The ryanodine receptor (RyR)/Ca2+ release channel is an essential component of excitation–contraction coupling in striated muscle cells. To study the function and regulation Ca2+ channel, we tested effect caffeine on full-length carboxyl-terminal portion skeletal RyR expressed a Chinese hamster ovary (CHO) cell line. Caffeine induced openings full length channels concentration-dependent manner, but it had no channels. CHO cells expressing proteins displayed spontaneous changes intracellular...

For a cystic fibrosis transmembrane conductance regulator (CFTR) channel to enter its open state, serine residues in the R domain must be phosphorylated by cAMP-dependent protein kinase, and intracellular ATP bind nucleotide-binding folds subsequently hydrolyzed. CFTR with partially removed, ΔR(708–835)-CFTR, forms chloride that opens independently of kinase A phosphorylation, probability approximately one-third wild type channel. Deletion this portion from alters response...

Abstract Thylakoid membrane-bound ascorbate peroxidase (tAPX) is a major H 2 O -scavenging enzyme. To clarify its functions in tolerance to rice bacterial blight, we produced lines overexpressing and suppressing tAPX ( OsAPX8 ). The exhibited increased pathogen. RNA interference (RNAi) were considerably more sensitive than the control plant. Further analysis of content these transgenic plants indicated that accumulation -overexpressing was less wild-type RNAi upon challenge with...

The cystic fibrosis transmembrane conductance regulator (CFTR) is a cAMP-dependent protein kinase (PKA)- and ATP-regulated chloride channel, whose gating process involves intra- or intermolecular interactions among the cytosolic domains of CFTR protein. Tandem linkage two molecules produces functional channel with properties that are similar to those native including trafficking plasma membrane, ATP- PKA-dependent gating, unitary 8 picosiemens (pS). A heterodimer, consisting wild type mutant...

We report systematic measurements of anomalous Hall effect (AHE) and spin-orbit torques (SOTs) in MnGa/IrMn films, which a single $L{1}_{0}\text{\ensuremath{-}}\mathrm{MnGa}$ epitaxial layer reveals obvious orbital two-channel Kondo (2CK) effect. As increasing the thickness antiferromagnet IrMn, strong spin (SHE) has gradually suppressed 2CK modified AHE MnGa. A scaling involving multiple competing scattering mechanisms been used to distinguish different contributions AHE. Finally, sizeable...

Disease resistance is an important goal of crop improvement. The molecular mechanism requires further study. Here, we report the identification a rice leaf color mutant, lc7, which defective in chlorophyll synthesis and photosynthesis but confers to Xanthomonas oryzae pv. Oryzae (Xoo). Map-based cloning revealed that lc7 encodes mutant ferredoxin-dependent glutamate synthase1 (Fd-GOGAT1). Fd-GOGAT1 has been proposed have great potential for improving nitrogen-use efficiency, its function...

The cystic fibrosis transmembrane conductance regulator (CFTR) constitutes a linear chloride channel, which is regulated by cAMP-dependent protein kinase phosphorylation at multiple sites located in the intracellular regulatory (R) domain. Studies lipid bilayer system, reported here, provide evidence for control of CFTR channel its R exogenous domain (encoded exon 13 plus 85 base pairs 14) interacted specifically with molecule and inhibited phosphorylation-dependent manner. Only...

Panicle type has a direct bearing on rice yield. Here, we characterized clustered-spikelet mutant, sped1-D, with shortened pedicels and/or secondary branches, which exhibits decreased pollen fertility. We cloned sped1-D and found that it encodes pentatricopeptide repeat protein. investigated the global expression profiles of wild-type, 9311, plants using Illumina RNA sequencing. The several GID1L2 family members was downregulated in suggesting gibberellin (GA) pathway is involved elongation...

We have investigated the topological Hall effect (THE) in MnGa/Pt and MnGa/Ta bilayers induced by interfacial Dzyaloshinskii-Moriya interaction (DMI). The most evident THE signals been found based on MnGa films with small critical DMI energy constant Dc. large portion of signal from total has extracted whole temperature range 5 to 300 K. These results open up exploration magnetic behavior bulk perpendicular anisotropy materials for fundamental physics storage technologies.

