A5007960764- Single-cell and spatial transcriptomics
- SARS-CoV-2 and COVID-19 Research
- Cancer Cells and Metastasis
- Cancer Genomics and Diagnostics
- RNA Research and Splicing
- Heat shock proteins research
- Advanced biosensing and bioanalysis techniques
- Viral gastroenteritis research and epidemiology
- COVID-19 Clinical Research Studies
- Melanoma and MAPK Pathways
- interferon and immune responses
- FOXO transcription factor regulation
- Phagocytosis and Immune Regulation
- Gene Regulatory Network Analysis
Max Delbrück Center
2020-2023
Detailed knowledge of the molecular biology severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is crucial for understanding viral replication, host responses, and disease progression. Here, we report gene expression profiles three SARS-CoV- SARS-CoV-2-infected human cell lines. SARS-CoV-2 elicited an approximately two-fold higher stimulation innate immune response compared to SARS-CoV in epithelial line Calu-3, including induction miRNA-155. Single-cell RNA sequencing...
Abstract The coronavirus disease 2019 (COVID-19) pandemic, caused by the novel severe acute respiratory syndrome 2 (SARS-CoV-2), is an ongoing global health threat with more than two million infected people since its emergence in late 2019. Detailed knowledge of molecular biology infection indispensable for understanding viral replication, host responses, and progression. We provide gene expression profiles SARS-CoV SARS-CoV-2 infections three human cell lines (H1299, Caco-2 Calu-3 cells),...
Abstract Early stages of embryogenesis depend on subcellular localization and transport maternal mRNA. However, systematic analysis these processes is hindered by a lack spatio-temporal information in single-cell RNA sequencing. Here, we combine spatially-resolved transcriptomics labeling to perform the transcriptome during early zebrafish development. We measure spatial mRNA molecules within one-cell stage embryo, which allows us identify class mRNAs that are specifically localized at an...
Abstract In colorectal cancer, oncogenic mutations transform a hierarchically organized and homeostatic epithelium into invasive cancer tissue lacking visible organization. We sought to define cell types signals controlling their development. More than 30,000 epithelial single transcriptomes of tumors matched non-cancerous tissues twelve patients were clustered six patient-overarching groups defined by differential activities signaling pathways such as mitogen-activated protein kinase traits...
Summary Early stages of embryogenesis depend heavily on subcellular localization and transport maternally deposited mRNA. However, systematic analysis these processes is currently hindered by a lack spatio-temporal information in single-cell RNA sequencing. Here, we combined spatially-resolved transcriptomics labeling to study the dynamics transcriptome during first few hours zebrafish development. We measured spatial mRNA molecules with sub-single-cell resolution at one-cell stage, which...