ABSTRACT Background Gliomas currently have a poor prognosis and limited therapy options. Betulinic acid (BA) has demonstrated antitumor activity in various cancers. This study is aimed at clarifying the underlying mechanisms by which BA inhibits gliomas. Methods We assessed how affected migration, apoptosis, invasion, proliferation, viability of U251 glioma cells. The genes that were differentially expressed after treatment identified via RNA sequencing. Utilizing Gene Ontology Kyoto...

Gliomas are the most common form of primary brain tumor in adult central nervous system. Altered expression and prognostic value transmembrane protein 97 (TMEM97) has been recently reported different types human tumors. However, association TMEM97and glioma is poorly defined. Here, we that TMEM97 was significantly increased tissues compared to non-tumorous tissues. Furthermore, levels were progressively with increasing histologic grade glioma. Higher level correlated shorter survival time...

Increasing evidence has revealed that nicotinamide N-methyltransferase (NNMT) is a key factor influencing the prognosis of tumors. The present study aimed to investigate role NNMT in glioma and elucidate associated functional mechanisms.Clinical samples were analyzed by immunohistochemical staining Western blotting evaluate expression normal brain tissues. correlation between was using Cancer Genome Atlas (TCGA) database. Additionally, knocked down two types cells, U87 U251, invasive ability...

Glioblastoma is a highly aggressive brain tumor characterized by high recurrence and poor prognosis. Vitexin has shown activities against esophageal, liver, lung, colorectal, ovarian cancers; however, there little knowledge on the activity of vitexin glioblastoma. This study was therefore designed with aims to examine effects proliferation, invasion, apoptosis human U251 glioblastoma cells explore underlying molecular mechanisms using mRNA sequencing docking. found inhibit cell colony...

Prolactinoma (prolactin-secreting pituitary adenoma) is one of the most common estrogen-related functional tumors. As an agonist dopamine D2 receptor, bromocriptine used widely to inhibit prolactinoma progression. On other hand, it not always effective in clinical application. Although a receptor deficiency contributes impaired efficiency therapy some extent, unknown whether there underlying mechanisms leading resistance treatment. That main point addressed this project.Human samples were...

We have found that in the presence of hydroxylamine, heme prosthetic group protein adsorbed at mercury electrode surface reacts with ion produced by electrochemical oxidation and is quantitatively converted into protoporphyrin IX using single-sweep polarography. As a result, small redox peak P0 about −0.46 V (vs SCE) disappears large new reduction P −0.89 comes out pH 9.6 NaHCO3−Na2CO3 solution. Peak extremely sensitive to concentration. On basis P, unique method for assays constructed. For...

Objective To investigate the learning curve under different surgical complexity in endoscopic transsphenoidal approach for pituitary adenoma. Methods 273 patients undergoing surgery adenoma were collected retrospectively and divided into three groups chronologically (early, middle, late periods). Surgical was differentiated based on Knosp classification (Knsop grade 0–2 vs. 3–4), tumor maximum diameter (MD) (macroadenomas giant adenomas), history of previous (first operation reoperation)....

Among the common malignancies, colorectal cancer (CRC) is often resistant to chemotherapy because of drug resistance and severe toxicity. Currently, aspirin one most promising CRC chemopreventive drugs, both for primary prevention reducing chance recurrence metastasis following radical surgery in patients with early-stage CRC. Oleanolic acid a potential antineoplastic that has an antagonistic effect on many kinds tumors. Network pharmacology, molecular docking, vitro experiments were...

Oleanolic acid is a promising drug for treating gliomas, but its underlying mechanism unclear. This study aimed to determine the potential effect of oleanolic on glioma and mechanism. Firstly, effects proliferation, invasion, apoptosis U251 cells were detected by in vitro experiments such as MTT assay, cell cloning, flow cytometry. The transcriptome data treated with untreated sequenced mRNA, then differentially expressed genes analyzed gene ontology (GO), genomic encyclopedia (KEGG) pathway...

A disintegrin and metalloproteinase domain‐containing protein 10 (ADAM10) is a known to modulate the progression of several types tumor. However, role played by ADAM10 in pituitary adenomas currently unknown, what factors orchestrate activation this kind tumor also unclear. Here, we found that SRC kinase an ADAM10‐interacting partner activity required for interaction. As new positive regulator promoting shedding ADAM10, could compete with calmodulin 1 (CALM1) binding mutually exclusive...

The human glutamate receptor delta 2 gene (GRID2) shares 90% homology with the orthologous mouse gene. Grid2 is involved functions of cerebellum and spontaneous mutation leads to a spinocerebellar ataxia-like phenotype. To investigate whether such mutations occur in humans, we screened for coding sequence GRID2 24 patients familial or sporadic ataxia 52 normal controls. We detected no point insertion/deletion 16 exons GRID2. However, polymorphic 4 nucleotide deletion (IVS5-121_-118 GAGT) two...

OBJECTIVE To study the therapeutic efficacy and mechanism of oxime drug, pralidoxime chloride (PAM-Cl), on acute dichlorvos (DDV) poisoning. METHODS The toxic signs survival rate were recorded ChE activity in blood was determined treatment group with PAM-Cl non-treatment after DDV given to rats mice by gastrogavage; reactivation DDV-inhibited observed patients RESULTS (1) alleviated delayed as well higher found compared group. (2) After exposed DDV, activities whole different time within 24...

Improving quality of areca nut is a critical problem for manufacturers. A steam experimental explosion device has been developed. An processing technology was optimized by using the orthogonal experiment method. In which, parameters including pressure, time and temperature, were taken three factors. Each factor selected levels nine groups experiments carried out. The volume expansion rate sample as evaluation standard; best treatment scheme obtained through optimization. results showed that...