A5019161688- Plant nutrient uptake and metabolism
- Plant Parasitism and Resistance
- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Crop Yield and Soil Fertility
- Plant and animal studies
- Porphyrin Metabolism and Disorders
- Legume Nitrogen Fixing Symbiosis
- Rice Cultivation and Yield Improvement
- Plant responses to water stress
- Magnetic and Electromagnetic Effects
- Cassava research and cyanide
- Mitochondrial Function and Pathology
- Biochemical and Molecular Research
- Plant Stress Responses and Tolerance
- Folate and B Vitamins Research
- Nematode management and characterization studies
- Chromosomal and Genetic Variations
- Agronomic Practices and Intercropping Systems
- Metabolism and Genetic Disorders
- Plant Gene Expression Analysis
- Food composition and properties
- Pesticide and Herbicide Environmental Studies
- Enzyme Structure and Function
- Isotope Analysis in Ecology
University of Florida
2015-2024
Washington State University
2012
Maize (Zea mays) is a major staple crop in Africa, where its yield and the livelihood of millions are compromised by parasitic witchweed Striga. Germination Striga induced strigolactones exuded from maize roots into rhizosphere. In germplasm collection, we identified two strigolactones, zealactol zealactonoic acid, which stimulate less germination than strigolactone, zealactone. We then showed that single cytochrome P450, ZmCYP706C37, catalyzes series oxidative steps maize-strigolactone...
Cereal yields decrease when grain fill proceeds under conditions of prolonged, moderately elevated temperatures. Endosperm-endogenous processes alter both rate and duration dry weight gain, but underlying mechanisms remain unclear. Heat effects could be mediated by either abnormal, premature cessation storage compound deposition or accelerated implementation normal development. This study used controlled environments to isolate temperature as the sole environmental variable during Zea mays...
Abstract Maize (Zea mays) kernels are the largest cereal grains, and their endosperm is severely oxygen deficient during grain fill. The causes, dynamics, mechanisms of acclimation to hypoxia minimally understood. Here, we demonstrate that develops in small, growing endosperm, but not nucellus, becomes standard state, regardless diverse structural genetic perturbations modern maize (B73, popcorn, sweet corn), mutants (sweet4c, glossy6, waxy), non-domesticated wild relatives (teosintes...
Abstract Selection for yellow- and white-grain types has been central to postdomestication improvement of maize. While genetic control carotenoid biosynthesis in endosperm is attributed primarily the Yellow1 (Y1) phytoene synthase gene, less known about role dominant white factor White Cap (Wc). We show that Wc locus contains multiple, tandem copies a Carotenoid cleavage dioxygenase 1 (Ccd1) gene encodes carotenoid-degrading enzyme. A survey 111 maize inbreds landraces, together with 22...
Abiotic stresses reduce crop growth and yield in part by disrupting metabolic homeostasis triggering responses that change the metabolome. Experiments designed to understand mechanisms underlying these metabolomic have usually not used agriculturally relevant stress regimes. We therefore subjected maize plants drought, salt, or heat mimic field conditions analyzed leaf at metabolome transcriptome levels. Shared features of metabolomes included synthesis raffinose, a compatible solute...
The B vitamin thiamin is essential for central metabolism in all cellular organisms including plants. While plants synthesize de novo, organs vary widely their capacities synthesis. We use a transcriptomics approach to appraise the distribution of novo synthesis and salvage pathways among maize. identify at least six developmental contexts which metabolically active, non-photosynthetic exhibit low expression one or both branches biosynthetic pathway indicating dependence on inter-cellular...
The maize (Zea mays) ear represents one of the most striking domestication phenotypes in any crop species, with cob conferring an exceptional yield advantage over ancestral form teosinte. Remodeling grain-bearing surface required profound developmental changes. However, underlying mechanisms remain unclear and can only be partly attributed to known gene Teosinte glume architecture 1 (Tga1). Here we show that a more complete conversion involves strigolactones (SLs), these are prominent...
Abstract Riboflavin (vitamin B2) is the precursor of flavin coenzymes mononucleotide and adenine dinucleotide. In Escherichia coli other bacteria, sequential deamination reduction steps in riboflavin biosynthesis are catalyzed by RibD, a bifunctional protein with distinct pyrimidine deaminase reductase domains. Plants have two diverged RibD homologs, PyrD PyrR; PyrR proteins an extra carboxyl-terminal domain (COG3236) unknown function. Arabidopsis (Arabidopsis thaliana) (encoded At4g20960)...
The TenA protein family occurs in prokaryotes, plants and fungi; it has two subfamilies, one (TenA_C) having an active-site cysteine, the other (TenA_E) not. TenA_C proteins participate thiamin salvage by hydrolysing breakdown product amino-HMP (4-amino-5-aminomethyl-2-methylpyrimidine) to HMP (4-amino-5-hydroxymethyl-2-methylpyrimidine); function of TenA_E is unknown. Comparative analysis prokaryote plant genomes predicted that (i) a role similar to, but not identical with, (ii) also have...
Abstract Sugar supply is a key component of hypoxia tolerance and acclimation in plants. However, striking gap remains our understanding mechanisms governing sugar impacts on low-oxygen responses. Here, we used maize (Zea mays) root-tip system for precise control oxygen levels. We compared responses to (21 0.2%) the presence abundant versus limited glucose supplies (2.0 0.2%). Low-oxygen reconfigured transcriptome with deprivation enhancing speed magnitude gene induction core anaerobic...
Abstract Strigolactones are plant hormones with roles in a wide range of signaling and developmental processes. A yellow-striped maize mutant, ( inter v einal y ellow ) ivy , was determined to have low iron tissues under normal growth conditions. The gene underlying the mutation mapped identified as ZmCCD8 key enzyme biosynthesis strigolactones. Under iron-replete conditions, comparison transcriptomes wild-type plants ccd8 mutants revealed suppression several iron-regulated genes . These...
Hydroponic approaches have been a boon for root research by facilitating root-feeding studies as well secretion analysis.Results aided our understanding of root-shoot signaling, transport, hormone function and more.However, existing are often restricted to small plants seedlings prohibitive cost or availability experimental substrates.In addition this, on the hormone, strigolactone (SL) has constrained in species like maize lack specific assays quantifying responses.Here low-volume...
Abstract Selection for yellow and white grain types has been central to post-domestication improvement of maize. While genetic control carotenoid biosynthesis in endosperm is attributed primarily the Yellow1 ( Y1 ) phytoene synthase gene, less known about role dominant factor White Cap Wc ). We show that locus contains multiple, tandem copies a Carotenoid cleavage dioxygenase 1 Ccd1 gene encodes carotenoid-degrading enzyme. A survey 111 maize inbreds landraces, together with 21 teosinte...