A5103283188- Pain Mechanisms and Treatments
- Anesthesia and Pain Management
- Nausea and vomiting management
- Hematopoietic Stem Cell Transplantation
- Neuropeptides and Animal Physiology
- Anesthesia and Sedative Agents
- Pain Management and Opioid Use
- Airway Management and Intubation Techniques
- Tracheal and airway disorders
- Nerve injury and regeneration
- Ion channel regulation and function
- Hemoglobinopathies and Related Disorders
- Inflammatory mediators and NSAID effects
- Cardiac, Anesthesia and Surgical Outcomes
- Laser Applications in Dentistry and Medicine
- Pharmacological Effects of Natural Compounds
- Neuroinflammation and Neurodegeneration Mechanisms
- Intensive Care Unit Cognitive Disorders
- Acute Myeloid Leukemia Research
- RNA Interference and Gene Delivery
- Medical and Biological Ozone Research
- Polyomavirus and related diseases
- Foreign Body Medical Cases
- Dental Anxiety and Anesthesia Techniques
- Cytokine Signaling Pathways and Interactions
Chi Mei Medical Center
2020-2025
National Sun Yat-sen University
2003-2025
I-Shou University
2009-2020
E-Da Hospital
2004-2017
Harvard University
2006-2012
Mount Elizabeth Hospital
2006-2012
Brigham and Women's Hospital
2006-2012
Alnylam Pharmaceuticals (United States)
2008
National Yang Ming Chiao Tung University
1998-2007
Kaohsiung Veterans General Hospital
1998-2003
We report the functional characterization of site-directed biotin binding-site mutants recombinant core streptavidin. The mutagenesis studies were aimed at characterizing contributions Trp residues known to contact that have been postulated control exceptional binding affinity observed in this system. properties single replacing with Phe or Ala positions 79, 108, and 120 investigated by quantitating EC50 parameters these 2-iminobiotin an ELISA format. for all was same as wild-type...
Abstract It is well known that interferons (IFNs), such as type-I IFN (IFN-α) and type-II (IFN-γ) are produced by immune cells to elicit antiviral effects. IFNs also glial in the CNS regulate brain functions. As a proinflammatory cytokine, IFN-γ drives neuropathic pain inducing microglial activation spinal cord. However, little about role of IFN-α regulating sensitivity synaptic transmission. Strikingly, we found IFN-α/β receptor (type-I receptor) was expressed primary afferent terminals...
Most cases of the dominantly inherited movement disorder, early onset torsion dystonia (DYT1) are caused by a mutant form torsinA lacking glutamic acid residue in C-terminal region (torsinAΔE). TorsinA is an AAA+ protein located predominantly lumen endoplasmic reticulum (ER) and nuclear envelope apparently involved membrane structure/movement processing proteins through secretory pathway. A reporter Gaussia luciferase (Gluc) shows reduced rate secretion primary fibroblasts from DYT1 patients...
We evaluated the effect of a single dose dexamethasone on incidence and severity postoperative nausea vomiting (PONV) after intrathecal injection tetracaine plus neostigmine. Sixty ASA physical status I patients scheduled for inguinal herniorrhaphy were studied with randomized, double-blinded, placebo-controlled protocol. The group (Group D) received 10 mg IV before performance spinal anesthesia, whereas placebo P) saline. Spinal anesthesia was performed 15 neostigmine 100 μg in both groups....
Despite decades of intense research efforts, actions acute opioids are not fully understood. Increasing evidence suggests that in addition to well-documented antinociceptive effects also produce paradoxical hyperalgesic and excitatory on neurons. However, most studies focus the pronociceptive chronic opioid exposure. Matrix metalloproteinase 9 (MMP-9) plays an important role neuroinflammation neuropathic pain development. We examined MMP-9 expression localization dorsal root ganglia (DRGs)...
Background: Histamine receptors are involved in the development of inflammatory pain and hyperalgesia, use antihistamines is advocated as an alternative for therapy treatment postoperative nausea vomiting. We investigated influence timing promethazine administration on outcomes. Methods: Ninety female patients undergoing total abdominal hysterectomy were randomly divided into three groups. All individuals received infusions normal saline before anaesthesia induction, postoperatively Pre...
N-methyl-D-aspartate and other glutamate receptors have been shown to present on the peripheral axons of primary afferents, injection N-methyl-D-aspartate-receptor antagonists can suppress hyperalgesia allodynia. Thus, this study examined postoperative analgesic adverse effects local ketamine administered postoperatively.Ketamine (0.3%, 3 mL) or saline was subcutaneously infiltrated before incision in a double-blind manner using sample population 40 patients undergoing circumcision surgery,...
Background & AimsMolecular mechanisms underlying the activated spinal microglia in association with pain chronic pancreatitis (CP) remain unknown. We tested whether P2X7R on mediates pathogenesis of visceral using a CP rat model.MethodsThe model was induced via intraductal injection 2% trinitrobenzene sulfonic acid into male Sprague-Dawley rats. Hyperalgesia assessed based mechanical sensitivity to Von-Frey filaments (VFFs), and nocifensive behaviors were measured response electrical...
Background Spinal N-methyl-D-aspartate receptors have been demonstrated to play an important role in the facilitation and maintenance of nociception. To avoid adverse effects blocking central nervous system, receptor peripheral system is ideal alternative. Transfection small interfering RNAs (siRNAs) into cells has revealed provide potent silencing specific genes. In this study, authors examined effect subcutaneous injection siRNA targeting NR1 subunit on gene expression subsequently...