A5051452804- Plant nutrient uptake and metabolism
- Plant Molecular Biology Research
- Plant Stress Responses and Tolerance
- Plant tissue culture and regeneration
- Photosynthetic Processes and Mechanisms
- Plant responses to water stress
- Biochemical and Molecular Research
- Rice Cultivation and Yield Improvement
- Isotope Analysis in Ecology
- RNA modifications and cancer
- Lipid metabolism and biosynthesis
- Plant Reproductive Biology
- Polysaccharides and Plant Cell Walls
- Plant Parasitism and Resistance
- Seed Germination and Physiology
- Transgenic Plants and Applications
- Spectroscopy Techniques in Biomedical and Chemical Research
- Aluminum toxicity and tolerance in plants and animals
- Metabolomics and Mass Spectrometry Studies
- Metabolism and Genetic Disorders
- Spectroscopy and Chemometric Analyses
- Plant Micronutrient Interactions and Effects
- Legume Nitrogen Fixing Symbiosis
Leibniz Institute of Plant Genetics and Crop Plant Research
2017-2024
Adventitious root (AR) formation in excised plant parts is a bottleneck for survival of isolated fragments. AR plays an important ecological role and critical process cuttings the clonal propagation horticultural forestry crops. Therefore, understanding regulation excision-induced essential sustainable efficient utilization genetic resources.Recent studies transcriptomes, proteomes metabolomes, use mutants transgenic lines have significantly expanded our knowledge concerning formation. Here,...
Plants undergo several developmental transitions during their life cycle. One of these, the differentiation young embryo from a meristem-like structure into highly specialized storage organ, is believed to be controlled by local connections between sugars and hormonal response systems. However, we know little about regulatory networks underpinning sugar-hormone interactions in developing seeds. By modulating trehalose 6-phosphate (T6P) content growing embryos garden pea (Pisum sativum),...
Abstract Even though Sugars Will Eventually be Exported Transporters (SWEETs) have been found in every sequenced plant genome, a comprehensive understanding of their functionality is lacking. In this study, we focused on the SWEET family barley (Hordeum vulgare). A radiotracer assay revealed that expressing HvSWEET11b African clawed frog (Xenopus laevis) oocytes facilitated bidirectional transfer not only just sucrose and glucose, but also cytokinin. Barley plants harboring loss-of-function...
Abstract Maize (Zea mays) kernels are the largest cereal grains, and their endosperm is severely oxygen deficient during grain fill. The causes, dynamics, mechanisms of acclimation to hypoxia minimally understood. Here, we demonstrate that develops in small, growing endosperm, but not nucellus, becomes standard state, regardless diverse structural genetic perturbations modern maize (B73, popcorn, sweet corn), mutants (sweet4c, glossy6, waxy), non-domesticated wild relatives (teosintes...
Adventitious root (AR) formation is characterized by a sequence of physiological and morphological processes determined external factors, including mineral nutrition, the impacts which remain largely elusive. Morphological anatomical evaluation effects elements on AR in leafy cuttings Petunia hybrida revealed striking stimulation iron (Fe) promotive action ammonium (NH4+). The optimal application period for these nutrients corresponded to early division meristematic cells rooting zone...
The adaptation strategies of halophytic seaside barley Hordeum marinum to high salinity and osmotic stress were investigated by nuclear magnetic resonance imaging, as well ionomic, metabolomic, transcriptomic approaches. When compared with cultivated barley, exhibited a better plant growth rate, higher relative water content, lower pressure, sustained photosynthetic activity under salinity, but not stress. As is capable controlling Na+ Cl− concentrations in leaves at the roots appear play...
Root growth in maize (Zea mays L.) is regulated by the activity of quiescent center (QC) stem cells located within root apical meristem. Here, we show that despite being highly hypoxic under normal oxygen tension, QC are vulnerable to stress, which causes their degradation with subsequent inhibition growth. Under low oxygen, became depleted starch and soluble sugars exhibited reliance on glycolytic fermentation impairment TCA cycle through depressed several enzymes, including pyruvate...
Abstract Cereal grains contribute substantially to the human diet. The maternal plant provides carbohydrate and nitrogen sources deposited in endosperm, but basis for their spatial allocation during grain filling process is obscure. Here, vacuolar processing enzymes have been shown both mediate programmed cell death (PCD) tissues of a barley influence delivery assimilate endosperm. proposed centrality PCD has implications cereal crop improvement.
Nucleotide limitation and imbalance is a well-described phenomenon in animal research but understudied the plant field. A peculiarity of pyrimidine de novo synthesis plants complex subcellular organization. Here, we studied two organellar localized enzymes pathway, with chloroplast aspartate transcarbamoylase (ATC) mitochondrial dihydroorotate dehydrogenase (DHODH). ATC knock-downs were most severely affected, exhibiting low levels nucleotides, energy state, reduced photosynthetic capacity...
Summary The initial free expansion of the embryo within a seed is at some point inhibited by its contact with testa, resulting in formation folds and borders. Although less obvious, mechanical forces appear to trigger accelerate maturation. However, mechanistic basis for this effect remains unclear. Manipulation constraints affecting either vivo or vitro growth oilseed rape embryos was combined analytical approaches, including magnetic resonance imaging computer graphic reconstruction,...
Abstract Plants undergo several developmental transitions during their life cycle. One of these, the differentiation young embryo from a meristem-like structure into highly-specialized storage organ, is vital to formation viable seed. For crops in which seed itself end product, effective accumulation compounds economic relevance, defining quantity and nutritive value harvest yield. However, regulatory networks underpinning phase transition filling are poorly understood. Here we show that...
Fingerprint analysis is a common technique in forensic and criminal investigations. Similar techniques exist the field of infrared spectroscopy to identify biomolecules according their characteristic spectral fingerprint features. These unique markers are located wavenumber range from 1800 600 cm−1 mid region. Here, novel bioanalytical concept correlating these features with corresponding mass spectrometry datasets unravel metabolic clusters within complex plant tissues was applied. As proof...
ABSTRACT Nucleotide limitation and imbalance is a well described phenomenon in animal research but understudied the plant field. A peculiarity of pyrimidine de novo synthesis plants complex subcellular organization. Here, we studied two organellar localized enzymes pathway, with chloroplast aspartate transcarbamoylase (ATC), mitochondrial dihydroorotate dehydrogenase (DHODH). ATC knockdowns were most severely affected, exhibiting low levels nucleotides, energy state, reduced photosynthetic...