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Marina Simunovic

ORCID: 0000-0002-7635-250X
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A5012281111
Research Areas
  • SARS-CoV-2 and COVID-19 Research
  • Bacteriophages and microbial interactions
  • SARS-CoV-2 detection and testing

Bielefeld University
 2021

 Multiple Occurrences of a 168-Nucleotide Deletion in SARS-CoV-2 ORF8, Unnoticed by Standard Amplicon Sequencing and Variant Calling Pipelines
OPENALEX - Publications 
David Brandt Marina Simunovic Tobias Busche Markus Haak Peter Belmann and 9 more Sebastian Jünemann Tizian Schulz Levin Joe Klages Svenja Vinke Michael Beckstette Ehmke Pohl Christiane Scherer Alexander Sczyrba Jörn Kalinowski

Genomic surveillance of the SARS-CoV-2 pandemic is crucial and mainly achieved by amplicon sequencing protocols. Overlapping tiled-amplicons are generated to establish contiguous genome sequences, which enable precise resolution infection chains outbreaks. We investigated a outbreak in local hospital used nanopore with modified ARTIC protocol employing 1200 bp long amplicons. detected deletion 168 nucleotides ORF8 gene 76 samples from outbreak. This difficult identify classical procedures...

10.3390/v13091870 article EN cc-by Viruses 2021-09-18
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