A5001700139- T-cell and B-cell Immunology
- Aquaculture disease management and microbiota
- Immune Cell Function and Interaction
- SARS-CoV-2 and COVID-19 Research
- Influenza Virus Research Studies
- Immunotherapy and Immune Responses
- Invertebrate Immune Response Mechanisms
- SARS-CoV-2 detection and testing
- Respiratory viral infections research
- Glycosylation and Glycoproteins Research
- Atherosclerosis and Cardiovascular Diseases
- interferon and immune responses
- Protein purification and stability
- vaccines and immunoinformatics approaches
- Viral gastroenteritis research and epidemiology
- Immune Response and Inflammation
- Animal Disease Management and Epidemiology
- RNA and protein synthesis mechanisms
- Animal Virus Infections Studies
- Bacterial Genetics and Biotechnology
- Monoclonal and Polyclonal Antibodies Research
- Escherichia coli research studies
- Immune responses and vaccinations
- Cell Adhesion Molecules Research
- Identification and Quantification in Food
Washington University in St. Louis
2023-2025
University of Maryland, Baltimore
2016-2023
Institut de Ciències del Mar
2022
Despite rapid and ongoing vaccine therapeutic development, SARS-CoV-2 continues to evolve evade, presenting a need for next-generation diverse modalities. Here we show that nurse sharks immunized with recombinant receptor binding domain (RBD), RBD-ferritin (RFN), or spike protein ferritin nanoparticle (SpFN) immunogens elicit set of new antigen antibody (IgNAR) molecules target two non-overlapping conserved epitopes on the RBD. Representative shark variable NAR-Fc chimeras (ShAbs) targeting...
The absence of germinal centers (GCs) in cartilaginous fishes lies at odds with data showing that nurse sharks can produce robust antigen-specific responses and affinity mature their B cell repertoires. To investigate this apparent incongruity, we performed RNA sequencing on single nuclei, allowing us to characterize the types present shark spleen, RNAscope provide situ cellular resolution key marker gene expression following immunization R-phycoerythrin (PE). We tracked PE splenic follicles...
Seasonal influenza viruses cause significant global illness and death annually, the potential spillover of avian H5N1 poses a serious pandemic threat. Traditional vaccines target variable hemagglutinin (HA) protein, necessitating annual vaccine updates, while slower-evolving neuraminidase (NA) presents promising for broader protection. We investigated breadth anti-NA B cell responses to seasonal vaccination in humans. screened plasmablast-derived monoclonal antibodies (mAbs) from three...
As of 8 August 2022, SARS-CoV-2, the causative agent COVID-19, has infected over 585 million people and resulted in more than 6.42 deaths worldwide. While approved SARS-CoV-2 spike (S) protein-based vaccines induce robust seroconversion most individuals, dramatically reducing disease severity risk hospitalization, poorer responses are observed aged, immunocompromised individuals patients with certain pre-existing health conditions. Further, it is difficult to predict protection conferred...
Eliciting broad and durable antibody responses against rapidly evolving pathogens like influenza viruses remains a formidable challenge
Clostridium difficile is the leading cause of nosocomial infections in United States, adding billions dollars per year to health care costs.A vaccine targeted against bacterium would be extremely beneficial decreasing morbidity and mortality caused by C. difficile-associated disease; a directed colonization factor hinder spread as well prevent disease.Type IV pili (T4Ps) are extracellular appendages composed protein monomers called pilins.They involved adhesion wide variety bacteria archaea,...
Many animals of scientific importance lack species-specific reagents (e.g., monoclonal antibodies) for in-depth studies immune proteins. Mass spectrometry (MS)-based proteomics has emerged as a useful method monitoring changes in protein abundance and modifications non-model species. It can be used to quantify hundreds candidate molecules simultaneously without the generation new reagents. Here, we MS-based identify proteins plasma nurse shark ( Ginglymostoma cirratum ), cartilaginous fish...
As COVID-19 pandemic public health measures are easing globally, the emergence of new SARS-CoV-2 strains continue to present high risk for vulnerable populations. The antibody-mediated protection acquired from vaccination and/or infection is seen wane over time and immunocompromised populations can no longer expect benefit monoclonal antibody prophylaxis. Hence, there a need monitor variants its effect on vaccine performance. In this context, surveillance infections serology testing gaining...
Abstract Sharks are members of the oldest extant taxonomic group (Chondrichthyes) to have immunoglobulin (Ig)-based adaptive immunity. While research has demonstrated that sharks can produce antigen-specific antibody responses, affinity mature these antibodies, and establish immunological memory, histological examination so far failed find evidence germinal centers (GCs). To explore this apparent discrepancy, we immunized several nurse (Ginglymostoma cirratum) with phycoerythrin visualized...