A5009560249- SARS-CoV-2 and COVID-19 Research
- Respiratory viral infections research
- COVID-19 Clinical Research Studies
- Invertebrate Immune Response Mechanisms
- Viral Infectious Diseases and Gene Expression in Insects
- Viral gastroenteritis research and epidemiology
- Immune Cell Function and Interaction
- Animal Virus Infections Studies
- Pneumonia and Respiratory Infections
- Immunotherapy and Immune Responses
- COVID-19 Impact on Reproduction
- Influenza Virus Research Studies
- Neonatal Respiratory Health Research
- RNA Research and Splicing
- HIV Research and Treatment
- Viral Infections and Immunology Research
- Long-Term Effects of COVID-19
- Immune Response and Inflammation
- Cytokine Signaling Pathways and Interactions
- Insect Resistance and Genetics
- Silkworms and Sericulture Research
- CRISPR and Genetic Engineering
- FOXO transcription factor regulation
- RNA Interference and Gene Delivery
- Genetics, Aging, and Longevity in Model Organisms
Uniformed Services University of the Health Sciences
2021-2025
Deyang Stomatological Hospital
2024-2025
Henry M. Jackson Foundation
2021-2025
Southwest University
2018-2024
Unifor
2023
Jackson Foundation
2023
State Key Laboratory of Silkworm Genomic Biology
2018-2022
National Cancer Institute
2018-2020
Center for Cancer Research
2018-2020
Frederick National Laboratory for Cancer Research
2018-2020
Abstract The emergence of variants concern, some with reduced susceptibility to COVID-19 vaccines underscores consideration for the understanding vaccine design that optimizes induction effective cellular and humoral immune responses. We assessed a SARS-CoV-2 spike-ferritin nanoparticle (SpFN) immunogen paired two distinct adjuvants, Alhydrogel ® or Army Liposome Formulation containing QS-21 (ALFQ) unique evoked signatures. Recruitment highly activated multifaceted antigen-presenting cells...
Sapoviruses (SaVs) are enteric caliciviruses that have been detected in multiple mammalian species, including humans, pigs, mink, dogs, sea lions, chimpanzees, and rats. They show a high level of diversity. A SaV genome commonly encodes seven nonstructural proteins (NSs), the RNA polymerase protein NS7, two structural (VP1 VP2). We classified human animal SaVs into 15 genogroups (G) based on available VP1 sequences, three newly characterized genomes from this study. sequenced full length one...
We compare immunogenicity and protective efficacy of an HIV vaccine comprised env gag DNA Env (Envelope) proteins by co-administration the components in same muscles or separate administration + protein contralateral sites female rhesus macaques. The 6-valent includes gp145 DNAs, representing six sequentially isolated Envs from HIV-infected individual CH505, matching GLA-SE-adjuvanted gp120 proteins. Interestingly, only macaques group are protected against SHIV CH505 acquisition after...
Characterizing the longevity and quality of cellular immune responses to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) enhances understanding disease 2019 (COVID-19) immunity that influences clinical outcomes. Prior studies suggest SARS-CoV-2-specific T cells are present in peripheral blood 10 months after infection. Analysis function, durability, diversity response long natural infection, over a range ages phenotypes, is needed identify preventative therapeutic...
This study demonstrates the impact of adjuvant on development T follicular helper (Tfh) and B cells, their influence antibody responses in mice vaccinated with SARS-CoV-2-spike-ferritin-nanoparticle (SpFN) adjuvanted either Army Liposome Formulation containing QS-21 (SpFN + ALFQ) or Alhydrogel
Hepatic insulin resistance (IR) is associated with liver inflammatory diseases, but molecular mechanisms for the association remained elusive. IR known to increase activity of forkhead box-containing protein O subfamily-1 (FOXO1), a transcription factor that was recently shown enhance proinflammatory cytokine production in macrophages and adipocytes. Here we report overexpression constitutively active FOXO1 markedly increased chemokine ligand 20 (CCL20) expression secretion HepG2 hepatoma...
