A5102959056- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Marine Sponges and Natural Products
- Microbial Natural Products and Biosynthesis
- Natural product bioactivities and synthesis
- Bioactive Compounds and Antitumor Agents
- Phytochemical compounds biological activities
- Crystallography and molecular interactions
- Synthesis and Biological Activity
- Essential Oils and Antimicrobial Activity
- Toxin Mechanisms and Immunotoxins
- Cancer therapeutics and mechanisms
- Marine Biology and Environmental Chemistry
- Phytochemistry and Bioactive Compounds
- Synthetic Organic Chemistry Methods
- Phytochemistry and Biological Activities
- Natural Antidiabetic Agents Studies
- Natural Compounds in Disease Treatment
- ATP Synthase and ATPases Research
- Fungal Biology and Applications
- Bioactive natural compounds
- Marine Ecology and Invasive Species
- Phytochemistry and Bioactivity Studies
- Marine Toxins and Detection Methods
- Metabolomics and Mass Spectrometry Studies
Heinrich Heine University Düsseldorf
2016-2025
Institute of Environmental Biology and Biotechnology
2019
Annamalai University
2017
Hochschule Düsseldorf University of Applied Sciences
2017
University of Würzburg
1993-1999
Sponges of the Aplysinidae family contain large amounts bacteria that are embedded within sponge tissue matrix. In order to determine stability and specificity Aplysina–microbe association, sponges were maintained in recirculating seawater aquariums for 11 days. One aquarium was left untreated, a second one contained 0.45 μm filtered (starvation conditions) third plus antibiotics (antibiotics exposure). Changes microbial community monitored using group-specific, 16S rRNA targeted...
The Mediterranean sponges Aplysina aerophoba and A. cavernicola accumulate brominated isoxazoline alkaloids including aplysinamisin-1 (1), aerophobin-2 (2), isofistularin-3 (3) or aerothionin (4) at concentrations up to 10% of their respective dry weights. In laboratory feeding experiments employing the polyphagous fish Blennius sphinx crude extracts both were incorporated into artificial food physiological (based on volume) offered B. in choice against untreated control food. addition...
Abstract Enniatins are mycotoxins which have important impact on human health, e.g . as contaminants of cereals, but also discussed possible anticancer agents. We investigated toxic effects enniatins A1, B and B1 isolated from Fusarium tricinctum different cancer cell lines. The showed moderate activity in HepG2 C6 cells (EC 50 ‐values approximately 10–25 μM), were highly H4IIE 1–2.5 μM). In cells, all increased caspase 3/7 nuclear fragmentation markers for apoptotic death. Enniatin enniatin...
Four tetrahydroxanthone dimers (1-4) and four biogenetically related monomers (5-8), including the new derivatives 4-6, were isolated from endophyte Phomopsis longicolla. The absolute configurations of 2-4 established for first time by TDDFT electronic circular dichroism calculations, that phomoxanthone A (1) was revised X-ray crystallography. Phomoxanthone showed strongest pro-apoptotic activity when tested against a panel human cancer cell lines, cisplatin-resistant cells, whereas it up to...
Purpose.Several studies have shown that natural compounds like resveratrol or ellagic acid anticancer and antioxidant properties can stimulate apoptosis in many cancer cell lines.The aim of this study was to elucidate if acid, respectively, could improve the efficacy cisplatin ovarian cancer.Methods.As a cellular resistance model, epithelial line A2780 its cisplatin-resistant subclone A2780CisR were used.A2780CisR obtained by intermittent treatment with for 26 weekly cycles showed 4-6-fold...
A new epidithiodiketopiperazine (ETP), pretrichodermamide G (1), along with three known (epi)dithiodiketopiparazines (2-4) were isolated from cultures of Trichoderma harzianum and Epicoccum nigrum, endophytic fungi associated medicinal plants Zingiber officinale Salix sp., respectively. The structure the compound (1) was established on basis spectroscopic data, including 1D/2D NMR HRESIMS. compounds investigated for their antifungal, antibacterial cytotoxic potential against a panel...
The supply problem with regard to drug development and sustainable production lies in the limited amounts of biomass most marine invertebrates available from wild stocks. Thus, pharmacologically active natural products can only be isolated minute yields. Total synthesis has been successfully established but is many cases economically not feasible due complexity molecular structures low To solve pressing issue discovery, other strategies appear more promising. One these mariculture which...
