A5087211057- Effects and risks of endocrine disrupting chemicals
- Toxic Organic Pollutants Impact
- Carcinogens and Genotoxicity Assessment
- Environmental Toxicology and Ecotoxicology
- Reproductive biology and impacts on aquatic species
- Zebrafish Biomedical Research Applications
- Animal testing and alternatives
- Birth, Development, and Health
- Pharmacogenetics and Drug Metabolism
- Pharmaceutical and Antibiotic Environmental Impacts
- Drug-Induced Hepatotoxicity and Protection
- Epigenetics and DNA Methylation
- Heavy Metal Exposure and Toxicity
- Peroxisome Proliferator-Activated Receptors
- Growth Hormone and Insulin-like Growth Factors
- Agricultural safety and regulations
- Pharmacological Effects and Toxicity Studies
- Immunotoxicology and immune responses
- Pesticide Exposure and Toxicity
- Pluripotent Stem Cells Research
- Fish Ecology and Management Studies
- Per- and polyfluoroalkyl substances research
- Drug Transport and Resistance Mechanisms
- Adipose Tissue and Metabolism
- Veterinary Oncology Research
National Institute for Public Health and the Environment
2013-2024
Eindhoven Cancer Registry
2022
Maastricht University
2010-2014
University of Algarve
2005
Laboratory of Molecular Genetics
1999
Wilhelmina Children's Hospital
1997
Dutch Blood Transfusion Society
1995
Utrecht University
1994-1995
University Medical Center Utrecht
1993-1994
Heightened concern over endocrine-disrupting chemicals is driven by the hypothesis that they could reduce reproductive success and affect wildlife populations, but there little evidence for this expectation. The pharmaceutical ethynylestradiol (EE2) a potent endocrine modulator present in aquatic environment at biologically active concentrations. To investigate impacts on mechanisms of disruption, we exposed breeding populations (n = 12) zebrafish (Danio rerio) multiple generations to...
Because of its antibacterial activity nanosilver is one the most commonly used nanomaterials. It increasingly in a variety both medical and consumer products resulting an increase human exposure. However, knowledge on systemic toxicity relatively limited. To determine potential silver nanoparticles (Ag-NP) with different sizes (20 nm 100 nm) 28-days repeated dose study was performed rats using intravenous administration. The toxic effect 20 Ag-NP bench mark (BMD) approach. Treatment maximum...
The OECD validation study of the zebrafish embryo acute toxicity test (ZFET) for aquatic testing evaluated ZFET reproducibility by 20 chemicals at 5 different concentrations in 3 independent runs least laboratories. Stock solutions and were analytically confirmed 11 chemicals. Newly fertilised eggs (20/concentration control) exposed 96 h to Four apical endpoints recorded daily as indicators lethality: coagulation embryo, lack somite formation, non-detachment tail bud from yolk sac heartbeat....
The zebrafish embryotoxicity test (ZET) is a fast and simple method to study chemical toxicity after exposure of the complete vertebrate embryo during embryogenesis in ovo. We developed novel quantitative evaluation assess development based on specific endpoints time, general morphology score (GMS) system. For teratogenic effects separate scoring list was developed. relative eight glycol ethers six 1,2,4-triazole anti-fungals were evaluated this system results compared with vivo...
Environmental pollution with natural or synthetic estrogens may pose a serious threat to reproduction of wildlife species. This study describes the effects 17-alpha-ethynylestradiol (EE2) on fish reproductive organs in laboratory model. Adult zebrafish were semistatically exposed nominal concentrations 0, 10, and 25 ng/L EE2 for 24 d then transferred EE2-free medium. Gonadosomatic index (GSI), plasma vitellogenin concentration (VTG), histology gonads (control 10 only) examined as function...
A 28-day repeated dose study in rats (OECD407) enhanced for endocrine and immune parameters was performed with hexabromocyclododecane (HBCD). Rats were exposed by daily gavage to HBCD dissolved corn oil 8 groups doses ranging between 0 200 mg/kg bw per day (mkd). Evaluation consisted of dose-response analysis calculation a benchmark at the lower 95% one-sided confidence bound (BMDL) predefined critical effect sizes (CESs) 10–20%. The most remarkable findings dose-related effects on thyroid...
Nanosilver is used in a variety of medical and consumer products because its antibacterial activity. This wide application results an increased human exposure. Knowledge on the systemic toxicity nanosilver is, however, relatively scarce. In previous study, 20 nm silver nanoparticles (Ag-NP) was studied 28-day repeated-dose study rats. Ag-NP were intravenously administered with maximum dose 6 mg/kg body weight (bw)/day. Several immune parameters affected: reduced thymus weight, spleen cell...
The EU-EuroMix project adopted the strategy of European Food Safety Authority (EFSA) for cumulative risk assessment, which limits number chemicals to consider in a mixture those that induce specific toxicological phenotype. These so-called assessment groups (CAGs) are refined at several levels, including target organ and Here, we explore zebrafish embryo as test model quantitative evaluation one such CAG, skeletal malformations, through exposure compounds 0-120 hpf alcian blue cartilage...
The importance of histology as a tool in the evaluation endocrine disruption fish depends on choice and interpretation appropriate endpoints, is illustrated by analysis effects exposure to estrogen 17beta-estradiol (E2) nonaromatizable androgen 17-methyldihydrotestosterone (MDHT). E2 led disappearance vitellogenic oocytes ovary an increased area relatively large, eosinophilic cells testis, which were identified spermatogonia under high-power magnification; this was relative increase, shown...
Background: Humans are exposed to combinations of chemicals. In cumulative risk assessment (CRA), regulatory bodies such as the European Food Safety Authority consider dose addition a default and sufficiently conservative approach. The principle was confirmed previously for inducing craniofacial malformations in zebrafish embryos binary mixtures chemicals with either similar or dissimilar modes action (MOAs). Objectives: this study, we explored workflow select experimentally test multiple...
A partial life-cycle assay (PLC) with zebrafish (Danio rerio) was conducted to identify endocrine-disrupting effects of 17β-estradiol (E2) and tamoxifen (TMX) as reference for estrogen agonist antagonist activity. Adult were exposed 21 d offspring another 42 d, allowing differentiation gonads in control animals. The assessed end points included reproductive variables (egg production, fertilization, hatching), gonad juveniles, histopathology, vitellogenin (VTG) expression. With E2, the most...
Some ubiquitous pollutants of the aquatic environment, such as PCBs or other polyhalogenated aromatic hydrocarbons, may disrupt thyroid hormone system. In a partial life cycle assay with zebrafish (Danio rerio), we studied effects reference compound propylthiouracil (PTU) on reproduction, growth and development, histopathology some target tissues, plasma levels. PTU induced concentration-dependent increase egg production concomitant decrease mature oocyte size but had no effect fertilization...
We studied the presence of hydroxylated metabolites hexabromocyclododecane (HBCD) in three wildlife species (tern egg, seal, and flounder) Wistar rats exposed to 30 100 mg HBCD/kg bw/day for 28 days. A nondestructive extraction, fractionation, cleanup method was developed separate HBCD from biotic sample matrix. Four different groups were identified rat adipose, liver, lung, muscle tissues by liquid gas chromatography (LC GC) combined with mass spectrometry (MS): monohydroxy HBCD,...