A5065365955- MicroRNA in disease regulation
- RNA Research and Splicing
- RNA Interference and Gene Delivery
- Chromosomal and Genetic Variations
- Insect Resistance and Genetics
- Circular RNAs in diseases
- CRISPR and Genetic Engineering
- Advanced Polymer Synthesis and Characterization
- Cancer-related molecular mechanisms research
- Advanced biosensing and bioanalysis techniques
- Dendrimers and Hyperbranched Polymers
- Plant Virus Research Studies
- RNA modifications and cancer
- Nanoparticle-Based Drug Delivery
- Luminescence and Fluorescent Materials
- Conducting polymers and applications
- Nanoplatforms for cancer theranostics
- Marine Ecology and Invasive Species
- Protein Interaction Studies and Fluorescence Analysis
- RNA and protein synthesis mechanisms
- Genomics and Phylogenetic Studies
- Marine Biology and Environmental Chemistry
- Extracellular vesicles in disease
- Genetics, Aging, and Longevity in Model Organisms
- Developmental Biology and Gene Regulation
University of Southern Mississippi
2016-2025
Kettering University
2008-2017
Hattiesburg Clinic
2014-2016
Vanderbilt University
2004-2010
Memorial Sloan Kettering Cancer Center
2010
University of Sheffield
2008
Atypical miRNA substrates do not fit criteria often used to annotate canonical miRNAs, and can escape the notice of genefinders. Recent analyses expanded catalogs invertebrate splicing-derived miRNAs (“mirtrons”), but only a few tens mammalian mirtrons have been recognized date. We performed meta-analysis 737 mouse human small RNA data sets comprising 2.83 billion raw reads. Using strict conservative criteria, we provide confident annotation for 237 240 vast majority which are novel genes....
RNAi-based technologies are ideal for pest control as they can provide species specificity and spare nontarget organisms. However, in some pests biological barriers prevent use of RNAi, therefore broad application. In this study we tested the ability a synthetic cationic polymer, poly-[N-(3-guanidinopropyl)methacrylamide] (pGPMA), that mimics arginine-rich cell penetrating peptides to trigger RNAi an insensitive animal—Spodoptera frugiperda. Polymer–dsRNA interpolyelectrolyte complexes...
Plants live in association with microorganisms that positively influence plant development, vigor, and fitness response to pathogens abiotic stressors. The bulk of the microbiome is concentrated belowground at root-soil interface. Plant roots secrete carbon-rich rhizodeposits containing primary secondary low molecular weight metabolites, lysates, mucilages. These exudates provide nutrients for soil modulate their affinity host plants, but details this process are largely unresolved. We...
MicroRNAs (miRNAs) are highly conserved small RNAs that act as translational regulators of gene expression, exerting their influence by selectively targeting mRNAs bearing complementary sequence elements. These function in diverse aspects animal development and physiology. Because an ability to rapid responders at the level translation, miRNAs may also stress response. In this study, we show miR-8 family regulates osmoregulation zebrafish embryos. Ionocytes, which a specialized cell type...
Previous studies revealed roles for RNA interference (RNAi) in the immediate cellular response to viral infection plants, nematodes and flies. However, little is known about how RNAi combats viruses during persistent or latent infections. Our analysis of small RNAs cloned from Drosophila cells latently infected with Flock House Virus (FHV) failed reveal signatures bulk degradation genome. Instead, this + strand virus specifically generated Dicer-2-dependent, 21-nucleotide siRNAs that derived...
Rod-like structures composed of actin and the actin-binding protein cofilin are found in Alzheimer's disease (AD) patients. However, mechanisms underlying formation these their pathological consequences still largely unknown. We that microRNAs 103 107 repress translation cofilin, reduced levels miR-103 or miR-107 associated with elevated rod-like a transgenic mouse model AD. These results suggest may play an important role cytoskeletal pathology
Organic dyes that absorb and emit in the near-infrared (NIR) region are potentially noninvasive, high-resolution, rapid biological imaging materials. Indolizine donor-based cyanine squaraine with water-solubilizing sulfonate groups were targeted this study due to strong absorptions emissions NIR region. As previously observed for nonwater-soluble derivatives, indolizine group retains a substantial shift toward longer wavelengths both absorption emission squaraines cyanines relative...
Postpolymerization modification of highly defined "scaffold" polymers is a promising approach for overcoming the existing limitations controlled radical polymerization such as batch-to-batch inconsistencies, accessibility to different monomers, and compatibility with harsh synthesis conditions. Using multiple physicochemical characterization techniques, we demonstrate that poly(2-vinyl-4,4-dimethyl azlactone) (PVDMA) scaffolds can be efficiently modified coumarin derivative, doxorubicin,...