Abstract With an increasing cancer rate worldwide, there is urgent quest for the improvement of anticancer drugs. One main problems present chemotherapy in treatment tumor patients toxicity Most existent drugs, unfortunately, attack also proliferating normal cells. In recent years, traditional Chinese herbal remedies have gradually gained considerable attention as a new source Although their healing mechanisms are still largely unknown, some drugs been used to help fight disease at reduced...
We characterized the brominated alkaloid Isofistularin-3 (Iso-3), from marine sponge Aplysina aerophoba, as a new DNA methyltransferase (DNMT)1 inhibitor. Docking analysis confirmed our in vitro DNMT inhibition data and revealed binding of Iso-3 within site DNMT1. Subsequent increased expression tumor suppressor gene aryl hydrocarbon receptor (AHR) could be correlated to decreased methylation CpG sites essential Sp1 regulatory region its promoter. induced growth arrest cancer cells G0/G1...
Polybrominated diphenyl ethers (PBDEs) are natural products with potent antimicrobial and antineoplastic activity. We have previously shown that the polybrominated ether bromoxib (4,5,6-tribromo-2-(2′,4′-dibromophenoxy) phenol), isolated from marine sponge Dysidea species, exhibits a strong cytotoxic potential in leukemia lymphoma cells by targeting mitochondrial metabolism. Here, using mass spectrometric thermal proteome profiling (TPP) approach, we observed induces rapid reduction levels...
Crude extracts from different Aglaia species are used as anti-inflammatory remedies in the traditional medicine of several countries Southeast Asia. Because NF-kappaB transcription factors represent key regulators genes involved immune and inflammatory responses, we supposed that effects mediated by inhibition activity. Purified compounds species, namely 1H-cyclopenta[b]benzofuran lignans rocaglamide type well one aglain congener were tested for their ability to inhibit We show a group...
Abstract: A suppressive subtractive hybridization technique was used to identify genes, which were induced during the early phases of interaction between dodder (Cuscuta reflexa), a phanerogamic parasite, and its incompatible host plant tomato. One identified genes encodes tomato xyloglucan endotransglycosylase/hydrolase (XTH) ‐ an enzyme involved in cell wall elongation restructuring. The corresponding LeXTH1 mRNA accumulated 6 h after attachment parasite. In contrast, wounding did not...
Sponges (phylum Porifera) live in a symbiotic relationship with microorganisms, primarily bacteria. Until now, molecular proof for the capacity of sponges to recognize fungi surrounding aqueous milieu has not been available. Here we demonstrate, demosponge Suberites domuncula (Porifera, Demospongiae, Hadromerida), cell surface receptor that recognizes (1→3)‐β‐ d ‐glucans, e.g. curdlan or laminarin. This receptor, ‐glucan‐binding protein, was identified and its cDNA analysed. The gene coding...
Aglaia (family Meliaceae) plants are used in traditional medicine (e.g., Vietnam) for the treatment of inflammatory skin diseases and allergic disorders such as asthma. Inflammatory arise from inappropriate activation immune system, leading to abnormal expression genes encoding cytokines tissue-destructive enzymes. The active compounds isolated these derivatives rocaglamide. In this study we show that rocaglamides potent immunosuppressive phytochemicals suppress IFN-gamma, TNF-alpha, IL-2,...
ABSTRACT. Euglena gracilis is able to synthesize adenosine triphosphate under anaerobic conditions through a malonyl‐independent fatty acid synthesis leading wax ester fermentation. Mitochondrial uses acetyl‐CoA and propionyl‐CoA as C2‐ C3‐donors for de novo of even‐ odd‐numbered acids, respectively. Euglena' s fermentation has only been described in the E. strain 1224‐5/25 Z. Here we investigate eight strains isolated 1932–1958 from different localities Europe two bleached substrains...
A novel macrolide, callyspongiolide, whose structure was determined by comprehensive analysis of the NMR and HRMS spectra, isolated from marine sponge Callyspongia sp. collected in Indonesia. The compound features a carbamate-substituted 14-membered macrocyclic lactone ring with conjugated structurally unprecedented diene-ynic side chain terminating at brominated benzene ring. Callyspongiolide showed strong cytotoxicity against human Jurkat J16 T Ramos B lymphocytes.