Wei‐Hung Chen
A5103267938- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- COVID-19 Impact on Reproduction
- Myasthenia Gravis and Thymoma
- Animal Virus Infections Studies
- Force Microscopy Techniques and Applications
- Peripheral Neuropathies and Disorders
- Acute Ischemic Stroke Management
- SARS-CoV-2 detection and testing
- Gold and Silver Nanoparticles Synthesis and Applications
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Polyoxometalates: Synthesis and Applications
- Microwave-Assisted Synthesis and Applications
- Mesoporous Materials and Catalysis
- Migraine and Headache Studies
- Conducting polymers and applications
- Transcranial Magnetic Stimulation Studies
- Autoimmune Neurological Disorders and Treatments
- Viral gastroenteritis research and epidemiology
- Complement system in diseases
- Shoulder Injury and Treatment
- Mechanical Circulatory Support Devices
- Long-Term Effects of COVID-19
- Cerebrovascular and Carotid Artery Diseases
Henry M. Jackson Foundation
2020-2025
Walter Reed Army Institute of Research
2020-2025
Peking University
2024
State Key Laboratory of Artificial Microstructure and Mesoscopic Physics
2024
Taipei Medical University
2005-2023
National Taiwan University
2001-2023
National Defense Medical Center
2023
Taichung Armed Forces General Hospital
2023
Tri-Service General Hospital
2023
E-Da Hospital
2015-2022
The need for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) next-generation vaccines has been highlighted by the rise of variants concern (VoCs) and long-term threat emerging coronaviruses. Here, we design characterize four categories engineered nanoparticle immunogens that recapitulate structural antigenic properties prefusion SARS-CoV-2 spike (S), S1, receptor-binding domain (RBD). These induce robust S binding, ACE2 inhibition, authentic pseudovirus neutralizing antibodies...
The emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants stresses the continued need for next-generation vaccines that confer broad protection against disease 2019. We developed and evaluated an adjuvanted SARS-CoV-2 spike ferritin nanoparticle (SpFN) vaccine in nonhuman primates. High-dose (50-μg) SpFN vaccine, given twice 28 days apart, induced a T helper cell 1 (T H 1)–biased CD4 response elicited neutralizing antibodies wild type concern, as well SARS-CoV-1....
Epstein-Barr virus (EBV) causes infectious mononucleosis and is associated with malignancies in humans. Viral infection of B cells initiated by the viral glycoprotein 350 (gp350) binding to complement receptor 2 (CR2). Despite decades effort, no vaccines or curative agents have been developed, partly due lack atomic-level understanding virus-host interface. Here, we determined 1.7 Å structure gp350 complex CR2. CR2 utilized same set Arg residues required for recognition its natural ligand,...
Abstract The emergence of variants concern, some with reduced susceptibility to COVID-19 vaccines underscores consideration for the understanding vaccine design that optimizes induction effective cellular and humoral immune responses. We assessed a SARS-CoV-2 spike-ferritin nanoparticle (SpFN) immunogen paired two distinct adjuvants, Alhydrogel ® or Army Liposome Formulation containing QS-21 (ALFQ) unique evoked signatures. Recruitment highly activated multifaceted antigen-presenting cells...
Abstract The emergence of SARS-CoV-2 variants concern (VOC) requires adequate coverage vaccine protection. We evaluated whether a spike ferritin nanoparticle (SpFN), adjuvanted with the Army Liposomal Formulation QS21 (ALFQ), conferred protection against Alpha (B.1.1.7), and Beta (B.1.351) VOCs in Syrian golden hamsters. SpFN-ALFQ was administered as either single or double-vaccination (0 4 week) regimens, using high (10 μg) low (0.2 dose. Animals were intranasally challenged at week 11....
Emergence of novel variants the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) underscores need for next-generation vaccines able to elicit broad and durable immunity. Here we report evaluation a ferritin nanoparticle vaccine displaying receptor-binding domain SARS-CoV-2 spike protein (RFN) adjuvanted with Army Liposomal Formulation QS-21 (ALFQ). RFN vaccination macaques using two-dose regimen resulted in robust, predominantly Th1 CD4+ T cell responses reciprocal peak mean...
Despite rapid and ongoing vaccine therapeutic development, SARS-CoV-2 continues to evolve evade, presenting a need for next-generation diverse modalities. Here we show that nurse sharks immunized with recombinant receptor binding domain (RBD), RBD-ferritin (RFN), or spike protein ferritin nanoparticle (SpFN) immunogens elicit set of new antigen antibody (IgNAR) molecules target two non-overlapping conserved epitopes on the RBD. Representative shark variable NAR-Fc chimeras (ShAbs) targeting...
Abstract The repeat emergence of SARS-CoV-2 variants concern (VoC) with decreased susceptibility to vaccine-elicited antibodies highlights the need develop next-generation vaccine candidates that confer broad protection. Here we describe antibody response induced by Spike Ferritin Nanoparticle (SpFN) candidate adjuvanted Army Liposomal Formulation including QS21 (ALFQ) in non-human primates. By isolating and characterizing several monoclonal directed against Receptor Binding Domain (RBD),...