Abstract The oncogene DJ-1 has been associated with multiple cancers, including prostate cancer, where it can be stabilized by androgens and antiandrogens. However, little data exist on the expression pattern function of in cancer. To address prostate, a yeast two-hybrid screen was done to identify novel binding proteins. androgen receptor (AR) identified confirmed as partner. This is first evidence that directly interacts AR. We also show modulation regulated AR transcriptional activity....
We expanded the knowledge base for Drosophila cell line transcriptomes by deeply sequencing their small RNAs. In total, we analyzed more than 1 billion raw reads from 53 libraries across 25 lines. verify reproducibility of biological replicate data sets, determine common and distinct aspects miRNA expression lines, infer global impact miRNAs on transcriptomes. next characterize commonalities differences in endo-siRNA populations. Interestingly, most lines exhibit enhanced TE-siRNA production...
Thiol–ene photopolymerization in miniemulsion under non-stoichiometric conditions offers a facile route to functionalized polymer nanoparticles.
The propensity of animal miRNAs to regulate targets bearing modest complementarity, most notably via pairing with miRNA positions ∼2-8 (the "seed"), is believed drive major aspects evolution. First, minimal targeting requirements have allowed conserved acquire large target cohorts, thus imposing strong selection on maintain their seed sequences. Second, the needed for repression suggests that evolutionarily nascent may generally induce net detrimental, rather than beneficial, regulatory...
Linear-dendritic block copolymers (LDBCs) have emerged as promising materials for drug delivery applications, with their hybrid structure exploiting advantageous properties of both linear and dendritic polymers. LDBCs encapsulation efficiencies that can be used to encapsulate hydrophobic hydrophilic dyes bioimaging, cancer therapeutics, small biomolecules. Additionally, LDBCS readily functionalized varying terminal groups more efficient targeted delivery. However, depending on structural...
Abstract microRNAs (miRNAs) are small (∼22 nucleotide) non‐coding RNAs that regulate gene expression at the post‐transcriptional level, typically by inhibiting translation. The genes encoding these estimated to comprise approximately 2–3% of animal genomes yet potentially a majority protein‐coding including those involved in cell specification and development. A key remaining question is identify target mRNAs regulated microRNAs. As means potential targets, we designed sensitive microarray...
To assess miRNA evolution across the Drosophila genus, we analyzed several billion small RNA reads 12 fruit fly species. These data permit comprehensive curation of species- and clade-specific variation in identity, abundance, processing. Among well-conserved miRNAs, observed unexpected cases 5′ end precision, occasional antisense loci, putatively noncanonical loci. We also used strict criteria to identify a large set (649) novel, evolutionarily restricted miRNAs. Within bulk collection...
House dust mites are common pests with an unusual evolutionary history, being descendants of a parasitic ancestor. Transition to parasitism is frequently accompanied by genome rearrangements, possibly accommodate the genetic change needed access new ecology. Transposable element (TE) activity source genomic instability that can trigger large-scale alterations. Eukaryotes have multiple transposon control mechanisms, one which RNA interference (RNAi). Investigation mite failed identify major...
A small percentage of babies born to Zika virus (ZIKV)-infected mothers manifest severe defects at birth, including microcephaly. Among those who appeared healthy there are increasing reports postnatal growth or developmental defects. However, the impact congenital ZIKV infection in development is poorly understood. Here, we report that a mild ZIKV-infection pups immunocompetent pregnant mice did not display apparent but manifested impediments and neurobehavioral deficits, which include...
ABSTRACT Bats are reservoirs for numerous viruses that cause serious diseases in other animals and humans. Several mechanisms proposed to contribute the tolerance of bats these pathogens. This study investigates response bat cells double-stranded RNA generated by SARS-CoV-2 replication. Here, we found involvement Dicer processing viral genomic RNAs during infection. Examining sequencing infected cells, small-interfering (siRNA)-like fragments were derived from RNAs. Depletion showed a...
Bats are reservoirs for numerous viruses that cause serious diseases in other animals and humans. Several mechanisms proposed to contribute the tolerance of bats these pathogens. This study investigates response bat cells double-stranded RNA generated by SARS-CoV-2 replication. Here, we found involvement Dicer processing viral genomic RNAs during infection. Examining sequencing infected cells, small-interfering (siRNA)-like fragments were derived from RNAs. Depletion showed a reduction an...