Joana P. Firmino

ORCID: 0000-0002-7694-0416
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About
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Research Areas
  • Agricultural safety and regulations
  • Aquaculture disease management and microbiota
  • Aquaculture Nutrition and Growth
  • Pesticide Residue Analysis and Safety
  • Genetically Modified Organisms Research
  • Pharmaceutical Economics and Policy
  • Invertebrate Immune Response Mechanisms
  • Pharmaceutical studies and practices
  • Animal Genetics and Reproduction
  • Protein Hydrolysis and Bioactive Peptides
  • Vitamin K Research Studies
  • Antimicrobial Peptides and Activities
  • Insect Utilization and Effects
  • Effects and risks of endocrine disrupting chemicals
  • Moringa oleifera research and applications
  • Vibrio bacteria research studies
  • Parasite Biology and Host Interactions
  • Natural product bioactivities and synthesis
  • Biochemical Analysis and Sensing Techniques
  • Intellectual Property and Patents
  • Date Palm Research Studies
  • Marine Bivalve and Aquaculture Studies
  • Bioactive Compounds and Antitumor Agents
  • Reproductive biology and impacts on aquatic species
  • Pharmacological Effects and Assays

Institute of Agrifood Research and Technology
2018-2023

Universitat Autònoma de Barcelona
2019-2021

Faes Farma (Spain)
2021

Junta de Andalucía
2017-2018

Andalusian Institute of Agricultural and Fisheries Research and Training
2017-2018

Essential oils (EOs) are promising alternatives to chemotherapeutics in animal production due their immunostimulant, antimicrobial, and antioxidant properties, without associated environmental or hazardous side effects. In the present study, modulation of transcriptional immune response (microarray analysis) microbiota [16S Ribosomal RNA (rRNA) sequencing] intestine euryhaline fish gilthead seabream (Sparus aurata) fed a dietary supplementation garlic, carvacrol, thymol EOs was evaluated....

10.3389/fimmu.2021.625297 article EN cc-by Frontiers in Immunology 2021-03-04

One of the main targets for use phytogenics in aquafeeds is mucosal tissues as they constitute a physical and biochemical shield against environmental pathogenic threats, comprising elements from both innate acquired immunity. In present study, modulation skin transcriptional immune response, bacterial growth capacity mucus, overall health condition gilthead seabream ( Sparus aurata ) juveniles fed dietary supplementation garlic essential oil, carvacrol, thymol were assessed. The enrichment...

10.3389/fimmu.2021.633621 article EN cc-by Frontiers in Immunology 2021-03-12

Abstract Following a request from the European Commission, EFSA was asked to deliver scientific opinion on safety and efficacy of Lactiplantibacillus plantarum NCIMB 30094 as technological additive (functional group: silage additive) for use in fresh material all animal species. The is intended at proposed minimum concentration 1 × 109 colony forming units (CFU)/kg plant material. bacterial species considered by be suitable qualified presumption approach assessment. identity strain...

10.2903/j.efsa.2025.9144 article EN cc-by-nd EFSA Journal 2025-01-01

Abstract Following a request from the European Commission, EFSA was asked to deliver scientific opinion on assessment of application for renewal authorisation l‐valine produced by fermentation with genetically modified strain Corynebacterium glutamicum KCCM 80058 as nutritional feed additive all animal species. The is authorised use in species (3c370). applicant has provided evidence that currently market complies existing conditions authorisation. Panel Additives and Products or Substances...

10.2903/j.efsa.2025.9251 article EN cc-by-nd EFSA Journal 2025-02-01

Following a request from the European Commission, EFSA was asked to deliver scientific opinion on assessment of application for renewal authorisation choline chloride as nutritional additive all animal species. The is already authorised use in species (3a890). applicant provided evidence that currently market complies with existing conditions authorisation. Panel Additives and Products or Substances used Animal Feed (FEEDAP) concluded feed nutrition under proposed remains safe target...

