- Aquaculture disease management and microbiota
- Parasite Biology and Host Interactions
- Marine Bivalve and Aquaculture Studies
- Aquaculture Nutrition and Growth
- Aquatic Invertebrate Ecology and Behavior
- Reproductive biology and impacts on aquatic species
- Vibrio bacteria research studies
- Microbial infections and disease research
- Parasitic Infections and Diagnostics
- Crustacean biology and ecology
- Environmental Toxicology and Ecotoxicology
- Marine and fisheries research
- Myxozoan Parasites in Aquatic Species
- Microbial Community Ecology and Physiology
- Genomics and Phylogenetic Studies
- Identification and Quantification in Food
- Invertebrate Immune Response Mechanisms
- Gut microbiota and health
- Toxic Organic Pollutants Impact
- Pharmaceutical and Antibiotic Environmental Impacts
- Fish Ecology and Management Studies
- Heavy metals in environment
- Parasites and Host Interactions
- Marine Ecology and Invasive Species
- Health and Medical Research Impacts
Junta de Andalucía
2010-2022
Andalusian Institute of Agricultural and Fisheries Research and Training
2011-2022
Centro Tecnológico del Agua
2009-2022
Instituto Nacional del Agua
2019
University of California, San Diego
2017-2019
Consejería de Agricultura, Pesca, Agua y Desarrollo Rural
1992-2018
Laboratoire des Sciences de l'Environnement Marin
2014
Ifremer
2014
Universidad de Granada
2008
University of Córdoba
1994-1995
Five Gram-negative bacterial isolates, recovered from an outbreak that occurred in March 2006 Huelva, Spain, affecting adult diseased cultured wedge sole [ Dicologlossa cuneata (Moreau)], were characterized phenotypically and genotypically order to clarify their taxonomic position. On the basis of 16S rRNA gene sequence analysis, isolates included genus Pseudomonas , within fluorescens -related species group, closest relatives being jessenii koreensis subgroups. The highest similarities...
A mass mortality event (MME) affecting the fan mussel Pinna nobilis was first detected in Spain autumn 2016 and spread north- eastward through Mediterranean Sea. Various pathogens have been blamed for contributing to MME, with emphasis Haplosporidium pinnae , Mycobacterium sp. Vibrio spp. In this study, samples from 762 mussels (necropsies 263 individuals, mantle biopsies 499) of various health conditions, wide geographic age range, taken before during MME environments along Sea, were used...
Abstract Three species of marine bivalve molluscs ( Chamelea gallina, Ruditapes decussatus , and Crassostrea gigas ) have been studied in order to evaluate the levels pollution on South Atlantic Spanish littoral. Several transition metals (Cu, As, Cd, Sn, Hg, Pb) were determined as a general index total contamination. Animals from putative contaminated areas exhibited higher metal contents than those cleaner waters. C. showed 5–20‐fold content other two species. The mutagenicity ethanolic...
The osmoregulatory responses of 20 days acclimation to environmental salinities 5‰, 15‰, 25‰, 35‰ and 55‰ were assessed in juveniles wedge sole (Dicologoglossa cuneata Moreau, 1881). This shows a good capacity adapt this range salinities. A direct linear relationship between salinity plasma osmolality was observed, with calculated isosmotic point 10.4‰ (284 mOsm kg−1). Na+, K+-ATPase activity the gills followed ‘U-shaped’ salinity, kidney tissue. Plasma cortisol levels elevated fish held...
Marteilia refringens is a paramyxean parasite which infects the flat oyster Ostrea edulis and mussels (Mytilus galloprovincialis), where it has been attributed to separate species, maurini, by several authors. Doubts persist though as existence or not of two species in Europe. We have devised molecular method for diagnosis M. based on 358 bp nested-PCR rDNA intergene spacer (rDNA IGS) capable detecting 0·5 fg DNA. Molecular characterization this indicates that parasites infect oysters are...
We introduce two concepts to quantify the novelty of a microbiome. The first, microbiome score (MNS), allows identification microbiomes that are especially different from what is already sequenced. second, attention (MAS), have many close neighbors, implying considerable scientific devoted their study. By computing focus index based on MNS and MAS, we objectively track compare scores individual samples projects over time predict future trends in field; i.e., work toward yielding...
Physiological responses to a high stocking density were tested in juvenile wedge sole Dicologoglossa cuneata (Moreau). Fish kept at low (1 kg m−2), medium (3 m−2) and (9 densities for 22 days. No differences the weight, length, survival hepatosomatic index observed among treatments. Basal plasma cortisol osmolality found be directly positively related density. A mild increase glucose was seen density, protein elevated densities. The liver glycogen content inversely triglyceride level...
Bonamia ostreae and B. exitiosa have caused mass mortalities of various oyster species around the world co-occur in some European areas. The World Organisation for Animal Health (OIE) has included infections with both list notifiable diseases. However, official methods species-specific diagnosis either parasite certain limitations. In this study, new conventional PCR (cPCR) real-time techniques were developed to diagnose each species. Moreover, a multiplex method was designed detect...
Noble pen shell or fan mussel, Pinna nobilis Linnaeus (1758), protected since 1992, was incorporated into the Spanish Catalogue of Threatened Species (Category: Vulnerable, Royal Decree 139/2011). The status is presently in process being catalogued as critically endangered, pending approval by Government (https://www.mapama.gob.es/es/biodiversidad/participacion-publica/Borrador_OM_situacion_critica.aspx). International Union for Conservation Nature (IUCN) alerted countries Mediterranean...
Due to their widespread distribution and virulence, protozoan species of the genus Perkinsus are especially worrisome parasites for shellfish farmers. In present paper, we investigate organization structural features nuclear ribosomal genes atlanticus as well use DNA sequence information from this region phylogenetic analyses. This has been useful, further, development a diagnostic test based on amplification by polymerase chain reaction (PCR) technique. We have isolated high-copy in...
In the course of a histopathological survey performed to discover cause mass mortality striped clam Chamelea gallina in Balearic Islands (Spain, Mediterranean Sea), we detected Marteilia-like parasite 3 clams. Molecular methods were applied identify parasite. DNA extracted from paraffin block was used carry out PCR assay for Marteilia refringens detection based on rDNA sequence (the intergenic spacer ribosomal genes, IGS). The nucleotide IGS amplified fragment and positive signal obtained by...
Abstract Hepatitis A virus (HAV) infection is highly prevalent in Latin America, including Venezuela. Subgenotype IA seems to circulate an almost exclusive fashion, except Brazil. The aim of this study was the molecular characterization HAV Venezuela, order characterize circulating strains and analyze presence quasispecies sporadic cases epidemic outbreak. total 125 (113 sera 12 feces) samples positive for anti‐HAV IgM from outbreak, were submitted hemi‐nested RT‐PCR amplification VP1 N...