Nusrat J Epsi
A5007323855- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- Long-Term Effects of COVID-19
- SARS-CoV-2 detection and testing
- vaccines and immunoinformatics approaches
- Prostate Cancer Treatment and Research
- COVID-19 and healthcare impacts
- Animal Virus Infections Studies
- Inflammasome and immune disorders
- Cancer Genomics and Diagnostics
- Vaccine Coverage and Hesitancy
- COVID-19 and Mental Health
- Respiratory viral infections research
- Bioinformatics and Genomic Networks
- Lung Cancer Treatments and Mutations
- Influenza Virus Research Studies
- Prostate Cancer Diagnosis and Treatment
- Bacillus and Francisella bacterial research
- COVID-19 epidemiological studies
- Travel-related health issues
- Tryptophan and brain disorders
- Viral Infections and Immunology Research
- Biosensors and Analytical Detection
- Epigenetics and DNA Methylation
- Fibromyalgia and Chronic Fatigue Syndrome Research
HJF Medical Research International
2021-2025
Henry M. Jackson Foundation
2020-2024
Uniformed Services University of the Health Sciences
2020-2024
Rutgers, The State University of New Jersey
2017-2020
Rutgers Health
2018-2020
Jackson Foundation
2020
Unifor
2020
The rapid spread of the highly contagious Omicron variant severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) along with its high number mutations in spike gene has raised alarms about effectiveness current medical countermeasures. To address this concern, we measured neutralization BA.1 pseudovirus by postvaccination serum samples after two and three immunizations Pfizer/BioNTech162b2 SARS-CoV-2 mRNA (Pfizer/BNT162b2) vaccine, convalescent from unvaccinated individuals infected...
Importance Understanding the factors associated with post-COVID conditions is important for prevention. Objective To identify characteristics persistent post–COVID-19 symptoms and to describe medical encounters. Design, Setting, Participants This cohort study used data from Epidemiology, Immunology, Clinical Characteristics of Emerging Infectious Diseases With Pandemic Potential (EPICC) implemented in US military health system (MHS); MHS beneficiaries aged 18 years or older who tested...
The rapid emergence of SARS-CoV-2 variants challenges vaccination strategies. Here, we collected 201 serum samples from persons with a single infection or multiple vaccine exposures, both. We measured their neutralization titers against 15 natural and 7 engineered spike mutations analyzed antigenic diversity. Antigenic maps primary sera showed that Omicron sublineages BA.2, BA.4/BA.5, BA.2.12.1 are distinct BA.1 more similar to Beta/Gamma/Mu variants. Three mRNA COVID-19 vaccinations...
Abstract Background Comparison of humoral responses in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccinees, those with SARS-CoV-2 infection, or combinations vaccine/ infection (“hybrid immunity”) may clarify predictors vaccine immunogenicity. Methods We studied 2660 US Military Health System beneficiaries a history infection-alone (n = 705), vaccination-alone 932), vaccine-after-infection 869), and vaccine-breakthrough-infection 154). Peak anti-spike–immunoglobulin G (IgG)...
The rapid spread of the highly contagious Omicron variant SARS-CoV-2 along with its high number mutations in spike gene has raised alarm about effectiveness current medical countermeasures. To address this concern, we measured neutralizing antibodies against three important settings: (1) post-vaccination sera after two and immunizations Pfizer/BNT162b2 vaccine, (2) convalescent from unvaccinated individuals infected by different variants, (3) clinical-stage therapeutic antibodies. Using a...
Characterizing the longevity and quality of cellular immune responses to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) enhances understanding disease 2019 (COVID-19) immunity that influences clinical outcomes. Prior studies suggest SARS-CoV-2-specific T cells are present in peripheral blood 10 months after infection. Analysis function, durability, diversity response long natural infection, over a range ages phenotypes, is needed identify preventative therapeutic...
