A5085313408- Radiopharmaceutical Chemistry and Applications
- Peptidase Inhibition and Analysis
- Medical Imaging Techniques and Applications
- Monoclonal and Polyclonal Antibodies Research
- Prostate Cancer Treatment and Research
- Antibiotic Resistance in Bacteria
- Antifungal resistance and susceptibility
- Medical Imaging and Pathology Studies
- Bacterial Infections and Vaccines
- Neuroendocrine Tumor Research Advances
- Mycotoxins in Agriculture and Food
- Trace Elements in Health
- Bacterial Genetics and Biotechnology
- Cell Adhesion Molecules Research
- HER2/EGFR in Cancer Research
- Lung Cancer Research Studies
- Nanoparticle-Based Drug Delivery
- Sarcoma Diagnosis and Treatment
- Caveolin-1 and cellular processes
- Nanowire Synthesis and Applications
- Effects of Environmental Stressors on Livestock
- RNA Interference and Gene Delivery
- MRI in cancer diagnosis
- Heavy Metal Exposure and Toxicity
- Bacterial biofilms and quorum sensing
Institute of Molecular and Translational Medicine
2014-2025
Palacký University Olomouc
2015-2025
Advanced Technology Group (Czechia)
2025
Charles University
2008-2014
Pseudomonas aeruginosa is an increasingly prevalent opportunistic pathogen that causes a variety of life-threatening nosocomial infections. Novel strategies for the development new antibacterial treatments as well diagnostic tools are needed. One novel detection infection could be utilization siderophores. Siderophores low-molecular-weight chelators produced by microbes to scavenge essential iron. Replacing iron in siderophores suitable radiometals, such Ga-68 positron emission tomography...
With the increase of especially hospital-acquired infections, timely and accurate diagnosis bacterial infections is crucial for effective patient care. Molecular imaging has potential specific sensitive detection infections. Siderophores are iron-specific chelators recognized by transporters, representing one few fundamental differences between mammalian cells. Replacing iron gallium-68 without loss bioactivity possible allowing molecular positron emission tomography (PET). Here, we report...
Currently, targeted alpha therapy is one of the most investigated topics in radiopharmaceutical cancer management. Especially, emitter
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Abstract The present study focuses on the development of a sensitive and specific approach for visualization sentinel lymph nodes draining tumor using ultrabright 200‐nm fluorescent nanodiamonds (FNDs) equipped with designed targeting surface architecture. FNDs narrow size distribution are isolated by differential centrifugation, colloidally stabilized alkyne‐functionalized poly(glycerol) modified polyvalent array mannose (FND‐p‐Man). In vitro experiments demonstrate an outstanding increase...
Abstract Background Siderophores are small iron-binding molecules produced by microorganisms to facilitate iron acquisition from the environment. Radiolabelled siderophores offer a promising solution for infection imaging, as they can specifically target pathophysiological mechanisms of pathogens. Gallium-68 replace in siderophores, enabling molecular imaging with positron emission tomography (PET). Stereospecific interactions play crucial role recognition receptors, transporters, and...
Some [68Ga]siderophores show promise in specific and sensitive imaging of infection. Here, we compare the vitro vivo behaviour selected Ga-68 Zr-89 labelled siderophores. Radiolabelling was performed HEPES or sodium acetate buffer systems. Radiochemical purity siderophores determined using chromatography. Partition coefficients, stability protein binding affinities were determined. Ex biodistribution animal studied mice. Certain differences among observed labelling efficiency. Protein tests...
Bacteria from the Burkholderia cepacia complex are generally considered to be non-pathogenic healthy population. However, some of these species may cause serious nosocomial infections in immunocompromised patients; as such, it is essential diagnose rapidly so that adequate treatment can initiated. We report here use a radiolabeled siderophore, ornibactin (ORNB), for positron emission tomography imaging. successfully ORNB with gallium-68 high radiochemical purity and proved resulting has...
Acinetobacter baumannii (AB) is an opportunistic pathogen with growing clinical relevance due to its increasing level of antimicrobial resistance in the last few decades. In event AB hospital outbreak, fast detection and localization crucial, prevent further spread. However, contemporary diagnostic tools do not always meet requirements for rapid accurate diagnosis. For this reason, we report here possibility using gallium-68 labeled siderophores, bacterial iron chelators, positron emission...