10.2903/j.efsa.2025.9264 article EN cc-by-nd EFSA Journal 2025-02-01

Abstract Following a request from the European Commission, Food Safety Authority (EFSA) was asked to deliver scientific opinion on safety and efficacy of l‐valine produced with genetically modified strain Corynebacterium glutamicum (KCCM 80365) when used as nutritional additive in feed water for drinking all animal species categories. l‐Valine manufactured by fermentation using C. KCCM 80365 does not give rise any concern regarding genetic modifications production strain. No viable cells or...

10.2903/j.efsa.2025.9348 article EN cc-by-nd EFSA Journal 2025-04-01

Abstract Following a request from the European Commission, EFSA was asked to deliver scientific opinion on safety and efficacy of feed additive consisting l‐tryptophan produced by fermentation with genetically modified strain Corynebacterium glutamicum (KCCM 80346) when used as nutritional in water for drinking all animal species categories. Viable cells production its DNA were not detected additive. l‐Tryptophan manufactured using C. KCCM 80346 does give rise any concern regard genetic...

10.2903/j.efsa.2025.9327 article EN cc-by-nd EFSA Journal 2025-04-01

Abstract Following a request from the European Commission, Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) was asked to deliver scientific opinion L‐lysine sulfate produced by fermentation with genetically modified strain of Corynebacterium glutamicum (CGMCC 7.453) when as nutritional additive feed for all animal species. The active substance is L‐lysine. FEEDAP concluded that production qualifies qualified presumption safety (QPS) approach assessment; therefore,...

10.2903/j.efsa.2025.9343 article EN cc-by-nd EFSA Journal 2025-04-01

A microencapsulated feed additive composed by garlic, carvacrol and thymol essential oils (EOs) was evaluated regarding its protective effect in gills parasitized Sparicotyle chrysophrii Sparus aurata. nutritional trial (65 days) followed a cohabitation challenge with fish (39 were performed. Transcriptomic analysis microarrays of fed the EOs diet showed an up-regulation genes related to biogenesis, vesicular transport exocytosis, leukocyte-mediated immunity, oxidation-reduction overall...

10.1038/s41598-020-74625-5 article EN cc-by Scientific Reports 2020-10-20

From a general structural perspective, mucosal tissue is constituted by two main matrices: the and secreted mucus. Jointly, they fulfill wide range of functions including protection epithelial layer. In this study, we simultaneously analyzed mucus response using holistic interactome-based multi-omics approach. The effect gilthead sea bream ( Sparus aurata ) skin mucosa to dietary inclusion spray-dried porcine plasma (SDPP) was evaluated. microarrays-based transcriptome data showed 194...

10.3389/fimmu.2020.613824 article EN cc-by Frontiers in Immunology 2021-02-04

Following a request from the European Commission, EFSA was asked to deliver scientific opinion on safety of 41 compounds provide Herbal flavour and belonging different chemical groups, when used as sensory additives in feed for all animal species. Fourteen out were tested tolerance studies chickens fattening, piglets, cattle fattening Atlantic salmon. No adverse effects observed at 10-fold intended level. The Panel Additives Products or Substances Animal Feed (FEEDAP) concluded that 14 safe...

10.2903/j.efsa.2023.8340 article EN cc-by-nd EFSA Journal 2023-10-01

Abstract Background The development of a sustainable business model with social acceptance, makes necessary to develop new strategies guarantee the growth, health, and well-being farmed animals. Debaryomyces hansenii is yeast species that can be used as probiotic in aquaculture due its capacity i) promote cell proliferation differentiation, ii) have immunostimulatory effects, iii) modulate gut microbiota, and/or iv) enhance digestive function. To provide inside into effects D. on juveniles...