Sensitive and specific SARS-CoV-2 antibody assays remain critical for community hospital-based sero-surveillance. With the rollout of vaccines, such must be able to distinguish vaccine from natural immunity related human coronaviruses. Here, we developed implemented multiplex microsphere-based immunoassay strategies COVD-19 studies that incorporates spike protein trimers endemic seasonal coronaviruses (HCoV), enabling high throughout measurement pre-existing cross-reactive antibodies. We...
Master regulatory genes of tissue specification play key roles in stem/progenitor cells and are often important cancer. In the prostate, androgen receptor (AR) is a master regulator essential for development tumorigenesis, but its specific functions prostate have not been elucidated. We investigated AR function CARNs (CAstration-Resistant Nkx3.1-expressing cells), luminal cell that regeneration. Using genetically--engineered mouse models novel epithelial lines, we find progenitor properties...
BackgroundPrioritization of breast cancer patients based on the risk resistance to tamoxifen plays a significant role in personalized therapeutic planning and improving disease course outcomes.MethodsIn this work, we demonstrate that genome-wide pathway-centric computational framework elucidates molecular pathways as markers ER+ patients. In particular, associated activity levels with wide spectrum response tamoxifen, which defined cancer.FindingsWe identified five biological failure...
Background Accurate COVID-19 prognosis is a critical aspect of acute and long-term clinical management. We identified discrete clusters early stage-symptoms which may delineate groups with distinct disease severity phenotypes, including risk developing symptoms associated inflammatory profiles. Methods 1,273 SARS-CoV-2 positive U.S. Military Health System beneficiaries quantitative symptom scores (FLU-PRO Plus) were included in this analysis. employed machine-learning approaches to identify...
Abstract Background Post-COVID conditions (PCC, ‘Long COVID’) remain a military health concern, and symptom heterogeneity limits understanding of PCC pathogenesis. To address this, we used machine learning to define phenotypes in Military Health System (MHS) beneficiaries. Here, extend these findings identify early transcriptomic profiles which may predict the development distinct symptom-based phenotypes. (A) Machine techniques identified three groups participants based on chronic...
Abstract Background SARS-CoV-2 myocarditis is a rare but serious complication of COVID-19. The host response correlates remain unclear, thereby limiting therapeutic development. We therefore performed proteomic and transcriptomic phenotyping in Military Health System (MHS) beneficiaries. included bioenergetics focus given the emerging role mitochondrial function enteroviral myocarditis. Early (<30 days post infection) plasma biomarkers measured by Ella 20-plex ELISA assay cases...
ABSTRACT Importance The persistence of SARS-CoV-2 antibodies may be a predictive correlate protection for both natural infections and vaccinations. Identifying predictors robust antibody responses is important to evaluate the risk re-infection / vaccine failure translatable effectiveness. Objective To 1) determine durability anti-SARS-CoV-2 IgG neutralizing in subjects who experienced mild moderate severe COVID-19, 2) correlation age endemic human seasonal coronaviruses (HCoVs) according...
Abstract Antigenic assessments of SARS-CoV-2 variants inform decisions to update COVID-19 vaccines. Primary infection sera are often used for assessments, but such rare due population immunity from infections and vaccinations. Here, we show that neutralization titers breadth matched human hamster pre-Omicron variant primary correlate well generate similar antigenic maps. The map shows modest drift among XBB sub-lineage variants, with JN.1 BA.4/BA.5 within the cluster, five six-fold...
Abstract Background Post-COVID conditions (PCC) are difficult to characterize, diagnose, predict, and treat due overlapping symptoms poorly understood pathology. Identifying inflammatory profiles may improve clinical prognostication trial endpoints. Methods This analysis included 1988 SARS-CoV-2 positive U.S. Military Health System beneficiaries who had quantitative post–COVID symptom scores. Among participants reported moderate-to-severe on surveys collected 6 months post-SARS-CoV-2...
ABSTRACT Antigenic assessments of SARS-CoV-2 variants inform decisions to update COVID-19 vaccines. Primary infection sera are often used for assessments, but such rare due population immunity from infections and vaccinations. Here, we show that neutralization titers breadth matched human hamster pre-Omicron variant primary correlate well generate similar antigenic maps. The map shows modest drift among XBB sub-lineage variants, with JN.1 BA.4/BA.5 within the cluster, fivefold sixfold...
