A5046141864- Retinal Development and Disorders
- Retinal Diseases and Treatments
- DNA Repair Mechanisms
- Photoreceptor and optogenetics research
- CRISPR and Genetic Engineering
- Cancer-related Molecular Pathways
- Cancer Research and Treatments
- PARP inhibition in cancer therapy
- Homelessness and Social Issues
- Gastric Cancer Management and Outcomes
- Polyomavirus and related diseases
- bioluminescence and chemiluminescence research
- Retinoids in leukemia and cellular processes
- T-cell and B-cell Immunology
- Multiple and Secondary Primary Cancers
- Photochromic and Fluorescence Chemistry
- Healthcare professionals’ stress and burnout
- Mental Health and Patient Involvement
- Hepatitis C virus research
- Virus-based gene therapy research
- Cell death mechanisms and regulation
- Papaya Research and Applications
- Galectins and Cancer Biology
- Animal Genetics and Reproduction
- Endoplasmic Reticulum Stress and Disease
National Institutes of Health
2010-2023
National Eye Institute
2020-2022
Protein Express (United States)
2015
National Cancer Institute
2010-2014
Mario Negri Institute for Pharmacological Research
2002-2014
Istituti di Ricovero e Cura a Carattere Scientifico
2014
University of Milan
2014
Fondazione Filarete
2014
Society for Women's Health Research
2013
Homologous recombination (HR) is initiated by DNA end resection, a process in which stretches of single-strand (ssDNA) are generated and used for homology search. Factors implicated resection include nucleases MRE11, EXO1, DNA2, ends into 3′ ssDNA overhangs; helicases such as BLM, unwind DNA; other proteins BRCA1 CtIP whose functions remain unclear. CDK-mediated phosphorylation on T847 required to promote whereas CDK-dependent CtIP-S327 interaction with BRCA1. Here, we provide evidence that...
Abstract Purpose: The PRL-3 phosphatase has been found expressed at higher levels in metastasis than primary tumors of patients with colorectal cancer. In the present study, we evaluated expression ovarian cancer tissue and its role cell growth. Experimental Design: was 84 tumor samples. knocked down using specific small interfering RNAs to determine growth vitro. Results: cancers, correlates disease progression, being advanced (stage III) early I) tumors. situ measurements showed that it...
DNA double-strand breaks (DSBs) are byproducts of normal cellular metabolism and obligate intermediates in antigen receptor diversification reactions. These lesions potentially dangerous because they can lead to deletion genetic material or chromosome translocation. The chromatin-binding protein 53BP1 the histone variant H2AX required for efficient class switch (CSR) V(D)J recombination part protect ends from resection thereby favor nonhomologous end joining (NHEJ). Here, we examine...
The cytoprotective effects of pigment epithelium-derived factor (PEDF) require interactions between an as a yet undefined region with distinct ectodomain on the PEDF receptor (PEDF-R). Here we characterized area in that interacts PEDF-R to promote photoreceptor survival. Molecular docking studies suggested ligand binding site neurotrophic (44-mer, positions 78–121). Binding assays demonstrated bound 44-mer peptide. Moreover, peptide P1 from had affinity for and shorter fragment within it,...
p53 influences genomic stability, apoptosis, autophagy, response to stress, and DNA damage. New p53-target genes could elucidate mechanisms through which controls cell integrity damage.DRAGO (drug-activated gene overexpressed, KIAA0247) was characterized by bioinformatics methods as well real-time polymerase chain reaction, chromatin immunoprecipitation luciferase assays, time-lapse microscopy, viability assays. Transgenic mice (94 p53(-/-) 107 p53(+/-) on a C57BL/6J background) were used...
Purpose: To examine the contribution of pigment epithelium-derived factor receptor (PEDF-R) to phagocytosis process. Previously, we identified PEDF-R, protein encoded by PNPLA2 gene, as a phospholipase A2 in retinal epithelium (RPE). During phagocytosis, RPE cells ingest abundant phospholipids and form photoreceptor outer segment (POS) tips, which are then hydrolyzed. The role PEDF-R is not known. Methods: Mice was conditionally knocked out (cKO) were generated. Mouse RPE/choroid explants...
Autoimmune diseases (ADs) impose substantial health and financial burdens in the United States many parts of world. Women are disproportionately affected by these disorders, which often contribute to lifelong disabilities. While number patients with some ADs appears be rising, complexities conducting epidemiological studies prevent a thorough understanding prevalence incidence various conditions. Research on environmental influences illnesses is limited, although they generally hypothesized...
DNA double-strand breaks (DSBs) can be repaired locally or recombined to produce chromosome rearrangements.
Abstract Purpose To examine the contribution of PEDF-R to phagocytosis process. Previously, we identified PEDF-R, protein encoded by PNPLA2 gene, as a phospholipase A2 in retinal pigment epithelium (RPE). During phagocytosis, RPE cells ingest abundant phospholipids and form photoreceptor outer segment (POS) tips, which are then hydrolyzed. The role is not known. Methods Mice was conditionally knocked out were generated (cKO). Mouse RPE/choroid explants cultured. Human ARPE-19 transfected...
Precise determination of transgene zygosity is essential for use transgenic mice in research. Because integration loci transgenes are usually unknown due to their random insertion, assessment remains a challenge. Current genotyping methods (progeny testing, qPCR, and NGS-computational biology analysis) time consuming, prone error or technically challenging. Here, we developed novel method determine requiring no knowledge insertion loci. This applies allele-specific restriction enzyme...