Nonribosomal peptide synthetases are large, complex multidomain enzymes responsible for the biosynthesis of a wide range peptidic natural products. Inherent to synthetase chemistry is thioester templated mechanism that relies on protein/protein interactions and interdomain dynamics. Several questions related structure remain be addressed, including incorporation accessory domains intermodule interactions. The inclusion nonproteinogenic d-amino acids into frameworks common important...
Prevention of viral escape and increased coverage against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants concern require therapeutic monoclonal antibodies (mAbs) targeting multiple sites vulnerability on the spike glycoprotein. Here we identify several potent neutralizing directed either N-terminal domain (NTD) or receptor-binding (RBD) protein. Administered in combinations, these mAbs provided low-dose protection SARS-CoV-2 infection K18-human angiotensin-converting...
SUMMARY SARS-CoV-2 is a zoonotic virus that has caused pandemic of severe respiratory disease—COVID-19— within several months its initial identification. Comparable to the first SARS-CoV, this novel coronavirus’s surface Spike (S) glycoprotein mediates cell entry via human ACE-2 receptor, and, thus, principal target for development vaccines and immunotherapeutics. Molecular information on S remains limited. Here we report crystal structure receptor-binding-domain (RBD) at highest resolution...
The COVID-19 pandemic has had a staggering impact on social, economic, and public health systems worldwide. Vaccine development mobilization against SARS-CoV-2 (the etiologic agent of COVID-19) been rapid. However, novel strategies are still necessary to slow the pandemic, this includes new approaches vaccine and/or delivery that will improve vaccination compliance demonstrate efficacy emerging variants. Here, we report immunogenicity comprising stabilized, pre-fusion spike protein trimers...
Given the continuous emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants concern (VoCs), immunotherapeutics that target conserved epitopes on spike (S) glycoprotein have therapeutic advantages. Here, we report crystal structure SARS-CoV-2 S receptor-binding domain (RBD) at 1.95 Å and describe flexibility distinct conformations angiotensin-converting enzyme (ACE2)-binding site. We identify a set SARS-CoV-2-reactive monoclonal antibodies (mAbs) with broad RBD...
The reduction of epoxyqueuosine (oQ) is the last step in synthesis tRNA modification queuosine (Q). While reductase (EC 1.17.99.6) enzymatic activity was first described 30 years ago, encoding gene queG only identified Escherichia coli 2011. Interestingly, absent from a large number sequenced genomes that harbor Q or salvage genes, suggesting existence an alternative these organisms. By analyzing phylogenetic distributions, physical clustering, and fusions, members Domain Unknown Function...
This study demonstrates the impact of adjuvant on development T follicular helper (Tfh) and B cells, their influence antibody responses in mice vaccinated with SARS-CoV-2-spike-ferritin-nanoparticle (SpFN) adjuvanted either Army Liposome Formulation containing QS-21 (SpFN + ALFQ) or Alhydrogel
Ischemic stroke, the most prevalent form of leads to neurological impairment due cerebral ischemia and affects 55-90% population. Brain-derived neurotrophic factor (BDNF) plays a crucial role in central nervous system regulates cardiometabolic risk factors, including lipids. This single-center study aimed explore relationship between lipid profiles BDNF levels 90 patients who had experienced AIS for first time. The results show that high group (≥3.227 ng/mL) significantly higher HbA1C TG...
Sjögren's syndrome (SS) is an important but poorly recognized cause of peripheral neuropathy. Several forms nerve dysfunction occur, including trigeminal sensory neuropathy, mononeuropathy multiplex, distal sensorimotor polyneuropathy and pure neuronopathy. The pathological findings vary the definite treatment not known. Here we present 4 cases acute ataxic with SS, experience plasmapheresis (PP). patients were all females; ages ranged from 30 to 58 years. All had prominent loss kinesthetic...
Colchicine and 3-hydroxy-3-methy-glutaryl coenzyme A (HMG-CoA) reductase inhibitors are well known to cause myopathy. Myotoxicity is dose-dependent in both drugs; therefore, the onset of symptoms usually takes months or years. We report case a patient with chronic renal failure who had been taking simvastatin for 2 years developed acute weakness weeks after start treatment colchicines recurrent gout. The electromyography elevated muscle enzymes indicated that his were caused by When this...
Our objective was to investigate the risk of coronary heart disease (CHD) in patients with Hashimoto's thyroiditis (HT). The Taiwan National Health Insurance Research Database used conduct a retrospective cohort analysis. study consisted 1165 newly diagnosed HT and 4660 matched controls (non-HT patients) from 2000 2010. median follow-up time 5.46 years. developing CHD for measured using Cox proportional hazards model. increased compared non-HT controls, an adjusted hazard ratio (HR) 1.44...