Overexpression of human epidermal growth factor receptor type 2 (HER2) occurs in multiple carcinomas. For example, up to 20% breast cancer cases are classified as HER2 positive (HER2+). Treatment this condition typically involves immunotherapy using monoclonal antibodies, such trastuzumab or pertuzumab. The precise targeting antibodies HER2+ tumour lesions can be used well radioimmunotherapy deliver medical radionuclides exactly the afflicted area and therefore minimize radiation exposure...
Positron emission tomography (PET) as well optical imaging (OI) with peptide receptor targeting probes have proven their value for oncological applications but also show restrictions depending on the clinical field of interest. Therefore, combination both methods, particularly in a single molecule, could improve versatility routine. This proof principle study aims to that chelator, Fusarinine C (FSC), can be utilized scaffold novel dimeric dual-modality agents. Two vectors (a minigastrin...
Aspergillus fumigatus is a ubiquitous saprophytic airborne fungus responsible for more than one million deaths every year. The siderophores of A. represent important virulence factors that contribute to the microbiome-metabolome dialog in host. From diagnostic point view, monitoring secondary metabolites urine host promising due non-invasiveness, rapidity, sensitivity and potential standardization. Using model experimental aspergillosis immunocompromised Lewis rats, fungal ferricrocin (FC)...
Aspergillus fumigatus produces the siderophore triacetylfusarinine C (TAFC) for iron acquisition which is essential its virulence. Therefore, TAFC a specific marker invasive aspergillosis. We have shown previously that positron emission tomography (PET) imaging with [68Ga]TAFC exhibited excellent targeting properties in an A. rat infection model. In this study, we aimed to prepare analogs modifying fusarinine (FSC) by acylation different carbon chain lengths as well charged substituents and...
(68)Ga-triacetylfusarinine C (TAFC) and (68)Ga-ferrioxamine E (FOXE) show great potential to be used as highly sensitive selective tracers for Aspergillus infection imaging. Here we report on a comparison of the ex vivo biodistribution small animal imaging (68)Ga-TAFC (68)Ga-FOXE versus (68)Ga-colloid (68)Ga-citrate unspecific control in mice.The radiochemical purity tested (68)Ga labelled was determined by RP-HPLC or ITLC-SG. Ex studied normal DBA/2 mice 30 min 90 p.i. Static dynamic were...
Lasioglossins are a group of peptides with identified antimicrobial activity. The inhibitory effects two synthetic lasioglossin derivatives, LLIII and D-isomeric variant LLIII-D, on morphological changes in Candida albicans vitro the effect local administration during experimental murine candidiasis were investigated. C. blastoconidia grown presence or LLIII-D at concentrations 11.5 μM 21 μM, respectively, for 1, 2 3 days their viability determined by flow cytometry using eosin Y staining....
In this study, we have compared four 68Ga-labeled peptides (three Arg-Gly-Asp (RGD) and substance-P) with two 18F-tracers clinically approved for tumor imaging. We studied in vitro vivo characteristics of selected radiolabeled tracers a glioblastoma multiforme model. The part the study was mainly focused on evaluation radiotracers stability under various conditions. also determined 68Ga-radiotracers by analysis murine urine collected at time points after injection. behavior tested...
As angiogenesis plays a key role in tumor growth and metastasis, the angiogenic process has attracted scientific interest as target for diagnostic therapeutic agents. Factors influencing include vascular endothelial factor (VEGF) family two associated receptor types (VEGFR-1 VEGFR-2). VEGFR-1/-2 detection quantification cancer lesions are essential management. result of advantageous pharmacokinetics image contrast, peptides radiolabeled with PET emitters have become interesting tools...
Nanoparticles (NPs) represent an emerging platform for diagnosis and treatment of various diseases such as cancer, where they can take advantage enhanced permeability retention (EPR) effect solid tumor accumulation. To improve their colloidal stability, prolong blood circulation time avoid premature entrapment into reticuloendothelial system, coating with hydrophilic biocompatible polymers is often essential. Most studies, however, employ just one type polymer. The main purpose this study to...
Monoclonal antibodies are used in the therapy of various diseases. Thanks to their high specific uptake target tissues, these can be utilized targeted radioimmunotherapy as carriers radioisotopes tumors. However, important characteristics such binding and stability organism may affected by structural parameters. This study has focused on potential influence selected chelators radiochemical quality vitro receptor capacity two modified monoclonal antibodies—cetuximab panitumumab, both ligands...