Drainage of parenchymal waste through the lymphatic system maintains brain homeostasis. Age-related changes glymphatic-lymphatic clearance lead to accumulation beta-amyloid (Aβ) in dementia models. In this study, focused ultrasound treatment combination with microbubbles (FUS-MB) improved Aβ drainage early model mice, 5XFAD. FUS-MB enhanced solute from brain, but not plaques, cerebrospinal fluid (CSF) space and then deep cervical lymph node (dCLN). dCLN ligation exaggerated memory impairment...

Abstract Background Dynamically altered microglia play an important role in the progression of Alzheimer’s disease (AD). Here, we found a close association metabolic reconfiguration with increased hippocampal glucose uptake on [ 18 F]fluorodeoxyglucose (FDG) PET. Methods We used AD animal model, 5xFAD, to analyze metabolism using both FDG PET and ex vivo test. Cells hippocampus were isolated perform single-cell RNA-sequencing (scRNA-seq). The molecular features cells associated analyzed at...

Although fish gelatin has become a research hotspot in recent years, researchers and manufacturers are still looking for high-quality sources of galatin to meet the commercial demand safer gelatin.became This study aimed evaluate impact seasonal variation farming systems on properties extracted from Nile tilapia scales. Gelatin farmed had lowest impurities, higher clarity as well desirable color characteristics (L* = 65.95 a* −0.33). The protein fat composition Wild (91.00 ± 0.00c) 1.94...

Mycotoxins, especially aflatoxin B1 (AFB1) and fumonisin (FMB1), are common contaminants in cereal-based foods. Instances of contamination predicted to increase due the current challenges induced by climate change. Despite health benefits whole grains, presence mycotoxins bran remains a concern. Nonetheless, previous research indicates that wheat can adsorb mutagens. Therefore, this study investigated capacity maize, wheat, oat brans AFB1 FMB1 under varying

Alveolar bone resorption caused by trauma or periodontal diseases has represented a challenge for both dental clinicians and researchers. In this study, we evaluate the osteogenic potential of human gingival fibroblasts (HGFs) through direct transdifferentiation from HGFs to functional osteoblasts via epigenetic modification signaling with morphogenetic protein 2 (BMP2) in vitro vivo. HGF treatment 5-aza-2′-deoxycytidine (5-aza-dC) induced demethylation hypermethylated CpG islands lineage...

This study was conducted to investigate the influence of dietary rapeseed meal (RSM) on growth performance, blood profiles, nutrient digestibility and economic benefit growing-finishing pigs. A total 120 growing pigs ([Yorkshire×Landrace] ×Duroc) with an initial body weight (BW) 29.94±0.06 kg were used in this experiment. Pigs randomly allotted into 1 5 treatments a randomized complete block design 6 replicates 4 per pen. Treatments divided by RSM supplementation levels (0%, 3%, 6%, 9%, or...

TRPM4 channels are Ca2+-activated nonselective cation which deeply involved in physiological and pathological conditions. However, their trafficking mechanism binding partners still elusive.We have found the 14-3-3γ as a partner for TRPM4b using its N-terminal fragment from yeast-two hybrid screening. Ser88 at N-terminus of is critical by showing GST pull-down co-immunoprecipitation. Heterologous overexpression HEK293T cells increased expression on plasma membrane was measured whole-cell...

When students solve problems, their proficiency in a particular subject may influence how well they perform similar, but different area of study. For example, studies have shown that science ability an effect on the mastery mathematics skills, which turn affect examinees respond to items. From this view, it becomes natural examine relationship performance study attributes related subject. To such influence, proposes covariate extension deterministic input noisy “and” gate (DINA) model by...

The objective of this study was to assess consumer behaviour towards tilapia and products provide information linking production with consumption patterns preferences as well predict factors that influence preference, purchase behaviour, willingness patronize fillets using classification regression trees. A total 960 responses were obtained convenient sampling. findings survey indicate is eaten mainly because its taste. Regarding the various cooked options available in Ghana, 58.5% preferred...

Feruloylated arabinoxylan (AX) is a potential health-promoting fiber ingredient that can enhance nutritional properties of bread but also known to affect dough rheology. To determine the role feruloylation and hydrolysis wheat bran AX on quality microstructure, hydrolyzed unhydrolyzed fractions with low high ferulic acid content were produced, their chemical composition evaluated. These then incorporated into dough, farinograph measurements, large small deformation measurements...

Four artificial sweeteners, i.e., sodium saccharin, aspartame, acesulfame-K and sucralose, are permitted for use in Korea, recent regulatory changes have expanded the number of food categories which they may be used. sweeteners were determined simultaneously more than 900 items from 30 that commercially available Korean markets, including both domestic imported products, using high-performance liquid chromatography evaporative light-scattering detection (ELSD). A new procedure 75% acetone to...

Abstract Aquaculture is a key component of Ghana's food system strategies, promoting sustainable animal protein production. complements traditional marine capture fisheries to close the gap between demand and supply. However, about one third fish produced globally have been reported go waste yearly. Among various cultured fish, Nile tilapia Oreochromis niloticus predominant species. This study aimed estimate extent wastage by consumers in Ghana. A web‐based cross‐sectional consumer survey...

Abstract Drainage of parenchymal waste through the lymphatic system maintains brain homeostasis. Age-related changes glymphatic-lymphatic clearance lead to accumulation beta-amyloid (Aβ) in dementia models. In this study, focused ultrasound treatment combination with microbubbles (FUS-MB) improved Aβ drainage early model mice, 5XFAD. FUS-MB enhanced solute from brain, but not plaques, cerebrospinal fluid (CSF) space and then deep cervical lymph node (dCLN). dCLN ligation exaggerated memory...

Background: Profiles of amino acid metabolites (AAMs) have been linked to obesity and energy homeostasis. We investigated whether baseline obesity-related AAMs were associated with weight status in the early postoperative period after sleeve gastrectomy. Methods: In this prospective, single-arm, longitudinal study, 27 bariatric patients underwent Twenty comprehensively quantified prior surgery, slow loss was defined as lowest 40% percentage excess (%EWL) at three six months postoperatively....

To understand the transcriptional regulation of human insulin-like growth factor II (IGF-II) gene, we examined effects okadaic acid, a potent in vitro inhibitor protein phosphatases, on activation IGF-II gene expression. Treatment A-549 lung adenocarcinoma cells with acid increased expression mRNAs. Since 4.8-kb mRNA is transcribed under control P4 promoter, promoter element responsible for acid-mediated activation. Transfection promoter-chloramphenicol acetyltransferase constructs...

Heterocyclic amines (HCAs) are potent mutagens generated by the high temperatures of cooking process. The purpose this study was to develop and validate analytical methods for HCAs determination using high-performance liquid chromatography-tandem mass spectrometry in seven food matrices: corn oil, milk, 20% ethanol, pork, flat fish, sea mustard (Undaria pinnatifida), radish. Six isotopically labelled internal standards were used quantitation, Chem Elut Oasis hydrphilic-liphophilic balance...

Heterocyclic amines (HCAs) are contaminants in proteinaceous foods produced by cooking at high temperatures. This study was the first assessment of exposure to HCAs using Korean total diet study. Twelve were analysed 1,232 pooled samples six isotope-labelled internal standards and HPLC-MS/MS. The daily intake population estimated based on concentration individual food consumption data from National Health Nutrition Examination Survey. Among HCAs, β-carbolines, such as harman norharman,...