A5074201536- Plant nutrient uptake and metabolism
- Plant tissue culture and regeneration
- Lipid metabolism and biosynthesis
- Advanced MRI Techniques and Applications
- Rice Cultivation and Yield Improvement
- Plant Molecular Biology Research
- Spectroscopy Techniques in Biomedical and Chemical Research
- Transgenic Plants and Applications
- Genetics and Plant Breeding
- Genetic Mapping and Diversity in Plants and Animals
- Biofuel production and bioconversion
- Plant responses to water stress
- Wheat and Barley Genetics and Pathology
- Seed Germination and Physiology
- Isotope Analysis in Ecology
- Lanthanide and Transition Metal Complexes
- Advanced Neuroimaging Techniques and Applications
- Leaf Properties and Growth Measurement
- Polysaccharides and Plant Cell Walls
- Electron Spin Resonance Studies
- Plant Stress Responses and Tolerance
- Agriculture, Soil, Plant Science
- Nitrogen and Sulfur Effects on Brassica
- Photosynthetic Processes and Mechanisms
- MRI in cancer diagnosis
Leibniz Institute of Plant Genetics and Crop Plant Research
2018-2024
Abstract Even though Sugars Will Eventually be Exported Transporters (SWEETs) have been found in every sequenced plant genome, a comprehensive understanding of their functionality is lacking. In this study, we focused on the SWEET family barley (Hordeum vulgare). A radiotracer assay revealed that expressing HvSWEET11b African clawed frog (Xenopus laevis) oocytes facilitated bidirectional transfer not only just sucrose and glucose, but also cytokinin. Barley plants harboring loss-of-function...
Abstract Adventitious root (AR) formation is one of the most important developmental processes in vegetative propagation. Although genotypic differences rose rooting ability are well known, causal factors not understood. The two contrasting genotypes, ‘Herzogin Friederike’ and ‘Mariatheresia’, was compared following a multiscale approach. Using magnetic resonance imaging, we non-invasively monitored inner structure stem cuttings during initiation progression AR for first time. Spatially...
Abstract Maize (Zea mays) kernels are the largest cereal grains, and their endosperm is severely oxygen deficient during grain fill. The causes, dynamics, mechanisms of acclimation to hypoxia minimally understood. Here, we demonstrate that develops in small, growing endosperm, but not nucellus, becomes standard state, regardless diverse structural genetic perturbations modern maize (B73, popcorn, sweet corn), mutants (sweet4c, glossy6, waxy), non-domesticated wild relatives (teosintes...
Protein deficiency is recognized among the major global health issues with an underestimation of its importance. Genetic biofortification a cost-effective and sustainable strategy to overcome protein malnutrition. This study was designed focus on protein-dense grains wheat (Triticum aestivum L.) identify genes governing grain content (GPC) that improve end-use quality in turn human health. Genome-wide association applied using 90k iSELECT Infinium 35k Affymetrix arrays GPC quantified by...
Sucrose (Suc) is the major transport sugar in plants and plays a primary role as an energy source signal adaptive stress responses. An ability to quantify Suc over time space would serve advance our understanding of these important processes. Current technologies used for mapping are unable quantitatively visualize its distribution within tissues. Here, we present infrared-based microspectroscopic method that allows quantitative visualization at microscopic level resolution (∼12 µm). This...
Abstract Cereal grains contribute substantially to the human diet. The maternal plant provides carbohydrate and nitrogen sources deposited in endosperm, but basis for their spatial allocation during grain filling process is obscure. Here, vacuolar processing enzymes have been shown both mediate programmed cell death (PCD) tissues of a barley influence delivery assimilate endosperm. proposed centrality PCD has implications cereal crop improvement.
Brassicaceae seeds consist of three genetically distinct structures: the embryo, endosperm and seed coat, all which are involved in assimilate allocation during development. The complexity their metabolic interrelations remains unresolved to date. In present study, we apply state-of-the-art imaging analytical approaches assess environment Brassica napus embryo. Nuclear magnetic resonance (MRI) provided volumetric data on living embryo endosperm, revealing how envelops determining endosperm's...
Summary The initial free expansion of the embryo within a seed is at some point inhibited by its contact with testa, resulting in formation folds and borders. Although less obvious, mechanical forces appear to trigger accelerate maturation. However, mechanistic basis for this effect remains unclear. Manipulation constraints affecting either vivo or vitro growth oilseed rape embryos was combined analytical approaches, including magnetic resonance imaging computer graphic reconstruction,...
Studying dynamic processes in living organisms with MRI is one of the most promising research areas. The use paramagnetic compounds as contrast agents (CA), has proven key to such studies, but so far, lack appropriate techniques limits application CA-technologies experimental plant biology. presented proof-of-principle aims support method and knowledge transfer from medical science.In this study, we designed tested a new approach for Dynamic Contrast Enhanced Magnetic Resonance Imaging...
Magnetic resonance imaging (MRI) is a versatile technique in the biomedical field, but its application to study of plant metabolism vivo remains challenging because magnetic susceptibility problems. In this study, we report establishment chemical exchange saturation transfer (CEST) for MRI. This method enables noninvasive access sugars and amino acids complex sink organs (seeds, fruits, taproots, tubers) major crops (maize, barley, pea, potato, sugar beet, sugarcane). Because high signal...
Evaluation of relevant seed traits is an essential part most plant breeding and biotechnology programs. There need for non-destructive, three-dimensional assessment the morphometry, composition, internal features seeds. Here, we introduced a novel tool, MRI-Seed-Wizard, which integrates deep learning algorithms with non-invasive magnetic resonance imaging (MRI) its use in new domain - MRI. The tool enabled vivo quantification 23 grain traits, including volumetric parameters inner structure....