Lethal COVID-19 outcomes are attributed to classic cytokine storm. We revisit this using RNA sequencing of nasopharyngeal and 40 autopsy samples from patients dying SARS-CoV-2. Subsets the 100 top-upregulated genes in nasal swabs upregulated heart, lung, kidney, liver, but not mediastinal lymph nodes. Twenty-two these “noncanonical” immune genes, which we link components renin-angiotensin-activation-system that manifest as increased fibrin deposition, leaky vessels, thrombotic tendency,...
Abstract Background Coronavirus disease 2019 (COVID-19) presents a unique challenge to United States Navy hospital ships. The aim of this study was determine the prevalence severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection among US personnel deployed on USNS COMFORT augment inpatient health care capacity in New York City. Methods This cross-sectional conducted crewmembers returning Norfolk, Virginia, following deployment. Participants completed an electronic...
Abstract Background The inFLUenza Patient-Reported Outcome Plus (FLU-PRO Plus) is a patient-reported outcome data collection instrument assessing symptoms of viral respiratory tract infections across 8 body systems. This study evaluated the measurement properties FLU-PRO in enrolling individuals with coronavirus disease 2019 (COVID-19). Methods Data from prospective cohort (EPICC) US Military Health System beneficiaries for COVID-19 was utilized. Adults symptomatic severe acute syndrome 2...
Androgen receptor (AR) plays a fundamental role in most aspects of adult prostate homeostasis, and anti-androgen therapy represents the cornerstone cancer treatment. However, early organogenesis takes place during pre-pubertal stages when androgen levels are low, raising possibility that AR function is more limited development. Here, we use inducible deletion lineage tracing genetically engineered mice to show basal luminal epithelial progenitors do not require cell-autonomous activity We...
Abstract Background The mechanisms underlying the association between obesity and coronavirus disease 2019 (COVID-19) severity remain unclear. After verifying that was a correlate of severe COVID-19 in US Military Health System (MHS) beneficiaries, we compared immunological virological phenotypes acute respiratory syndrome 2 (SARS-CoV-2) infection both obese nonobese participants. Methods COVID-19–infected MHS beneficiaries were enrolled, anthropometric, clinical, demographic data collected....
Abstract Lethal COVID-19 outcomes are most often attributed to classic cytokine storm and attendant excessive immune signaling. We re-visit this question using RNA sequencing in nasopharyngeal 40 autopsy samples from COVID-19-positive negative individuals. In nasal swabs, the top 100 genes which significantly correlated with viral load, include many canonical innate genes. However, 22 much less studied "non-canonical" found despite absence of transcripts, subsets these upregulated heart,...
Therapeutic resistance is a central problem in clinical oncology. We have developed systematic genome-wide computational methodology to allow prioritization of patients with favorable and poor therapeutic response. Our method, which integrates DNA methylation mRNA expression data, uncovered panel 5 differentially methylated sites, explain changes their site-harboring genes, demonstrated ability predict primary androgen-deprivation therapy (ADT) the TCGA prostate cancer patient cohort (hazard...
Patient-reported outcomes of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection are an important measure the full burden disease (COVID). Here, we examine how (1) infecting genotype and COVID-19 vaccination correlate with inFLUenza Patient-Reported Outcome (FLU-PRO) Plus score, including by symptom domains, (2) FLU-PRO scores predict return to usual activities health.The epidemiology, immunology, clinical characteristics pandemic infectious diseases (EPICC) study was...
Despite recent advances in discovering a wide array of novel chemotherapy agents, identification patients with poor and favorable response prior to treatment administration remains major challenge clinical oncology. To tackle this challenge, we present generalizable genome-wide computational framework pathCHEMO that uncovers interplay between transcriptomic epigenomic mechanisms altered biological pathways govern cancer patients. Our approach is tested on lung adenocarcinoma who received...