10.1186/s40104-023-00895-4 article EN cc-by Journal of Animal Science and Biotechnology/Journal of animal science and biotechnology 2023-07-09

Abstract Following a request from the European Commission, EFSA Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) was asked to deliver scientific opinion safety efficacy of 6-phytase produced by genetically modified strain Aspergillus oryzae DSM 33737 (HiPhorius™ 10, 40, 20L 50L) as zootechnical feed additive for all poultry, Suidae fin fish. The FEEDAP concluded that genetic modification production does not give rise concerns. Based no observed adverse effect level...

10.2903/j.efsa.2024.8663 article EN cc-by-nd EFSA Journal 2024-03-01

The inclusion of a medicinal plant leaf extract (MPLE) from sage ( Salvia officinalis ) and lemon verbena Lippia citriodora ), rich in verbascoside triterpenic compounds like ursolic acid, was evaluated gilthead seabream Sparus aurata fed low fishmeal-based diet (48% crude protein, 17% fat, 21.7 MJ kg -1 , 7% fishmeal, 15% fish oil) for 92 days. In particular, the study focused on effect these phytogenic gut condition by analyzing transcriptomic profiling (microarray analysis) histological...

10.3389/fimmu.2021.670279 article EN cc-by Frontiers in Immunology 2021-05-14

Abstract Background The identification of DNA methyltransferases (Dnmt) expression patterns during development and their regulation is important to understand the epigenetic mechanisms that modulate larval plasticity in marine fish. In this study, dnmt1 dnmt3 paralogs were identified flatfish Solea senegalensis early developmental stages juveniles determined. Additionally, Dnmt transcription by a specific inhibitor (5-aza-2′-deoxycytidine) temperature was evaluated. Results Five paralog...

10.1186/s12861-017-0154-0 article EN cc-by BMC Developmental Biology 2017-07-17

A nutritional study was conducted to evaluate the inclusion of green microalga Scenedesmus sp. at 5% (SCE-5) as an alternative fishmeal ingredient. This tested with four replicates during 45 days using isolipidic (18%), isoproteic (48%), and isoenergetic (1.9 MJ kg−1) diets. Fish fed showed similar growth feed efficiency parameters control group. Regarding digestive function, SCE-5 diet enhanced activity alkaline pancreatic proteases, whereas it did not affect that intestinal enzymes...

10.3390/ani10091656 article EN cc-by Animals 2020-09-15

The effect of spray-dried porcine plasma (SDPP) on the intestinal histological organization and autochthonous microbiota composition was evaluated in Sparus aurata. Fish were fed a basal diet (51 g/kg protein, 17 fat, 20.6 MJ/kg gross energy) containing 3 SDPP for 95 days (initial body weight, BW = 9.5 ± 0.2g, mean SD). inclusion promoted growth (p < .05), being fish 6.2% (BW 88.2 1.6 g) heavier than control 82.7 3.2 g). increased density goblet cells whereas no differences villi height...

10.1111/anu.13039 article EN Aquaculture Nutrition 2020-01-24

The effects of porcine plasma protein hydrolysate (PPH) on growth, feed efficiency, and immune responses was evaluated in Sparus aurata. Fish were fed two isoproteic (48% protein), isolipidic (17% fat), isoenergetic diets (21.7 MJ/kg) diets, one them containing 5% PPH at the expense fishmeal. Both tested for 92 days. A significant increase growth observed fish diet comparison to control group (182.2 ± 4.4 vs. 173.8 4.1 g), as well an intake without worsening FCR values. An ex vivo assay,...

10.3390/ani11072122 article EN cc-by Animals 2021-07-16

Tools are required for quick and easy preliminary evaluation of functional feeds efficiency on fisheries. The analysis skin mucus biomarkers is a recent alternative approach providing faster feed-back from the laboratory which characterized by being less invasive, more rapid with reduced costs. effect replacing fishmeal fish protein hydrolysates means two porcine by-products, spray-dried plasma (SDPP) pig hydrolysate (PPH), in compound diets (50.4% crude protein, 16.2% 22.1 MJ/kg feed) was...

10.1016/j.fsi.2021.09.011 article EN cc-by-nc-nd Fish & Shellfish Immunology 2021-09-14
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