A5051989421- Cardiovascular Function and Risk Factors
- Diabetes Treatment and Management
- Cardiomyopathy and Myosin Studies
- Chronic Kidney Disease and Diabetes
- Metabolism, Diabetes, and Cancer
- Cardiac Imaging and Diagnostics
- Heart Failure Treatment and Management
- Signaling Pathways in Disease
- Cardiovascular Disease and Adiposity
- Adipose Tissue and Metabolism
- Genetic and Kidney Cyst Diseases
- Coronary Interventions and Diagnostics
- Cardiac Ischemia and Reperfusion
- Advanced Fluorescence Microscopy Techniques
ALK-Abelló (Denmark)
2025
Gubra (Denmark)
2020-2025
University of Copenhagen
2020-2024
Abstract Background Extensive preclinical research aims to develop novel therapeutics for myocardial fibrosis (MF), a condition marked by collagen accumulation that impairs cardiac function. MF is particularly relevant in heart failure with preserved ejection fraction (HFpEF), growing clinical challenge limited treatment options. However, current methods quantifying mouse models struggle accurately capture its heterogeneous regional distribution, creating significant barrier reliably...
Abstract Aim Heart Failure with preserved Ejection Fraction (HFpEF) is characterized by diastolic dysfunction and reduced cardiac output, but its pathophysiology remains poorly understood. Animal models of HFpEF are challenging due to difficulties in assessing the degree heart failure small animals. This study aimed at inducing a mouse model probe preload‐dependency. Methods Increased body mass arterial hypertension were induced mice using Western diet NO synthase inhibition. Preload...
The diabetic heart is characterised by functional, morphological and metabolic alterations predisposing it to contractile failure. Chronic sympathetic activation a feature of the pathogenesis failure, however type 1 shows desensitisation β-adrenergic stimulation. Here, we sought understand impact repeated isoprenaline-mediated β-stimulation upon cardiac mitochondrial respiratory capacity substrate metabolism in 90% pancreatectomy (Px) rat model diabetes. We hypothesised these hearts would be...
Abstract Angiotensin converting enzyme inhibitors, among them captopril, improve survival following myocardial infarction (MI). The mechanisms of captopril action remain inadequately understood due to its diverse effects on multiple signalling pathways at different time periods MI. Here we aimed establish the role in late-stage post-MI remodelling. Left anterior descending artery (LAD) ligation or sham surgery was carried out male C57BL/6J mice. Seven days post-surgery LAD ligated mice were...
Abstract Cardiovascular and renal complications are the predominant causes of morbidity mortality amongst patients with diabetes. Development novel treatments have been hampered by lack available animal models recapitulating human disease. We hypothesized that experimental diabetes in rats combined a cardiac or stressor, would mimic diabetic cardiomyopathy nephropathy, respectively. Diabetes was surgically induced male Sprague Dawley 90% pancreatectomy (Px). Isoprenaline (Iso, 1 mg/kg, sc.,...
The pathogenesis of diabetic cardiomyopathy (DC) is poorly understood and new drugs targeting the myocardium are absent. This may be ascribed to failure available pre-clinical models recapitulate essential clinical features DC heart failure. We hypothesized that combination experimental diabetes pharmacologically induced cardiac stress might provide a novel rat model displaying distinct profile DC. Non-insulin dependent type 1 like mellitus was by partial (90%) pancreatectomy (Px)....
Fibrosis is the hallmark of hypertensive heart disease and failure with preserved ejection fraction. Perivascular fibrosis impairs vascular function while interstitial leads to compromised cardiac contractility. How these types are represented in mouse models what extent transcriptional signatures defined by their location unknown. Mice were dosed over 4 weeks angiotensin II (AngII) alone or together α 1 -adrenergic agonist phenylephrine (PE) characterized echocardiography, light sheet...
Ougaard, Maria K.; Porsgaard, Trine; Sembach, Frederikke E.; Thisted, Louise; Skytte, Jacob L.; Perens, Johanna; Christensen, Michael Author Information
Introduction: Patients with diabetes have an increased risk of cardiovascular disease including myocardial infarction (MI). A major consequence MI is left ventricular (LV) remodeling compensatory hypertrophy without concomitant adaptation the capillary vasculature. Targeting density thus a promising therapeutic strategy but methods for unbiased and direct quantification are lacking. In surgical model, we used 3D light sheet imaging to visualize quantify LV density. Methods: Surgical ligation...
Abstract Background Cardiovascular and renal complications are the predominant causes of morbidity mortality amongst patients with diabetes. Development novel treatments have been hampered by lack available animal models recapitulating human disease. We hypothesized that experimental diabetes in rats combined a cardiac or stressor, would mimic diabetic cardiomyopathy nephropathy, respectively. Methods Diabetes was surgically induced male Sprague Dawley 90% pancreatectomy (Px). Isoprenaline...