Manuel Manchado

ORCID: 0000-0002-4584-4878
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About
Contact & Profiles
Research Areas
  • Aquaculture Nutrition and Growth
  • Aquaculture disease management and microbiota
  • Identification and Quantification in Food
  • Reproductive biology and impacts on aquatic species
  • Physiological and biochemical adaptations
  • Fish Biology and Ecology Studies
  • Genetic diversity and population structure
  • Genomics and Phylogenetic Studies
  • Animal Genetics and Reproduction
  • Invertebrate Immune Response Mechanisms
  • Chromosomal and Genetic Variations
  • Fish Ecology and Management Studies
  • Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
  • Genetic and phenotypic traits in livestock
  • Ichthyology and Marine Biology
  • Meat and Animal Product Quality
  • Marine Bivalve and Aquaculture Studies
  • Marine and coastal ecosystems
  • interferon and immune responses
  • RNA Research and Splicing
  • MicroRNA in disease regulation
  • Cholesterol and Lipid Metabolism
  • RNA Interference and Gene Delivery
  • Advanced biosensing and bioanalysis techniques
  • Retinoids in leukemia and cellular processes

Junta de Andalucía
2015-2025

Andalusian Institute of Agricultural and Fisheries Research and Training
2015-2025

Consejería de Agricultura, Pesca, Agua y Desarrollo Rural
2006-2020

International Centre for Advanced Mediterranean Agronomic Studies
2019

Universidad de Málaga
2015-2016

Niğde Ömer Halisdemir Üniversitesi
2011

Harran University
2011

Ankara University
2011

Instituto Federal do Amapá
2010

Universidad de Cádiz
2006

Flatfish metamorphosis involves major physiological and morphological changes. Due to its importance in aquaculture as a model for developmental studies, some gene expression studies have focused on the understanding of this process using quantitative real-time PCR (qRT-PCR) technique. Therefore, adequate reference genes accurate normalization are required. The stability 12 potential was examined during larval development Senegalese sole (Solea senegalensis) Atlantic halibut (Hippoglossus...

10.1186/1471-2199-9-28 article EN cc-by BMC Molecular Biology 2008-01-01

Senegalese sole (Solea senegalensis) and common (S. solea) are two economically evolutionary important flatfish species both in fisheries aquaculture. Although some genomic resources tools were recently described these species, further sequencing efforts required to establish a complete transcriptome, identify new molecular markers. Moreover, the comparative analysis of transcriptomes will be useful understand evolution. A comprehensive characterization transcriptome for each was carried out...

10.1186/1471-2164-15-952 article EN cc-by BMC Genomics 2014-11-03

The aim of this study was to determine the influence feeding regimes in Senegalese sole (Solea senegalensis) cultured under extensive, semi-extensive and intensive production systems. A total 254 bacterial isolates from guts fish different systems were tested. Biochemical tests genetic analyses based on 16S rDNA sequence analysis conduced identify strains. Vibrio species most represented taxonomic group culturable microbiota S. senegalensis Particularly, ichthyoenteri frequently isolated...

10.1111/j.1365-2109.2007.01790.x article EN Aquaculture Research 2007-08-01

The Senegalese sole, Solea senegalensis, is a highly prized flatfish of growing commercial interest for aquaculture in Southern Europe. However, despite the industrial production sole being hampered primarily by lack information on physiological mechanisms involved reproduction, growth and immunity, very limited genomic available this species. Sequencing S. senegalensis multi-tissue normalized cDNA library, from adult tissues (brain, stomach, intestine, liver, ovary, testis), larval stages...

10.1186/1471-2164-9-508 article EN cc-by BMC Genomics 2008-01-01

ABSTRACT Simultaneous expression of seven genes in Escherichia coli was measured by a reverse transcription-multiplex PCR fluorescence procedure. Genes studied were (i) oxyR (transcriptional regulator); (ii) katG , dps gorA and ahpCF (controlled OxyR); (iii) sodA SoxRS); (iv) trxA (not related to OxyR or SoxRS). Except for transcription all activated during the course growth wild-type bacteria, though notable variations observed with respect both time extent activation. Whereas exponential...

10.1128/jb.181.9.2759-2764.1999 article EN Journal of Bacteriology 1999-05-01

10.1007/s12263-012-0312-8 article EN Genes & Nutrition 2012-08-18

Abstract Flatfish metamorphosis is a unique post-embryonic developmental event in which thyroid hormones (THs) drive the development of symmetric pelagic larva into asymmetric benthic juveniles. One eyes migrates to join other eye on opposite side head. Developmental mechanisms at basis acquisition flatfish anatomical asymmetry remain an open question. Here we demonstrate that TH responsive centre, determined by deiodinase 2 expression, ventrally juxtaposed migrating sole ( Solea...

10.1038/s41598-018-29957-8 article EN cc-by Scientific Reports 2018-08-10

Superoxide dismutase-rich Tetraselmis chuii (T. chuii) is derived from marine microalgae and has been reported to increase gene expression of nuclear factor erythroid 2-related 2 (NRF2) related antioxidant enzymes in myoblast tissue culture models. Human research indicated that T. supplementation can improve recovery exercise-induced muscle damage, but its effects on endurance exercise performance the molecular bases may underlie any ergogenic are unclear. Healthy participants underwent 14...

10.3390/antiox14040435 article EN cc-by Antioxidants 2025-04-03
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