intravital videomicroscopy was used quantify leukocyte–endothelial cell adhesion cremasteric venules wild-type (WT) mice, CuZn-superoxide dismutase transgenic (SOD TgN)...
Although hypercholesterolemia is widely accepted as a major risk factor for coronary artery and peripheral vascular diseases, its role in the pathogenesis of stroke controversial. The objectives this study were to determine how affects cerebral microcirculation under resting conditions after ischemia-reperfusion (I/R). Platelet- leukocyte-endothelial cell interactions oxidant production (using oxidant-sensitive fluorochrome dihydrorhodamine-123) monitored by intravital videomicroscopy...
Obese patients with sepsis have higher morbidity and mortality than lean counterparts, but the mechanisms involved are unknown. The authors examined inflammatory thrombogenic responses of cerebral microvasculature to induced by cecal ligation perforation in obese wild-type mice.Leukocyte platelet adhesion behavioral were measured mice 4 h postperforation. P-selectin expression different vascular beds was assessed 6 effects immunoblockade P-selectin, ICAM-1, CD18 on leukocyte recruitment...
There is growing evidence for an interplay between inflammatory and coagulation pathways in acute chronic diseases. However, it remains unclear whether components of the pathway, such as tissue factor (TF), contribute to intestinal inflammation, targeting TF will blunt cell recruitment, injury, enhanced thrombus formation that occur experimental colitis. Mice were fed 3% dextran sodium sulfate (DSS) induce colonic with some mice receiving a mouse TF-blocking antibody (muTF-Ab). The adhesion...
Emerging evidence indicates that vascular stress is an important contributor to the pathophysiology of Alzheimer's disease and related dementias (ADRD). Hydrogen sulfide (H2S) its metabolites (acid-labile (e.g., iron-sulfur clusters) bound per-, poly-) sulfides) have been shown modulate both neuronal homeostasis. We recently reported elevated plasma sulfides were associated with cognitive dysfunction measures microvascular in ADRD. Here we extend our previous work show associations between...
The influence of reductions in venular shear rate on platelet-endothelial (P/E) cell adhesion has not been previously addressed. objectives this study were to define the effects P/E and determine interdependence leukocyte-endothelial (L/E) at low rates. Intravital videomicroscopy was used quantify L/E rat mesenteric venules exposed rates ranging between 118 ± 9 835 44 s −1 . Shear altered postcapillary by rapid, graded blood withdrawal, without retransfusion shed blood. Reducing from >600...
There is abundant evidence that links hypercholesterolaemia to both vascular inflammation and atherogenesis. While atherosclerosis a large vessel disease characterized by leucocyte infiltration lipid deposition in the wall of lesion-prone arteries, inflammatory response does not appear be confined these locations. supporting systemic endothelial cell activation multiple beds appearance activated immune cells wide range mediators blood. The mechanism(s) responsible for initiating this remain...
Human inflammatory bowel diseases (IBD) are associated with significant alterations in intestinal blood flow, the direction and magnitude of which change disease progression. The objectives this study were to determine time course changes colonic perfusion that occur during development dextran-sodium-sulfate (DSS)-induced inflammation address mechanisms may underlie these flow. Intravital microscopy was used quantify flow (from measurements vessel diameter red cell velocity) different-sized...
Both leukocytes and platelets accumulate in the colonic microvasculature during experimental colitis, leading to microvascular dysfunction tissue injury. The objective of this study was determine whether recruitment inflamed venules are codependent processes. rolling adherence mice with dextran sodium sulfate (DSS)-induced colitis were monitored by intravital videomicroscopy. DSS elicited an increased both adherent platelets. DSS-colitic rendered thrombocytopenic anti-platelet serum...