Abstract Background T cells are associated with viral clearance and improved outcomes in COVID-19, but also immunopathology. Defining cell diversity response to SARS-CoV-2 infection enables implementation of prevention treatment strategies. recognize peptide sequences from pathogens upon presentation major histocompatibility complexes (MHC). Human MHC molecule results a wide array recognition by making escape difficult, challenging measure. Methods Using the ImmunoSEQ Assay sequence...
Abstract Purpose People with both hypertension and coronary artery disease (CAD) are at a significantly increased risk of major adverse cardiovascular events (MACEs). This study aimed to develop validate combination model that integrates radiomics features pericoronary adipose tissue (PCAT), CT-derived fractional flow reserve (CT-FFR), clinicoradiological features, which improves MACE prediction within two years. Materials methods Coronary-computed tomography angiography data were gathered...
Abstract Ionizable lipid nanoparticles (LNP) that have enabled the success of messenger RNA (mRNA) vaccines been shown to be immunostimulatory in absence mRNA. However, mechanisms through which they activate innate immune cells is incompletely understood. Using a monocyte cell line, we compared ability three LNP formulations transcription factors Nuclear Factor-kappa B (NF-κB) and Interferon Regulatory Factor (IRF). Comparison signaling knockout lines illustrated role for Toll-like receptor...
Young infants frequently experience respiratory tract infections, yet vaccines designed to provide mucosal protection are lacking. Localizing pathogen-specific cellular and humoral immune responses the lung could improved protection. We used a well-characterized murine model of syncytial virus (RSV) study development lung-resident memory T cells (T
The porcine sapovirus (SaV) (PoSaV) Cowden strain is one of only a few culturable enteric caliciviruses. Compared to the wild-type (WT) PoSaV strain, tissue culture-adapted (TC) has two conserved amino acid substitutions in RNA-dependent RNA polymerase (RdRp) and six capsid protein (VP1). By using reverse-genetics system, we identified that 4 VP1 (residues 178, 289, 324, 328), but not RdRp region, were critical for cell culture adaptation strain. other 291 295) reduced virus replication...
HIV sequence diversity and the propensity of eliciting immunodominant responses targeting inessential variable regions are hurdles in development an effective AIDS vaccine. We developed a DNA vaccine comprising conserved elements (CE) SIV p27Gag HIV-1 Env found that priming vaccination with CE is critical to efficiently overcome dominance imposed by Gag regions. Here, we show vaccinated macaques receiving prime/CE+full-length co-delivery booster regimens broad, potent durable cytotoxic T...
Abstract Potent cellular responses to viral infections are pivotal for long -lived protection. Evidence is growing that these critical in SARS -CoV-2 immunity. Assessment of a spike ferritin nanoparticle (SpFN) immunogen paired with two distinct adjuvants, Alhydrogel® (AH) or Army Liposome Formulation containing QS-21 (ALFQ) demonstrated unique vaccine evoked immune signatures. SpFN+ALFQ enhanced recruitment highly activated classical and non -classical antigen presenting cells (APCs) the...
Abstract Background Characterizing the longevity and quality of cellular immune responses to SARS-CoV-2 is critical understanding immunologic approaches protection against COVID-19. Prior studies suggest SARS-CoV-2-specific T cells are present in peripheral blood 10 months after infection. Further analysis function, durability, diversity response long natural infection, over a wider range ages disease phenotypes, needed further identify preventative therapeutic interventions. Methods We...
Abstract Lipid nanoparticles (LNP) are essential components of messenger RNA (mRNA) vaccines that have been a cornerstone the COVID-19 public health response. These LNPs used for cell entry and protection mRNA. Others shown LNP BNT162b2 vaccine is able to augment immune response against target. However, it unknown how this stimulates innate cells such as monocytes participate in engagement adaptive We THP-1 monocyte reporter line nuclear factor kappa B (NF-κB) interferon regulatory (IRF)...
Frequencies and phenotypes of immune cells differ between neonates adults in association with age-specific responses. Lymph nodes (LN) are critical tissue sites to quantify define these differences. Advances flow cytometry have enabled more multifaceted measurements complex Tissue processing can affect the under investigation that influence key findings. To understand impact on LN after for single-cell suspension, we compared three dissociation protocols: enzymatic digestion, mechanical...