ABSTRACT A monoclonal antibody, MPM-13, raised against mitotic HeLa cell extracts recognized a perinuclear area in interphase cells and spindle poles of human, mouse, Chinese hamster sea urchin. On immunoblots MPM-13 major protein band at 43×103Mr variable minor 56 ×103Mr both cells. These antigens were detectable variety mammalian as well the unicellular ciliate Tetrahymena. In arrested mitosis by colcemid stained with indirect immunofluorescence, numerous fluorescent speckles seen...

We report the enhancement of spin-orbit torques (SOT) in MnAl/Ta films with improving chemical ordering through annealing. The switching current density is increased due to enhanced saturation magnetization MS and effective anisotropy field HK after Using harmonic measurements, we have found that both dampling-like HD field-like HF were temperature range 50 300 K, annealing has also value spin Hall angle. improved considered interfacial transparency transmitting MnAl layer. results suggest...

The regulatory (R) domain of the cystic fibrosis transmembrane conductance regulator (CFTR) contains consensus phosphorylation sites for cAMP-dependent protein kinase (PKA) that are basis physiological regulation CFTR chloride channel. A short peptide segment in R with a net negative charge B9 (amino acids 817–838, NEG2) and predicted helical tendency is shown to play critical role channel function. Deletion NEG2 from completely eliminates PKA dependence activity. Exogenous interacts exert...

A negatively charged region of the N-terminal portion skeletal ryanodine receptor (RyR), located between residues 1872-1923, is involved in Ca (2+)-dependent regulation Ca(2+)-release channel. This divergent (RyR1) and cardiac (RyR2) isoforms channel, known as D3. Ca(2+) exerts important regulatory functions on RyR, being both activation inactivation i.e. effects occurring at micromolar millimolar concentrations respectively. To characterize role D3 Ca(2+)-dependent we studied functional...

Perturbation of intracellular Ca2+ homeostasis has been shown to regulate the process cell proliferation and apoptosis. Our previous studies show that mitsugumin 29 (MG29), a synaptophysin-related protein localized in triad junction skeletal muscle, serves an essential role muscle signaling by regulating store-operated entry. Here we report functional interaction between MG29 ryanodine receptor (RyR)/Ca2+ release channel. The purified enhances activity RyR/Ca2+ channel incorporated into...

A negatively charged region of the N-terminal portion skeletal ryanodine receptor (RyR), located between residues 1872–1923, is involved in Ca 2+-dependent regulation Ca2+-release channel. This divergent (RyR1) and cardiac (RyR2) isoforms channel, known as D3. Ca2+ exerts important regulatory functions on RyR, being both activation inactivation i.e. effects occurring at micromolar millimolar concentrations respectively. To characterize role D3 Ca2+-dependent we studied functional...

The effects of spin-orbit coupling and symmetry breaking at the interface between a ferromagnet heavy metal are particularly important for spin-based information storage computation. Recent discoveries suggest they can create novel chiral spin structures (e.g., skyrmions), which have often been identified through appearance bump/dip features Hall signals, so-called topological effect (THE). In this work, however, we present an unusual anomalous (UAHE) in...

Phosphorylation and dephosphorylation of proteins play an important role in the regulation mitosis meiosis. In our previous studies we have described mitosis‐specific monoclonal antibody MPM‐2 that recognizes a family phosphopeptides mitotic cells but not interphase cells. These peptides are synthesized S phase modified by phosphorylation during G 2 /mitosis transition. The epitope for is phosphorylated site. this study, attempted to determine which amino acids ‐mitosis (M) We raised...

The ryanodine receptor/Ca(2+) release channels from skeletal (RyR1) and cardiac (RyR2) muscle cells exhibit different inactivation profiles by cytosolic Ca(2+). D3 is one of the divergent regions between RyR1 (amino acids (aa) 1872-1923) RyR2 (aa 1852-1890) may contain putative binding site(s) for Ca(2+)-dependent RyR. To test this possibility, we have deleted region (DeltaD3-RyR1), residues 1038-3355 (Delta(1038-3355)-RyR2) inserted into Delta(1038-3355)-RyR2 to generate sD3-RyR2. formed...